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Alteration in actin cytoskeleton.The actin cytoskeleton change was determined by staining with alex488 conjugated phalloidin (green). Phalloidin binds to F-actin. The nucleus and parasite was stained with DAPI (blue) and visualized by confocal microscopy. A) N-MΦ, B) I-MΦ, and C) I-MΦ-CL.

Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol.

Ghosh M et al. - PLoS Negl Trop Dis (2014)

Alteration in actin cytoskeleton.The actin cytoskeleton change was determined by staining with alex488 conjugated phalloidin (green). Phalloidin binds to F-actin. The nucleus and parasite...

Related in: Results Collection MedlinePlus

View Article: PubMed Central PubMed

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getmorefigures.php?pmc=4256160&rFormat=json&query=the&uniq=1&req=5

Giemsa-stained promastigote forms from culture.A–H, examples showing morphological variation of forms observed, all at the same magnification, bar in F represents 5 µm. Procyclic-like promastigotes can be observed in A (arrows);, leptomonad-like promastigotes in B and C (arrows); nectomonad-like promastigotes in D, E and F; and rosettes and aggregates in G and H.

First isolation of Leishmania from Northern Thailand: case report, identification as Leishmania martiniquensis and phylogenetic position within the Leishmania enriettii complex.

Pothirat T et al. - PLoS Negl Trop Dis (2014)

Giemsa-stained promastigote forms from culture.A–H, examples showing morphological variation of forms observed, all at the same magnification, bar in F represents 5 µm. Procyclic-like...

Related in: Results Collection MedlinePlus

View Article: PubMed Central PubMed

Show All Figures -  Show MeSH

getmorefigures.php?pmc=4256172&rFormat=json&query=the&uniq=1&req=5

ANA patterns in the experimental dataset: (a) coarse speckled (b) fine speckled (c) nucleolar (d) peripheral.

Staining pattern classification of antinuclear autoantibodies based on block segmentation in indirect immunofluorescence images.

Li J et al. - PLoS ONE (2014)

ANA patterns in the experimental dataset: (a) coarse speckled (b) fine speckled (c) nucleolar (d) peripheral.

Related in: Results Collection MedlinePlus

View Article: PubMed Central PubMed

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getmorefigures.php?pmc=4256175&rFormat=json&query=the&uniq=1&req=5

Fluorescence recovery after photobleaching analysis after DNA damage induction in UV-irradiated cells.LiMRE11-GFP is recruited to DNA damage sites in human MRE11-deficient cells (ATLD), as the human MRE11-GFP.

Formation of linear amplicons with inverted duplications in Leishmania requires the MRE11 nuclease.

Laffitte MC et al. - PLoS Genet. (2014)

Fluorescence recovery after photobleaching analysis after DNA damage induction in UV-irradiated cells.LiMRE11-GFP is recruited to DNA damage sites in human MRE11-deficient cells (ATLD),...

Related in: Results Collection

View Article: PubMed Central PubMed

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getmorefigures.php?pmc=4256157&rFormat=json&query=the&uniq=1&req=5

Wood decay characteristics.Comparative weight loss of parental strain 11061 and single basidiospore derivatives on colonized loblolly pine wood (Pinus taeda) wood wafers were determined after 4, 8 and 12 weeks incubation (bottom left panel) as described in Methods. Single basidiospore strain 5–6 also aggressively decayed birch and spruce (Text S1) and was selected for sequencing. Upper panels show scanning electron microscopy [68] of radial (left) and transverse (right) sections of pine wood tracheids that were substantially eroded or completely degraded by P. gigantea strain 5–6 by week twelve. Transverse section of sound wood (bottom photo) provides comparison. (Bar  = 40 µm).

Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood.

Hori C et al. - PLoS Genet. (2014)

Wood decay characteristics.Comparative weight loss of parental strain 11061 and single basidiospore derivatives on colonized loblolly pine wood (Pinus taeda) wood wafers were determined...

