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Effect of Th1 cytokines on microglia. CNS mixed glial cultures were incubated with Th1 cytokines or additional culture medium for 4 days. Cultures were fixed with 4% paraformaldehyde for 10 minutes, washed and then incubated with mouse anti-ED-1 (IgM) and mouse anti-rat MHC class II (IgG) followed by Alexa 488-conjugated goat anti-mouse IgM and Cy 3-conjugated donkey anti-mouse IgG. Cultures were examined for indirect immunofluoresence employing a Leitz Orthoplan 2 fluorescent microscope. The Th1 treated microglia (ED-1+ cells) have a different appearance when compared to those incubated with additional medium (control). Control microglia do not express MHC class II whereas Th1 treated microglia strongly express MHC class II molecules.

Differential effects of Th1, monocyte/macrophage and Th2 cytokine mixtures on early gene expression for glial and neural-related molecules in central nervous system mixed glial cell cultures: neurotrophins, growth factors and structural proteins.

Lisak RP et al. - J Neuroinflammation (2007)

Effect of Th1 cytokines on microglia. CNS mixed glial cultures were incubated with Th1 cytokines or additional culture medium for 4 days. Cultures were fixed with 4% paraformaldehyde...

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Screenshot from one of the EU-EBM e-learning modules. Screenshot showing the various audiovisual teaching modalities that are applied in the e-learning session to support self-directed learning: a) a person is talking to the learner; b) the learner can follow the presentation by reading the notes; c) the slide summarizes the core content of the presentation; it may contain hyperlinks to other topics in the same module; d) the sidebar provides orientation to the learner about the content of the lecture; e) the bottom bar allows the learner to pause, or quickly navigate forth and back.

A clinically integrated curriculum in evidence-based medicine for just-in-time learning through on-the-job training: the EU-EBM project.

Coppus SF et al. - BMC Med Educ (2007)

Screenshot from one of the EU-EBM e-learning modules. Screenshot showing the various audiovisual teaching modalities that are applied in the e-learning session to support self-directed...

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Ultrasound images of lateral ankle. (a) Un-injured control. (b) Grade I sprain 7 days post injury. The hypoechoic zone between the markers (*) indicates an area of soft tissue swelling. F = Fibula. T = Talus. ATFL = Anterior talofibular ligament. [Scale = mm]

The PRICE study (Protection Rest Ice Compression Elevation): design of a randomised controlled trial comparing standard versus cryokinetic ice applications in the management of acute ankle sprain [ISRCTN13903946].

Bleakley CM et al. - BMC Musculoskelet Disord (2007)

Ultrasound images of lateral ankle. (a) Un-injured control. (b) Grade I sprain 7 days post injury. The hypoechoic zone between the markers (*) indicates an area of soft tissue swelling....

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Cartoon representation of L. tarentolae HGPRT tertiary structure. The two monomers present in the crystal asymmetric unit are represented separately in the same orientation. Loops from the active site are labeled for monomer A and secondary structure elements are labeled for monomer B. Figures 1-6 were produced using PyMol [42].

Crystal structure of Leishmania tarentolae hypoxanthine-guanine phosphoribosyltransferase.

Monzani PS et al. - BMC Struct. Biol. (2007)

Cartoon representation of L. tarentolae HGPRT tertiary structure. The two monomers present in the crystal asymmetric unit are represented separately in the same orientation. Loops from...

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Lymphatic vessels (black arrows) were specifically stained with D2-40, an antibody against podoplanin (A, B, C, D), that did not stain blood vessels (arrow head). A (×100), B (×200), C (×100), D (×200).

How useful is the assessment of lymphatic vascular density in oral carcinoma prognosis?

Longatto Filho A et al. - World J Surg Oncol (2007)

Lymphatic vessels (black arrows) were specifically stained with D2-40, an antibody against podoplanin (A, B, C, D), that did not stain blood vessels (arrow head). A (×100), B (×200),...

