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Identification and preliminary characterization of mouse Adam33.

Gunn TM, Azarani A, Kim PH, Hyman RW, Davis RW, Barsh GS - BMC Genet. (2002)

Bottom Line: The metalloprotease-disintegrin family, or ADAM, proteins, are implicated in cell-cell interactions, cell fusion, and cell signaling, and are widely distributed among metazoan phyla.Orthologous relationships have been defined for a few ADAM proteins including ADAM10 (Kuzbanian), and ADAM17 (TACE), but evolutionary relationships are not clear for the majority of family members.Adam33 is expressed in the mouse adult brain and could play a role in complex processes that require cell-cell communication.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pediatrics, and the Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA, USA.

ABSTRACT

Background: The metalloprotease-disintegrin family, or ADAM, proteins, are implicated in cell-cell interactions, cell fusion, and cell signaling, and are widely distributed among metazoan phyla. Orthologous relationships have been defined for a few ADAM proteins including ADAM10 (Kuzbanian), and ADAM17 (TACE), but evolutionary relationships are not clear for the majority of family members. Human ADAM33 refers to a testis cDNA clone that does not contain a complete open reading frame, but portions of the predicted protein are similar to Xenopus laevis ADAM13.

Results: In a 48 kb region of mouse DNA adjacent to the Attractin gene on mouse chromosome 2, we identified sequences very similar to human ADAM33. A full-length mouse cDNA was identified by a combination of gene prediction programs and RT-PCR, and the probable full-length human cDNA was identified by comparison to human genomic sequence in the homologous region on chromosome 20p13. Mouse ADAM33 is 44% identical to Xenopus laevis ADAM13, however a phylogenetic alignment and consideration of functional domains suggests that the two genes are not orthologous. Mouse Adam33 is widely expressed, most highly in the adult brain, heart, kidney, lung and testis.

Conclusions: While mouse ADAM33 is similar to Xenopus ADAM13 in sequence, further examination of its embryonic expression pattern, catalytic activity and protein interactions will be required to assess the functional relationship between these two proteins. Adam33 is expressed in the mouse adult brain and could play a role in complex processes that require cell-cell communication.

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Expression pattern of Adam33 in adult mouse brain. (A, D) Photomicrographs of coronal sections of adult mouse brain, from the Mouse Brain Library [25],[26]. Boxes indicate approximate regions shown in panels B-C and E-F. (B-C and E-F) In situ hybridization of digoxigenin labeled antisense (B, E) and sense (C, F) RNA probes derived from IMAGE clone 636599. Adam33 expression was observed only in the dentate gyrus (DG) and pyramidal cell layer (Py) of the hippocampus (panel B) and the granule layer (Gr) of the cerebellum (panel E), indicated by arrows.
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Figure 4: Expression pattern of Adam33 in adult mouse brain. (A, D) Photomicrographs of coronal sections of adult mouse brain, from the Mouse Brain Library [25],[26]. Boxes indicate approximate regions shown in panels B-C and E-F. (B-C and E-F) In situ hybridization of digoxigenin labeled antisense (B, E) and sense (C, F) RNA probes derived from IMAGE clone 636599. Adam33 expression was observed only in the dentate gyrus (DG) and pyramidal cell layer (Py) of the hippocampus (panel B) and the granule layer (Gr) of the cerebellum (panel E), indicated by arrows.

Mentions: The expression pattern of Adam33 in the adult brain was also examined by in situ hybridization of coronal brain sections (Figure 4). A striking pattern of hybridization was observed in the granule later of the dentate gyrus, the pyramidal layer of the hippocampus (Figure 4B), and the granule layer of the cerebellum (Figure 4E). The expression of Adam33 in two of the primary locations of granule neurons in the brain is intriguing and it will be interesting to follow expression of this gene during neuronal migration; while Xenopus ADAM13 has been implicated in neural crest cell migration during embryonic development [18], perhaps ADAM33 plays a role in the migration of granule neurons in the mouse brain. In Drosophila, the ADAM protein kuzbanian (orthologous to mammalian ADAM 10) is required for proteolytic cleavage of notch and delta, associates with a number of molecules that have been implicated in axon guidance and migration, and has been shown to play a role in axon guidance [6,19-21].


Identification and preliminary characterization of mouse Adam33.

