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Genomic Regions That Underlie Soybean Seed Isoflavone Content.

Meksem K, Njiti VN, Banz WJ, Iqbal MJ, Kassem MM, Hyten DL, Yuang J, Winters TA, Lightfoot DA - J. Biomed. Biotechnol. (2001)

Bottom Line: Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect.Four genomic regions were found to be significantly associated with the isoflavone content of soybean seeds across both locations and years.In addition, tightly linked markers can be used in map based cloning of genes associated with isoflavone content.

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ABSTRACT
Soy products contain isoflavones (genistein, daidzein, and glycitein) that display biological effects when ingested by humans and animals, these effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect. Our objective was to identify loci that underlie isoflavone content in soybean seeds. The study involved 100 recombinant inbred lines (RIL) from the cross of 'Essex' by 'Forrest,' two cultivars that contrast for isoflavone content. Isoflavone content of seeds from each RIL was determined by high performance liquid chromatography (HPLC). The distribution of isoflavone content was continuous and unimodal. The heritability estimates on a line mean basis were 79% for daidzein, 22% for genistein, and 88% for glycitein. Isoflavone content of soybean seeds was compared against 150 polymorphic DNA markers in a one-way analysis of variance. Four genomic regions were found to be significantly associated with the isoflavone content of soybean seeds across both locations and years. Molecular linkage group B1 contained a major QTL underlying glycitein content (P = 0.0001, R(2) = 50.2%), linkage group N contained a QTL for glycitein (P = 0.0033, R(2) = 11.1%) and a QTL for daidzein (P = 0.0023, R(2) = 10.3%) and linkage group A1 contained a QTL for daidzein (P = 0.0081, R(2) = 9.6%). Selection for these chromosomal regions in a marker assisted selection program will allow for the manipulation of amounts and profiles of isoflavones (genistein, daidzein, and glycitein) content of soybean seeds. In addition, tightly linked markers can be used in map based cloning of genes associated with isoflavone content.

No MeSH data available.


Autoradiograph showing PCR amplification of genomic DNA from the low glyciteinForrest (F), the high glycitein parent (E) and the F5:13selected RIL segregating for glycitein soybean seed content withthe BARC-Sat251 primers. The labeled PCR products wereelectrophoresed on an 5% polyacrylamide denaturing gel (H: Highcontent, L: Low content of glycitein seed content.
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Figure 2: Autoradiograph showing PCR amplification of genomic DNA from the low glyciteinForrest (F), the high glycitein parent (E) and the F5:13selected RIL segregating for glycitein soybean seed content withthe BARC-Sat251 primers. The labeled PCR products wereelectrophoresed on an 5% polyacrylamide denaturing gel (H: Highcontent, L: Low content of glycitein seed content.

Mentions: Using subpopulation of 40 RILs with data from each plotreplicate, three chromosomal region on two different molecularlinkage groups were found to contain quantitative trait loci(QTL) for seed isoflavone content (Table 1). A regionon linkage group B1 identified by the microsatellite markerSatt251 was significantly (P = 0.0001, R2 = 49%) associatedwith glycitein content (Figure 2). The linked markersSatt197 and Satt415 were also significantly associated withglycitein content. The interval containing the QTL spanning about10 cM had LOD score of 10.6 and explained about 50% of totalvariation in glycitein content. The region derived the beneficialallele from Essex.


Genomic Regions That Underlie Soybean Seed Isoflavone Content.

Meksem K, Njiti VN, Banz WJ, Iqbal MJ, Kassem MM, Hyten DL, Yuang J, Winters TA, Lightfoot DA - J. Biomed. Biotechnol. (2001)

Autoradiograph showing PCR amplification of genomic DNA from the low glyciteinForrest (F), the high glycitein parent (E) and the F5:13selected RIL segregating for glycitein soybean seed content withthe BARC-Sat251 primers. The labeled PCR products wereelectrophoresed on an 5% polyacrylamide denaturing gel (H: Highcontent, L: Low content of glycitein seed content.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC79679&req=5

Figure 2: Autoradiograph showing PCR amplification of genomic DNA from the low glyciteinForrest (F), the high glycitein parent (E) and the F5:13selected RIL segregating for glycitein soybean seed content withthe BARC-Sat251 primers. The labeled PCR products wereelectrophoresed on an 5% polyacrylamide denaturing gel (H: Highcontent, L: Low content of glycitein seed content.
Mentions: Using subpopulation of 40 RILs with data from each plotreplicate, three chromosomal region on two different molecularlinkage groups were found to contain quantitative trait loci(QTL) for seed isoflavone content (Table 1). A regionon linkage group B1 identified by the microsatellite markerSatt251 was significantly (P = 0.0001, R2 = 49%) associatedwith glycitein content (Figure 2). The linked markersSatt197 and Satt415 were also significantly associated withglycitein content. The interval containing the QTL spanning about10 cM had LOD score of 10.6 and explained about 50% of totalvariation in glycitein content. The region derived the beneficialallele from Essex.

Bottom Line: Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect.Four genomic regions were found to be significantly associated with the isoflavone content of soybean seeds across both locations and years.In addition, tightly linked markers can be used in map based cloning of genes associated with isoflavone content.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Soy products contain isoflavones (genistein, daidzein, and glycitein) that display biological effects when ingested by humans and animals, these effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect. Our objective was to identify loci that underlie isoflavone content in soybean seeds. The study involved 100 recombinant inbred lines (RIL) from the cross of 'Essex' by 'Forrest,' two cultivars that contrast for isoflavone content. Isoflavone content of seeds from each RIL was determined by high performance liquid chromatography (HPLC). The distribution of isoflavone content was continuous and unimodal. The heritability estimates on a line mean basis were 79% for daidzein, 22% for genistein, and 88% for glycitein. Isoflavone content of soybean seeds was compared against 150 polymorphic DNA markers in a one-way analysis of variance. Four genomic regions were found to be significantly associated with the isoflavone content of soybean seeds across both locations and years. Molecular linkage group B1 contained a major QTL underlying glycitein content (P = 0.0001, R(2) = 50.2%), linkage group N contained a QTL for glycitein (P = 0.0033, R(2) = 11.1%) and a QTL for daidzein (P = 0.0023, R(2) = 10.3%) and linkage group A1 contained a QTL for daidzein (P = 0.0081, R(2) = 9.6%). Selection for these chromosomal regions in a marker assisted selection program will allow for the manipulation of amounts and profiles of isoflavones (genistein, daidzein, and glycitein) content of soybean seeds. In addition, tightly linked markers can be used in map based cloning of genes associated with isoflavone content.

No MeSH data available.