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Expression of CCR2 mRNA and protein immunoreactivity in rat lumbar DRG ipsilateral to focal nerve demyelination. A) Lumbar DRG removed from vehicle-treated animals at POD7 did not exhibit CCR2 mRNA expression (n = 5). B) Many lumbar DRG neurons in vehicle-treated rats sensory neurons were positive for isolectin IB4, a neuronal phenotype that distinguishes some C-fiber nociceptors (green cells). There was no evidence of CCR2 protein expression in sham animals (n = 5). C) Lumbar DRG neurons from nerve-injured rats on POD7 exhibited CCR2 mRNA transcripts in some small and medium diameter neurons (black arrows). Open black arrowhead indicates a neuron without CCR2 mRNA transcripts (n = 4). D) Lumbar DRG neurons from a rat subjected to focal nerve demyelination exhibited few CCR2 immunopositive (white arrows) sensory neurons (n = 4). E) Many lumbar DRG neurons on POD14 exhibited CCR2 mRNA transcripts (black arrows). Open arrowhead indicates non-labeled neuron. F) CCR2 immunoreactivity was present in an increased number of neurons at POD14 (white arrows; n = 5). Scale bar is; 30 μm (A, C), 50 μm (B, D, F), and 100 μm (E).

Delayed functional expression of neuronal chemokine receptors following focal nerve demyelination in the rat: a mechanism for the development of chronic sensitization of peripheral nociceptors.

Bhangoo S et al. - Mol Pain (2007)

Expression of CCR2 mRNA and protein immunoreactivity in rat lumbar DRG ipsilateral to focal nerve demyelination. A) Lumbar DRG removed from vehicle-treated animals at POD7 did not exhibit...

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Digitising and fitting. An illustration of the nerve digitisation and fitting process. (a) Slice VM1195 from the Visible Man project with the sciatic nerve identified with a single point and arrow. (b) A fitted representation of part of sciatic nerve from the VM dataset and two of its branches- the tibial and common fibular nerves.

Anatomically based lower limb nerve model for electrical stimulation.

Kim JH et al. - Biomed Eng Online (2007)

Digitising and fitting. An illustration of the nerve digitisation and fitting process. (a) Slice VM1195 from the Visible Man project with the sciatic nerve identified with a single point...

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Dendrogram of genetic relationship among all 915 bovine animals. Neighbor-joining tree based on the pairwise genetic distances between all animals estimated by the logarithm of the proportions of shared alleles. Each tip represents a single animal and breeds are distinguished by different colors according to the legend.

Microsatellite based genetic diversity and relationships among ten Creole and commercial cattle breeds raised in Brazil.

Egito AA et al. - BMC Genet. (2007)

Dendrogram of genetic relationship among all 915 bovine animals. Neighbor-joining tree based on the pairwise genetic distances between all animals estimated by the logarithm of the proportions...

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getmorefigures.php?uid=PMC2228320&req=5

Two frames (a) and (b) from a real dynamic PET study used for the formation of a simulated dynamic PET phantom image series, (c). The TACs (d) from the study were used for the definition of the corresponding TAC functions of the phantom. The phantom consisted of a large ellipse (M) corresponding to the normal tissue mass and three smaller ellipses corresponding to the bladder (B), tumor (T), and a blood vessel (V).

Analysis and interpretation of dynamic FDG PET oncological studies using data reduction techniques.

Pavlopoulos S et al. - Biomed Eng Online (2007)

Two frames (a) and (b) from a real dynamic PET study used for the formation of a simulated dynamic PET phantom image series, (c). The TACs (d) from the study were used for the definition...

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An immunohistochemical study of altered immunomodulatory molecule expression in head and neck squamous cell carcinoma.

Vora AR, Rodgers S, Parker AJ, Start R, Rees RC, Murray AK - Br. J. Cancer (1997)

Effect of Th1 cytokines on microglia. CNS mixed glial cultures were incubated with Th1 cytokines or additional culture medium for 4 days. Cultures were fixed with 4% paraformaldehyde for 10 minutes, washed and then incubated with mouse anti-ED-1 (IgM) and mouse anti-rat MHC class II (IgG) followed by Alexa 488-conjugated goat anti-mouse IgM and Cy 3-conjugated donkey anti-mouse IgG. Cultures were examined for indirect immunofluoresence employing a Leitz Orthoplan 2 fluorescent microscope. The Th1 treated microglia (ED-1+ cells) have a different appearance when compared to those incubated with additional medium (control). Control microglia do not express MHC class II whereas Th1 treated microglia strongly express MHC class II molecules.