Related in: Results Collection MedlinePlus

View Article: PubMed Central PubMed

Show All Figures -  Show MeSH

getmorefigures.php?pmc=4256170&rFormat=json&query=the&uniq=1&req=5

Co-incubation with macrophages led to regained filamentation in the cph1Δ/efg1Δ strain.Morphology of wild type (WT), cph1Δ/efg1Δ and Evo strain after 18 h of incubation in DMEM+10% FBS at 37°C and 5% CO2 demonstrate the re-appearance of filamentation in the Evo strain (scale bar: 100 µm, upper panel). All strains were grown for 4 h for filipin (Fil) staining, and for 2 h or 16 h for calcofluor white (CFW) staining on cover slips, and analyzed by fluorescence microscopy (scale bar: 10 µm, lower panels). Arrow heads highlight septa (true hyphae), while asterisks indicate constrictions (pseudohyphae).

Microevolution of Candida albicans in macrophages restores filamentation in a nonfilamentous mutant.

Wartenberg A et al. - PLoS Genet. (2014)

Co-incubation with macrophages led to regained filamentation in the cph1Δ/efg1Δ strain.Morphology of wild type (WT), cph1Δ/efg1Δ and Evo strain after 18 h of incubation in DMEM+10%...

Related in: Results Collection MedlinePlus

View Article: PubMed Central PubMed

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getmorefigures.php?pmc=4256171&rFormat=json&query=the&uniq=1&req=5

Validation of assay.A–C. Wing discs expressing (A) no double-stranded RNA (control), (B) double-stranded RNA targeting smo, and (C) double-stranded RNA targeting ci in clones of cells marked by the expression of DsRed (red). Cells of the posterior compartment are labeled by expression of Venus under control of the engrailed gene (en-Venus, green). In (B) and (C), anterior clones along the AP boundary mis-segregate into the posterior territory of the wing disc (asterisks). Scale bar is 50 µm.

An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.

Umetsu D et al. - PLoS ONE (2014)

Validation of assay.A–C. Wing discs expressing (A) no double-stranded RNA (control), (B) double-stranded RNA targeting smo, and (C) double-stranded RNA targeting ci in clones of cells...

Related in: Results Collection

View Article: PubMed Central PubMed

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getmorefigures.php?pmc=4256218&rFormat=json&query=the&uniq=1&req=5

Histopathology of the organs collected from the immunized group animals on 3rd and 20th day post infection with Y. pestis and the naive control animals that were neither immunized nor challenged with Y. pestis.Tissue sections were stained with hematoxylin and eosin for pathological examination. Tissue section collected from naive control and immunized animals on 3rd day post infection i.e., Naive control (A); PBS control (B); HSP70(II) (C); F1 (D); F1+HSP70(II) (E); LcrV (F); LcrV+HSP70(II) (G); F1+LcrV (H); F1+LcrV+HSP70(II) (I). Tissue sections were collected from the survived animal groups on 20th day post infection i.e., LcrV (J); LcrV+HSP70(II) (K); F1+LcrV (L); F1+LcrV+HSP70(II) (M). Photomicrograph represents the histopathology of Lung[a]: the arrows in the panel B indicate the infiltration of neutrophils. Photomicrograph of spleen [b]: in the panel B, reduced density of white pulp follicle and congestion in the red pulp, lymphoid follicle depletion shown by arrow and the presence of megakaryocytes shown by bold arrow. Photomicrograph of kidney [c]: the granular degeneration of parenchyma was observed in the panel B (bold arrows) and swelling in renal tubules (arrows). Photomicrograph of liver [d]: in the panel B, the hepatocytes degeneration was observed as indicated by arrow.

HSP70 domain II of Mycobacterium tuberculosis modulates immune response and protective potential of F1 and LcrV antigens of Yersinia pestis in a mouse model.

Batra L et al. - PLoS Negl Trop Dis (2014)

Histopathology of the organs collected from the immunized group animals on 3rd and 20th day post infection with Y. pestis and the naive control animals that were neither immunized nor...

Related in: Results Collection MedlinePlus

View Article: PubMed Central PubMed

Show All Figures -  Show MeSH

getmorefigures.php?pmc=4256173&rFormat=json&query=the&uniq=1&req=5

Flexibility test system.The specimen was fixed on the working table. Laser balls were used to simulate planes of representative vertebral bodies. An unstrained spinal 3-D motion was obtained by cables connected to loading discs. A suspended counterweight transferred equal but opposing forces on to the top of the specimen. The movement of the laser balls was tracked by six cameras hanging at different sites from the ceiling.

