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Genomic Regions That Underlie Soybean Seed Isoflavone Content.

Meksem K, Njiti VN, Banz WJ, Iqbal MJ, Kassem MM, Hyten DL, Yuang J, Winters TA, Lightfoot DA - J. Biomed. Biotechnol. (2001)

Bottom Line: Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect.Four genomic regions were found to be significantly associated with the isoflavone content of soybean seeds across both locations and years.In addition, tightly linked markers can be used in map based cloning of genes associated with isoflavone content.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Soy products contain isoflavones (genistein, daidzein, and glycitein) that display biological effects when ingested by humans and animals, these effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect. Our objective was to identify loci that underlie isoflavone content in soybean seeds. The study involved 100 recombinant inbred lines (RIL) from the cross of 'Essex' by 'Forrest,' two cultivars that contrast for isoflavone content. Isoflavone content of seeds from each RIL was determined by high performance liquid chromatography (HPLC). The distribution of isoflavone content was continuous and unimodal. The heritability estimates on a line mean basis were 79% for daidzein, 22% for genistein, and 88% for glycitein. Isoflavone content of soybean seeds was compared against 150 polymorphic DNA markers in a one-way analysis of variance. Four genomic regions were found to be significantly associated with the isoflavone content of soybean seeds across both locations and years. Molecular linkage group B1 contained a major QTL underlying glycitein content (P = 0.0001, R(2) = 50.2%), linkage group N contained a QTL for glycitein (P = 0.0033, R(2) = 11.1%) and a QTL for daidzein (P = 0.0023, R(2) = 10.3%) and linkage group A1 contained a QTL for daidzein (P = 0.0081, R(2) = 9.6%). Selection for these chromosomal regions in a marker assisted selection program will allow for the manipulation of amounts and profiles of isoflavones (genistein, daidzein, and glycitein) content of soybean seeds. In addition, tightly linked markers can be used in map based cloning of genes associated with isoflavone content.

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Related in: MedlinePlus

Location of microsatellite markers and QTL that conditionphytoestrogen content. END indicates the likely position of thetelomere on that linkage group, the disjunct bar represents therest of the linkage group. Marker names and distances and peakLOD score for the interval are given. LOD scores are from singlelocus analyses of additive gene effects using Mapmaker/QTL 1.1.Genetic distances were from the recombinant inbred line functionof Mapmaker/EXP 3.0.
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Figure 3: Location of microsatellite markers and QTL that conditionphytoestrogen content. END indicates the likely position of thetelomere on that linkage group, the disjunct bar represents therest of the linkage group. Marker names and distances and peakLOD score for the interval are given. LOD scores are from singlelocus analyses of additive gene effects using Mapmaker/QTL 1.1.Genetic distances were from the recombinant inbred line functionof Mapmaker/EXP 3.0.

Mentions: Using the whole population of 100 RILs, with data from pooledplot replicates, four chromosomal regions on three differentmolecular linkage groups were found to contain QTL for seedisoflavone content (Table 1 and Figure 3). Aregion on linkage group B1 identified previously in thesubpopulations of 40 and 60 RILs by the microsatellitemarker Satt251 was again significantly (P = 0.0001, R2 = 49%)associated with glycitein content. The linked markers Satt197 andSatt415 were also significantly associated with glyciteincontent. The interval containing the QTL spanned about 10 cMand had a peaked LOD score of 10.6 and explained about 51% oftotal variation in glycitein content. The region derived thebeneficial allele from Essex.


Genomic Regions That Underlie Soybean Seed Isoflavone Content.

Meksem K, Njiti VN, Banz WJ, Iqbal MJ, Kassem MM, Hyten DL, Yuang J, Winters TA, Lightfoot DA - J. Biomed. Biotechnol. (2001)

Location of microsatellite markers and QTL that conditionphytoestrogen content. END indicates the likely position of thetelomere on that linkage group, the disjunct bar represents therest of the linkage group. Marker names and distances and peakLOD score for the interval are given. LOD scores are from singlelocus analyses of additive gene effects using Mapmaker/QTL 1.1.Genetic distances were from the recombinant inbred line functionof Mapmaker/EXP 3.0.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC79679&req=5

Figure 3: Location of microsatellite markers and QTL that conditionphytoestrogen content. END indicates the likely position of thetelomere on that linkage group, the disjunct bar represents therest of the linkage group. Marker names and distances and peakLOD score for the interval are given. LOD scores are from singlelocus analyses of additive gene effects using Mapmaker/QTL 1.1.Genetic distances were from the recombinant inbred line functionof Mapmaker/EXP 3.0.
Mentions: Using the whole population of 100 RILs, with data from pooledplot replicates, four chromosomal regions on three differentmolecular linkage groups were found to contain QTL for seedisoflavone content (Table 1 and Figure 3). Aregion on linkage group B1 identified previously in thesubpopulations of 40 and 60 RILs by the microsatellitemarker Satt251 was again significantly (P = 0.0001, R2 = 49%)associated with glycitein content. The linked markers Satt197 andSatt415 were also significantly associated with glyciteincontent. The interval containing the QTL spanned about 10 cMand had a peaked LOD score of 10.6 and explained about 51% oftotal variation in glycitein content. The region derived thebeneficial allele from Essex.

Bottom Line: Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect.Four genomic regions were found to be significantly associated with the isoflavone content of soybean seeds across both locations and years.In addition, tightly linked markers can be used in map based cloning of genes associated with isoflavone content.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Soy products contain isoflavones (genistein, daidzein, and glycitein) that display biological effects when ingested by humans and animals, these effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect. Our objective was to identify loci that underlie isoflavone content in soybean seeds. The study involved 100 recombinant inbred lines (RIL) from the cross of 'Essex' by 'Forrest,' two cultivars that contrast for isoflavone content. Isoflavone content of seeds from each RIL was determined by high performance liquid chromatography (HPLC). The distribution of isoflavone content was continuous and unimodal. The heritability estimates on a line mean basis were 79% for daidzein, 22% for genistein, and 88% for glycitein. Isoflavone content of soybean seeds was compared against 150 polymorphic DNA markers in a one-way analysis of variance. Four genomic regions were found to be significantly associated with the isoflavone content of soybean seeds across both locations and years. Molecular linkage group B1 contained a major QTL underlying glycitein content (P = 0.0001, R(2) = 50.2%), linkage group N contained a QTL for glycitein (P = 0.0033, R(2) = 11.1%) and a QTL for daidzein (P = 0.0023, R(2) = 10.3%) and linkage group A1 contained a QTL for daidzein (P = 0.0081, R(2) = 9.6%). Selection for these chromosomal regions in a marker assisted selection program will allow for the manipulation of amounts and profiles of isoflavones (genistein, daidzein, and glycitein) content of soybean seeds. In addition, tightly linked markers can be used in map based cloning of genes associated with isoflavone content.

No MeSH data available.


Related in: MedlinePlus