Differential effects of Th1, monocyte/macrophage and Th2 cytokine mixtures on early gene expression for glial and neural-related molecules in central nervous system mixed glial cell cultures: neurotrophins, growth factors and structural proteins.

Lisak RP, Benjamins JA, Bealmear B, Nedelkoska L, Yao B, Land S, Studzinski D - J Neuroinflammation (2007)

Effect of Th1 cytokines on microglia. CNS mixed glial cultures were incubated with Th1 cytokines or additional culture medium for 4 days. Cultures were fixed with 4% paraformaldehyde...
Screenshot from one of the EU-EBM e-learning modules. Screenshot showing the various audiovisual teaching modalities that are applied in the e-learning session to support self-directed learning: a) a person is talking to the learner; b) the learner can follow the presentation by reading the notes; c) the slide summarizes the core content of the presentation; it may contain hyperlinks to other topics in the same module; d) the sidebar provides orientation to the learner about the content of the lecture; e) the bottom bar allows the learner to pause, or quickly navigate forth and back.

A clinically integrated curriculum in evidence-based medicine for just-in-time learning through on-the-job training: the EU-EBM project.

Coppus SF, Emparanza JI, Hadley J, Kulier R, Weinbrenner S, Arvanitis TN, Burls A, Cabello JB, Decsi T, Horvath AR, Kaczor M, Zanrei G, Pierer K, Stawiarz K, Kunz R, Mol BW, Khan KS - BMC Med Educ (2007)

Screenshot from one of the EU-EBM e-learning modules. Screenshot showing the various audiovisual teaching modalities that are applied in the e-learning session to support self-directed...
Ultrasound images of lateral ankle. (a) Un-injured control. (b) Grade I sprain 7 days post injury. The hypoechoic zone between the markers (*) indicates an area of soft tissue swelling. F = Fibula. T = Talus. ATFL = Anterior talofibular ligament. [Scale = mm]

The PRICE study (Protection Rest Ice Compression Elevation): design of a randomised controlled trial comparing standard versus cryokinetic ice applications in the management of acute ankle sprain [ISRCTN13903946].

Bleakley CM, O'Connor S, Tully MA, Rocke LG, Macauley DC, McDonough SM - BMC Musculoskelet Disord (2007)

Ultrasound images of lateral ankle. (a) Un-injured control. (b) Grade I sprain 7 days post injury. The hypoechoic zone between the markers (*) indicates an area of soft tissue swelling....
Cartoon representation of L. tarentolae HGPRT tertiary structure. The two monomers present in the crystal asymmetric unit are represented separately in the same orientation. Loops from the active site are labeled for monomer A and secondary structure elements are labeled for monomer B. Figures 1-6 were produced using PyMol [42].

Crystal structure of Leishmania tarentolae hypoxanthine-guanine phosphoribosyltransferase.

Monzani PS, Trapani S, Thiemann OH, Oliva G - BMC Struct. Biol. (2007)

Cartoon representation of L. tarentolae HGPRT tertiary structure. The two monomers present in the crystal asymmetric unit are represented separately in the same orientation. Loops from...
Lymphatic vessels (black arrows) were specifically stained with D2-40, an antibody against podoplanin (A, B, C, D), that did not stain blood vessels (arrow head). A (×100), B (×200), C (×100), D (×200).

How useful is the assessment of lymphatic vascular density in oral carcinoma prognosis?