Biomechanical analysis of a newly developed shape memory alloy hook in a transforaminal lumbar interbody fusion (TLIF) in vitro model.

Wang X et al. - PLoS ONE (2014)

Flexibility test system.The specimen was fixed on the working table. Laser balls were used to simulate planes of representative vertebral bodies. An unstrained spinal 3-D motion was obtained...

Related in: Results Collection MedlinePlus

View Article: PubMed Central PubMed

Show All Figures -  Show MeSH

getmorefigures.php?pmc=4256230&rFormat=json&query=the&uniq=1&req=5

EdU proliferation assay analysis of the effect of IGF1R-targeted miR-143/145 on the growth of colorectal cancer cells.The red fluorescent cells are in the S phase of mitosis, and the blue fluorescent cells represent all of the cells. (A and B) The EdU proliferation assay was performed 48 h after the transfection of Caco2 cells with a scrambled control, pre-miR-143, pre-miR-145 or both pre-miR-143 and pre-miR-145. A: representative image; B: ratio of EdU-positive Caco2 cells. (C and D) The EdU proliferation assay was performed 48 h after the transfection of Caco2 cells with a scrambled control siRNA, IGF1R siRNA, control vector or the IGF1R overexpression vector. C: representative image; D: ratio of EdU-positive Caco2 cells. (E and F) The EdU proliferation assay was performed 48 h after the transfection of Caco2 cells with a scrambled control plus control vector, a scrambled control plus IFG1R overexpression vector, pre-miR-143/145 plus control vector, or pre-miR-143/145 plus IFG1R overexpression vector. E: representative image; F: ratio of EdU-positive Caco2 cells. *P<0.05; **P<0.01.

MiR-143 and MiR-145 regulate IGF1R to suppress cell proliferation in colorectal cancer.

Su J et al. - PLoS ONE (2014)

EdU proliferation assay analysis of the effect of IGF1R-targeted miR-143/145 on the growth of colorectal cancer cells.The red fluorescent cells are in the S phase of mitosis, and the...

Related in: Results Collection MedlinePlus

View Article: PubMed Central PubMed

Show All Figures -  Show MeSH

getmorefigures.php?pmc=4256231&rFormat=json&query=the&uniq=1&req=5

Alteration in actin cytoskeleton.The actin cytoskeleton change was determined by staining with alex488 conjugated phalloidin (green). Phalloidin binds to F-actin. The nucleus and parasite was stained with DAPI (blue) and visualized by confocal microscopy. A) N-MΦ, B) I-MΦ, and C) I-MΦ-CL.

Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol.

Ghosh M, Roy K, Das Mukherjee D, Chakrabarti G, Roy Choudhury K, Roy S - PLoS Negl Trop Dis (2014)

Alteration in actin cytoskeleton.The actin cytoskeleton change was determined by staining with alex488 conjugated phalloidin (green). Phalloidin binds to F-actin. The nucleus and parasite...
Giemsa-stained promastigote forms from culture.A–H, examples showing morphological variation of forms observed, all at the same magnification, bar in F represents 5 µm. Procyclic-like promastigotes can be observed in A (arrows);, leptomonad-like promastigotes in B and C (arrows); nectomonad-like promastigotes in D, E and F; and rosettes and aggregates in G and H.

First isolation of Leishmania from Northern Thailand: case report, identification as Leishmania martiniquensis and phylogenetic position within the Leishmania enriettii complex.

Pothirat T, Tantiworawit A, Chaiwarith R, Jariyapan N, Wannasan A, Siriyasatien P, Supparatpinyo K, Bates MD, Kwakye-Nuako G, Bates PA - PLoS Negl Trop Dis (2014)

Giemsa-stained promastigote forms from culture.A–H, examples showing morphological variation of forms observed, all at the same magnification, bar in F represents 5 µm. Procyclic-like...
ANA patterns in the experimental dataset: (a) coarse speckled (b) fine speckled (c) nucleolar (d) peripheral.

Staining pattern classification of antinuclear autoantibodies based on block segmentation in indirect immunofluorescence images.

Li J, Tseng KK, Hsieh ZY, Yang CW, Huang HN - PLoS ONE (2014)

ANA patterns in the experimental dataset: (a) coarse speckled (b) fine speckled (c) nucleolar (d) peripheral.
Fluorescence recovery after photobleaching analysis after DNA damage induction in UV-irradiated cells.LiMRE11-GFP is recruited to DNA damage sites in human MRE11-deficient cells (ATLD), as the human MRE11-GFP.