Gunn TM, Azarani A, Kim PH, Hyman RW, Davis RW, Barsh GS - BMC Genet. (2002)

Expression pattern of Adam33 in adult mouse brain. (A, D) Photomicrographs of coronal sections of adult mouse brain, from the Mouse Brain Library [25],[26]. Boxes indicate approximate regions shown in panels B-C and E-F. (B-C and E-F) In situ hybridization of digoxigenin labeled antisense (B, E) and sense (C, F) RNA probes derived from IMAGE clone 636599. Adam33 expression was observed only in the dentate gyrus (DG) and pyramidal cell layer (Py) of the hippocampus (panel B) and the granule layer (Gr) of the cerebellum (panel E), indicated by arrows.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC88886&req=5

Figure 4: Expression pattern of Adam33 in adult mouse brain. (A, D) Photomicrographs of coronal sections of adult mouse brain, from the Mouse Brain Library [25],[26]. Boxes indicate approximate regions shown in panels B-C and E-F. (B-C and E-F) In situ hybridization of digoxigenin labeled antisense (B, E) and sense (C, F) RNA probes derived from IMAGE clone 636599. Adam33 expression was observed only in the dentate gyrus (DG) and pyramidal cell layer (Py) of the hippocampus (panel B) and the granule layer (Gr) of the cerebellum (panel E), indicated by arrows.
Mentions: The expression pattern of Adam33 in the adult brain was also examined by in situ hybridization of coronal brain sections (Figure 4). A striking pattern of hybridization was observed in the granule later of the dentate gyrus, the pyramidal layer of the hippocampus (Figure 4B), and the granule layer of the cerebellum (Figure 4E). The expression of Adam33 in two of the primary locations of granule neurons in the brain is intriguing and it will be interesting to follow expression of this gene during neuronal migration; while Xenopus ADAM13 has been implicated in neural crest cell migration during embryonic development [18], perhaps ADAM33 plays a role in the migration of granule neurons in the mouse brain. In Drosophila, the ADAM protein kuzbanian (orthologous to mammalian ADAM 10) is required for proteolytic cleavage of notch and delta, associates with a number of molecules that have been implicated in axon guidance and migration, and has been shown to play a role in axon guidance [6,19-21].

Bottom Line: The metalloprotease-disintegrin family, or ADAM, proteins, are implicated in cell-cell interactions, cell fusion, and cell signaling, and are widely distributed among metazoan phyla.Orthologous relationships have been defined for a few ADAM proteins including ADAM10 (Kuzbanian), and ADAM17 (TACE), but evolutionary relationships are not clear for the majority of family members.Adam33 is expressed in the mouse adult brain and could play a role in complex processes that require cell-cell communication.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pediatrics, and the Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA, USA.

ABSTRACT

Background: The metalloprotease-disintegrin family, or ADAM, proteins, are implicated in cell-cell interactions, cell fusion, and cell signaling, and are widely distributed among metazoan phyla. Orthologous relationships have been defined for a few ADAM proteins including ADAM10 (Kuzbanian), and ADAM17 (TACE), but evolutionary relationships are not clear for the majority of family members. Human ADAM33 refers to a testis cDNA clone that does not contain a complete open reading frame, but portions of the predicted protein are similar to Xenopus laevis ADAM13.

Results: In a 48 kb region of mouse DNA adjacent to the Attractin gene on mouse chromosome 2, we identified sequences very similar to human ADAM33. A full-length mouse cDNA was identified by a combination of gene prediction programs and RT-PCR, and the probable full-length human cDNA was identified by comparison to human genomic sequence in the homologous region on chromosome 20p13. Mouse ADAM33 is 44% identical to Xenopus laevis ADAM13, however a phylogenetic alignment and consideration of functional domains suggests that the two genes are not orthologous. Mouse Adam33 is widely expressed, most highly in the adult brain, heart, kidney, lung and testis.

Conclusions: While mouse ADAM33 is similar to Xenopus ADAM13 in sequence, further examination of its embryonic expression pattern, catalytic activity and protein interactions will be required to assess the functional relationship between these two proteins. Adam33 is expressed in the mouse adult brain and could play a role in complex processes that require cell-cell communication.

Show MeSH
Related in: MedlinePlus