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Genomic Regions That Underlie Soybean Seed Isoflavone Content.

Meksem K, Njiti VN, Banz WJ, Iqbal MJ, Kassem MM, Hyten DL, Yuang J, Winters TA, Lightfoot DA - J. Biomed. Biotechnol. (2001)

Bottom Line: Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect.Four genomic regions were found to be significantly associated with the isoflavone content of soybean seeds across both locations and years.In addition, tightly linked markers can be used in map based cloning of genes associated with isoflavone content.

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ABSTRACT
Soy products contain isoflavones (genistein, daidzein, and glycitein) that display biological effects when ingested by humans and animals, these effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect. Our objective was to identify loci that underlie isoflavone content in soybean seeds. The study involved 100 recombinant inbred lines (RIL) from the cross of 'Essex' by 'Forrest,' two cultivars that contrast for isoflavone content. Isoflavone content of seeds from each RIL was determined by high performance liquid chromatography (HPLC). The distribution of isoflavone content was continuous and unimodal. The heritability estimates on a line mean basis were 79% for daidzein, 22% for genistein, and 88% for glycitein. Isoflavone content of soybean seeds was compared against 150 polymorphic DNA markers in a one-way analysis of variance. Four genomic regions were found to be significantly associated with the isoflavone content of soybean seeds across both locations and years. Molecular linkage group B1 contained a major QTL underlying glycitein content (P = 0.0001, R(2) = 50.2%), linkage group N contained a QTL for glycitein (P = 0.0033, R(2) = 11.1%) and a QTL for daidzein (P = 0.0023, R(2) = 10.3%) and linkage group A1 contained a QTL for daidzein (P = 0.0081, R(2) = 9.6%). Selection for these chromosomal regions in a marker assisted selection program will allow for the manipulation of amounts and profiles of isoflavones (genistein, daidzein, and glycitein) content of soybean seeds. In addition, tightly linked markers can be used in map based cloning of genes associated with isoflavone content.

No MeSH data available.


Frequency distributions of the mean isoflavone contentacross two locations among 96 recombinant inbred lines from across between cultivars Essex and Forrest. The mean isoflavonecontent for each parent is shown. A, daidzein content. B,genistein content. C, glycitein content. D, Total isoflavone. Theisoflavone content is calculated in μg/g of dry weight ofsoybean seed.
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Figure 1: Frequency distributions of the mean isoflavone contentacross two locations among 96 recombinant inbred lines from across between cultivars Essex and Forrest. The mean isoflavonecontent for each parent is shown. A, daidzein content. B,genistein content. C, glycitein content. D, Total isoflavone. Theisoflavone content is calculated in μg/g of dry weight ofsoybean seed.

Mentions: When all 100 RILs were pooled, the frequency distribution ofdaidzein, genistein, and glycitein all significantly departedfrom normality (Figure 1). Daidzein showed a peakeddistribution that was also skewed toward Essex, genistein had apeaked distribution that was not significantly skewed, glyciteinshowed a flattened distribution that was not significantlyskewed. All distributions were continuous and uni-model. There wastransgressive segregation for each isoflavone and totalisoflavone content. Recombinant inbred lines showed transgressivesegregation for high daidzein and genistein contents and for bothlow and high glycitein content. The group of RILs with highertotal isoflavone content than Forrest were significantly greaterthan Forrest when considered as a group.


Genomic Regions That Underlie Soybean Seed Isoflavone Content.

Meksem K, Njiti VN, Banz WJ, Iqbal MJ, Kassem MM, Hyten DL, Yuang J, Winters TA, Lightfoot DA - J. Biomed. Biotechnol. (2001)

Frequency distributions of the mean isoflavone contentacross two locations among 96 recombinant inbred lines from across between cultivars Essex and Forrest. The mean isoflavonecontent for each parent is shown. A, daidzein content. B,genistein content. C, glycitein content. D, Total isoflavone. Theisoflavone content is calculated in μg/g of dry weight ofsoybean seed.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC79679&req=5

Figure 1: Frequency distributions of the mean isoflavone contentacross two locations among 96 recombinant inbred lines from across between cultivars Essex and Forrest. The mean isoflavonecontent for each parent is shown. A, daidzein content. B,genistein content. C, glycitein content. D, Total isoflavone. Theisoflavone content is calculated in μg/g of dry weight ofsoybean seed.
Mentions: When all 100 RILs were pooled, the frequency distribution ofdaidzein, genistein, and glycitein all significantly departedfrom normality (Figure 1). Daidzein showed a peakeddistribution that was also skewed toward Essex, genistein had apeaked distribution that was not significantly skewed, glyciteinshowed a flattened distribution that was not significantlyskewed. All distributions were continuous and uni-model. There wastransgressive segregation for each isoflavone and totalisoflavone content. Recombinant inbred lines showed transgressivesegregation for high daidzein and genistein contents and for bothlow and high glycitein content. The group of RILs with highertotal isoflavone content than Forrest were significantly greaterthan Forrest when considered as a group.

Bottom Line: Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect.Four genomic regions were found to be significantly associated with the isoflavone content of soybean seeds across both locations and years.In addition, tightly linked markers can be used in map based cloning of genes associated with isoflavone content.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Soy products contain isoflavones (genistein, daidzein, and glycitein) that display biological effects when ingested by humans and animals, these effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect. Our objective was to identify loci that underlie isoflavone content in soybean seeds. The study involved 100 recombinant inbred lines (RIL) from the cross of 'Essex' by 'Forrest,' two cultivars that contrast for isoflavone content. Isoflavone content of seeds from each RIL was determined by high performance liquid chromatography (HPLC). The distribution of isoflavone content was continuous and unimodal. The heritability estimates on a line mean basis were 79% for daidzein, 22% for genistein, and 88% for glycitein. Isoflavone content of soybean seeds was compared against 150 polymorphic DNA markers in a one-way analysis of variance. Four genomic regions were found to be significantly associated with the isoflavone content of soybean seeds across both locations and years. Molecular linkage group B1 contained a major QTL underlying glycitein content (P = 0.0001, R(2) = 50.2%), linkage group N contained a QTL for glycitein (P = 0.0033, R(2) = 11.1%) and a QTL for daidzein (P = 0.0023, R(2) = 10.3%) and linkage group A1 contained a QTL for daidzein (P = 0.0081, R(2) = 9.6%). Selection for these chromosomal regions in a marker assisted selection program will allow for the manipulation of amounts and profiles of isoflavones (genistein, daidzein, and glycitein) content of soybean seeds. In addition, tightly linked markers can be used in map based cloning of genes associated with isoflavone content.

No MeSH data available.