Formation of linear amplicons with inverted duplications in Leishmania requires the MRE11 nuclease.

Laffitte MC, Genois MM, Mukherjee A, Légaré D, Masson JY, Ouellette M - PLoS Genet. (2014)

Fluorescence recovery after photobleaching analysis after DNA damage induction in UV-irradiated cells.LiMRE11-GFP is recruited to DNA damage sites in human MRE11-deficient cells (ATLD),...
Wood decay characteristics.Comparative weight loss of parental strain 11061 and single basidiospore derivatives on colonized loblolly pine wood (Pinus taeda) wood wafers were determined after 4, 8 and 12 weeks incubation (bottom left panel) as described in Methods. Single basidiospore strain 5–6 also aggressively decayed birch and spruce (Text S1) and was selected for sequencing. Upper panels show scanning electron microscopy [68] of radial (left) and transverse (right) sections of pine wood tracheids that were substantially eroded or completely degraded by P. gigantea strain 5–6 by week twelve. Transverse section of sound wood (bottom photo) provides comparison. (Bar  = 40 µm).

Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood.

Hori C, Ishida T, Igarashi K, Samejima M, Suzuki H, Master E, Ferreira P, Ruiz-Dueñas FJ, Held B, Canessa P, Larrondo LF, Schmoll M, Druzhinina IS, Kubicek CP, Gaskell JA, Kersten P, St John F, Glasner J, Sabat G, Splinter BonDurant S, Syed K, Yadav J, Mgbeahuruike AC, Kovalchuk A, Asiegbu FO, Lackner G, Hoffmeister D, Rencoret J, Gutiérrez A, Sun H, Lindquist E, Barry K, Riley R, Grigoriev IV, Henrissat B, Kües U, Berka RM, Martínez AT, Covert SF, Blanchette RA, Cullen D - PLoS Genet. (2014)

Wood decay characteristics.Comparative weight loss of parental strain 11061 and single basidiospore derivatives on colonized loblolly pine wood (Pinus taeda) wood wafers were determined...
Co-incubation with macrophages led to regained filamentation in the cph1Δ/efg1Δ strain.Morphology of wild type (WT), cph1Δ/efg1Δ and Evo strain after 18 h of incubation in DMEM+10% FBS at 37°C and 5% CO2 demonstrate the re-appearance of filamentation in the Evo strain (scale bar: 100 µm, upper panel). All strains were grown for 4 h for filipin (Fil) staining, and for 2 h or 16 h for calcofluor white (CFW) staining on cover slips, and analyzed by fluorescence microscopy (scale bar: 10 µm, lower panels). Arrow heads highlight septa (true hyphae), while asterisks indicate constrictions (pseudohyphae).

Microevolution of Candida albicans in macrophages restores filamentation in a nonfilamentous mutant.

Wartenberg A, Linde J, Martin R, Schreiner M, Horn F, Jacobsen ID, Je S, Wolf T, Kuchler K, Guthke R, Kurzai O, Forche A, d'Enfert C, Brunke S, Hube B - PLoS Genet. (2014)

Co-incubation with macrophages led to regained filamentation in the cph1Δ/efg1Δ strain.Morphology of wild type (WT), cph1Δ/efg1Δ and Evo strain after 18 h of incubation in DMEM+10%...
Validation of assay.A–C. Wing discs expressing (A) no double-stranded RNA (control), (B) double-stranded RNA targeting smo, and (C) double-stranded RNA targeting ci in clones of cells marked by the expression of DsRed (red). Cells of the posterior compartment are labeled by expression of Venus under control of the engrailed gene (en-Venus, green). In (B) and (C), anterior clones along the AP boundary mis-segregate into the posterior territory of the wing disc (asterisks). Scale bar is 50 µm.

An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.