Longatto Filho A, Oliveira TG, Pinheiro C, de Carvalho MB, Curioni OA, Mercante AM, Schmitt FC, Gattás GJ - World J Surg Oncol (2007)

Lymphatic vessels (black arrows) were specifically stained with D2-40, an antibody against podoplanin (A, B, C, D), that did not stain blood vessels (arrow head). A (×100), B (×200),...
Expression of CCR2 mRNA and protein immunoreactivity in rat lumbar DRG ipsilateral to focal nerve demyelination. A) Lumbar DRG removed from vehicle-treated animals at POD7 did not exhibit CCR2 mRNA expression (n = 5). B) Many lumbar DRG neurons in vehicle-treated rats sensory neurons were positive for isolectin IB4, a neuronal phenotype that distinguishes some C-fiber nociceptors (green cells). There was no evidence of CCR2 protein expression in sham animals (n = 5). C) Lumbar DRG neurons from nerve-injured rats on POD7 exhibited CCR2 mRNA transcripts in some small and medium diameter neurons (black arrows). Open black arrowhead indicates a neuron without CCR2 mRNA transcripts (n = 4). D) Lumbar DRG neurons from a rat subjected to focal nerve demyelination exhibited few CCR2 immunopositive (white arrows) sensory neurons (n = 4). E) Many lumbar DRG neurons on POD14 exhibited CCR2 mRNA transcripts (black arrows). Open arrowhead indicates non-labeled neuron. F) CCR2 immunoreactivity was present in an increased number of neurons at POD14 (white arrows; n = 5). Scale bar is; 30 μm (A, C), 50 μm (B, D, F), and 100 μm (E).

Delayed functional expression of neuronal chemokine receptors following focal nerve demyelination in the rat: a mechanism for the development of chronic sensitization of peripheral nociceptors.

Bhangoo S, Ren D, Miller RJ, Henry KJ, Lineswala J, Hamdouchi C, Li B, Monahan PE, Chan DM, Ripsch MS, White FA - Mol Pain (2007)

Expression of CCR2 mRNA and protein immunoreactivity in rat lumbar DRG ipsilateral to focal nerve demyelination. A) Lumbar DRG removed from vehicle-treated animals at POD7 did not exhibit...
Digitising and fitting. An illustration of the nerve digitisation and fitting process. (a) Slice VM1195 from the Visible Man project with the sciatic nerve identified with a single point and arrow. (b) A fitted representation of part of sciatic nerve from the VM dataset and two of its branches- the tibial and common fibular nerves.

Anatomically based lower limb nerve model for electrical stimulation.

Kim JH, Davidson JB, Röhrle O, Soboleva TK, Pullan AJ - Biomed Eng Online (2007)

Digitising and fitting. An illustration of the nerve digitisation and fitting process. (a) Slice VM1195 from the Visible Man project with the sciatic nerve identified with a single point...
Dendrogram of genetic relationship among all 915 bovine animals. Neighbor-joining tree based on the pairwise genetic distances between all animals estimated by the logarithm of the proportions of shared alleles. Each tip represents a single animal and breeds are distinguished by different colors according to the legend.

Microsatellite based genetic diversity and relationships among ten Creole and commercial cattle breeds raised in Brazil.

Egito AA, Paiva SR, Albuquerque Mdo S, Mariante AS, Almeida LD, Castro SR, Grattapaglia D - BMC Genet. (2007)

Dendrogram of genetic relationship among all 915 bovine animals. Neighbor-joining tree based on the pairwise genetic distances between all animals estimated by the logarithm of the proportions...
Two frames (a) and (b) from a real dynamic PET study used for the formation of a simulated dynamic PET phantom image series, (c). The TACs (d) from the study were used for the definition of the corresponding TAC functions of the phantom. The phantom consisted of a large ellipse (M) corresponding to the normal tissue mass and three smaller ellipses corresponding to the bladder (B), tumor (T), and a blood vessel (V).

Analysis and interpretation of dynamic FDG PET oncological studies using data reduction techniques.

Pavlopoulos S, Thireou T, Kontaxakis G, Santos A - Biomed Eng Online (2007)

Two frames (a) and (b) from a real dynamic PET study used for the formation of a simulated dynamic PET phantom image series, (c). The TACs (d) from the study were used for the definition...