Umetsu D, Dunst S, Dahmann C - PLoS ONE (2014)

Validation of assay.A–C. Wing discs expressing (A) no double-stranded RNA (control), (B) double-stranded RNA targeting smo, and (C) double-stranded RNA targeting ci in clones of cells...
Histopathology of the organs collected from the immunized group animals on 3rd and 20th day post infection with Y. pestis and the naive control animals that were neither immunized nor challenged with Y. pestis.Tissue sections were stained with hematoxylin and eosin for pathological examination. Tissue section collected from naive control and immunized animals on 3rd day post infection i.e., Naive control (A); PBS control (B); HSP70(II) (C); F1 (D); F1+HSP70(II) (E); LcrV (F); LcrV+HSP70(II) (G); F1+LcrV (H); F1+LcrV+HSP70(II) (I). Tissue sections were collected from the survived animal groups on 20th day post infection i.e., LcrV (J); LcrV+HSP70(II) (K); F1+LcrV (L); F1+LcrV+HSP70(II) (M). Photomicrograph represents the histopathology of Lung[a]: the arrows in the panel B indicate the infiltration of neutrophils. Photomicrograph of spleen [b]: in the panel B, reduced density of white pulp follicle and congestion in the red pulp, lymphoid follicle depletion shown by arrow and the presence of megakaryocytes shown by bold arrow. Photomicrograph of kidney [c]: the granular degeneration of parenchyma was observed in the panel B (bold arrows) and swelling in renal tubules (arrows). Photomicrograph of liver [d]: in the panel B, the hepatocytes degeneration was observed as indicated by arrow.

HSP70 domain II of Mycobacterium tuberculosis modulates immune response and protective potential of F1 and LcrV antigens of Yersinia pestis in a mouse model.

Batra L, Verma SK, Nagar DP, Saxena N, Pathak P, Pant SC, Tuteja U - PLoS Negl Trop Dis (2014)

Histopathology of the organs collected from the immunized group animals on 3rd and 20th day post infection with Y. pestis and the naive control animals that were neither immunized nor...
Flexibility test system.The specimen was fixed on the working table. Laser balls were used to simulate planes of representative vertebral bodies. An unstrained spinal 3-D motion was obtained by cables connected to loading discs. A suspended counterweight transferred equal but opposing forces on to the top of the specimen. The movement of the laser balls was tracked by six cameras hanging at different sites from the ceiling.

Biomechanical analysis of a newly developed shape memory alloy hook in a transforaminal lumbar interbody fusion (TLIF) in vitro model.

Wang X, Xu J, Zhu Y, Li J, Zhou S, Tian S, Xiang Y, Liu X, Zheng Y, Pan T - PLoS ONE (2014)

Flexibility test system.The specimen was fixed on the working table. Laser balls were used to simulate planes of representative vertebral bodies. An unstrained spinal 3-D motion was obtained...
EdU proliferation assay analysis of the effect of IGF1R-targeted miR-143/145 on the growth of colorectal cancer cells.The red fluorescent cells are in the S phase of mitosis, and the blue fluorescent cells represent all of the cells. (A and B) The EdU proliferation assay was performed 48 h after the transfection of Caco2 cells with a scrambled control, pre-miR-143, pre-miR-145 or both pre-miR-143 and pre-miR-145. A: representative image; B: ratio of EdU-positive Caco2 cells. (C and D) The EdU proliferation assay was performed 48 h after the transfection of Caco2 cells with a scrambled control siRNA, IGF1R siRNA, control vector or the IGF1R overexpression vector. C: representative image; D: ratio of EdU-positive Caco2 cells. (E and F) The EdU proliferation assay was performed 48 h after the transfection of Caco2 cells with a scrambled control plus control vector, a scrambled control plus IFG1R overexpression vector, pre-miR-143/145 plus control vector, or pre-miR-143/145 plus IFG1R overexpression vector. E: representative image; F: ratio of EdU-positive Caco2 cells. *P<0.05; **P<0.01.

MiR-143 and MiR-145 regulate IGF1R to suppress cell proliferation in colorectal cancer.

Su J, Liang H, Yao W, Wang N, Zhang S, Yan X, Feng H, Pang W, Wang Y, Wang X, Fu Z, Liu Y, Zhao C, Zhang J, Zhang CY, Zen K, Chen X, Wang Y - PLoS ONE (2014)

EdU proliferation assay analysis of the effect of IGF1R-targeted miR-143/145 on the growth of colorectal cancer cells.The red fluorescent cells are in the S phase of mitosis, and the...

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