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The Antinociceptive Effect of Light-Emitting Diode Irradiation on Incised Wounds Is Correlated with Changes in Cyclooxygenase 2 Activity, Prostaglandin E2, and Proinflammatory Cytokines

View Article: PubMed Central - PubMed

ABSTRACT

Background. Light-emitting diode (LED) phototherapy has been reported to relieve pain and enhance tissue repair through several mechanisms. However, the analgesic effect of LED on incised wounds has never been examined. Objectives. We examined the analgesic effect of LED therapy on incision pain and the changes in cyclooxygenase 2 (COX-2), prostaglandin E2 (PGE2), and the proinflammatory cytokines interleukin 6 (IL-6), IL-1β, and tumor necrosis factor α (TNF-α). Methods. Rats received LED therapy on incised skin 6 days before incision (L-I group) or 6 days after incision (I-L group) or from 3 days before incision to 3 days after incision (L-I-L group). Behavioral tests and analysis of skin tissue were performed after LED therapy. Results. LED therapy attenuated the decrease in thermal withdrawal latency in all the irradiated groups and the decrease in the mechanical withdrawal threshold in the L-I group only. The expression levels of COX-2, PGE2, and IL-6 were significantly decreased in the three LED-treated groups, whereas IL-1β and TNF-α were significantly decreased only in the L-I group compared with their levels in the I groups (p < 0.05). Conclusions. LED therapy provides an analgesic effect and modifies the expression of COX-2, PGE2, and proinflammatory cytokines in incised skin.

No MeSH data available.


Related in: MedlinePlus

Mean withdrawal latency in the six groups (n = 6 each group) 6 days after LED irradiation performed before or after skin incision. C indicates LED irradiation for 6 days before or after sham skin incision on the paw contralateral to the LED-irradiated incised paw; I-3 h, I-3 d, and I-6 d indicate incision only groups that underwent behavioral testing 3 hours, 3 days, and 6 days after skin incision; L-I indicates LED irradiation for 6 days before skin incision; I-L indicates LED irradiation for 6 days after skin incision; L-I-L indicates LED irradiation from 3 days before skin incision to 3 days after skin incision. ∗p < 0.05 compared with the baseline value in each group. The values represent the means ± SD.
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fig1: Mean withdrawal latency in the six groups (n = 6 each group) 6 days after LED irradiation performed before or after skin incision. C indicates LED irradiation for 6 days before or after sham skin incision on the paw contralateral to the LED-irradiated incised paw; I-3 h, I-3 d, and I-6 d indicate incision only groups that underwent behavioral testing 3 hours, 3 days, and 6 days after skin incision; L-I indicates LED irradiation for 6 days before skin incision; I-L indicates LED irradiation for 6 days after skin incision; L-I-L indicates LED irradiation from 3 days before skin incision to 3 days after skin incision. ∗p < 0.05 compared with the baseline value in each group. The values represent the means ± SD.

Mentions: The test of the analgesic effect of LED therapy on incision pain produced the following results. After the skin was incised, the thermal withdrawal latency was significantly decreased compared with the baseline value in three I groups (p < 0.05), but not in the three LED-treated groups (Figures 1(a), 1(b), and 1(c)). Significantly decreased mechanical withdrawal thresholds compared with the baseline value were noted after skin incision in the L-I-L and I-L groups but not in the L-I group (p < 0.05) (Figures 2(a), 2(b), and 2(c)).


The Antinociceptive Effect of Light-Emitting Diode Irradiation on Incised Wounds Is Correlated with Changes in Cyclooxygenase 2 Activity, Prostaglandin E2, and Proinflammatory Cytokines
Mean withdrawal latency in the six groups (n = 6 each group) 6 days after LED irradiation performed before or after skin incision. C indicates LED irradiation for 6 days before or after sham skin incision on the paw contralateral to the LED-irradiated incised paw; I-3 h, I-3 d, and I-6 d indicate incision only groups that underwent behavioral testing 3 hours, 3 days, and 6 days after skin incision; L-I indicates LED irradiation for 6 days before skin incision; I-L indicates LED irradiation for 6 days after skin incision; L-I-L indicates LED irradiation from 3 days before skin incision to 3 days after skin incision. ∗p < 0.05 compared with the baseline value in each group. The values represent the means ± SD.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5392408&req=5

fig1: Mean withdrawal latency in the six groups (n = 6 each group) 6 days after LED irradiation performed before or after skin incision. C indicates LED irradiation for 6 days before or after sham skin incision on the paw contralateral to the LED-irradiated incised paw; I-3 h, I-3 d, and I-6 d indicate incision only groups that underwent behavioral testing 3 hours, 3 days, and 6 days after skin incision; L-I indicates LED irradiation for 6 days before skin incision; I-L indicates LED irradiation for 6 days after skin incision; L-I-L indicates LED irradiation from 3 days before skin incision to 3 days after skin incision. ∗p < 0.05 compared with the baseline value in each group. The values represent the means ± SD.
Mentions: The test of the analgesic effect of LED therapy on incision pain produced the following results. After the skin was incised, the thermal withdrawal latency was significantly decreased compared with the baseline value in three I groups (p < 0.05), but not in the three LED-treated groups (Figures 1(a), 1(b), and 1(c)). Significantly decreased mechanical withdrawal thresholds compared with the baseline value were noted after skin incision in the L-I-L and I-L groups but not in the L-I group (p < 0.05) (Figures 2(a), 2(b), and 2(c)).

View Article: PubMed Central - PubMed

ABSTRACT

Background. Light-emitting diode (LED) phototherapy has been reported to relieve pain and enhance tissue repair through several mechanisms. However, the analgesic effect of LED on incised wounds has never been examined. Objectives. We examined the analgesic effect of LED therapy on incision pain and the changes in cyclooxygenase 2 (COX-2), prostaglandin E2 (PGE2), and the proinflammatory cytokines interleukin 6 (IL-6), IL-1&beta;, and tumor necrosis factor &alpha; (TNF-&alpha;). Methods. Rats received LED therapy on incised skin 6 days before incision (L-I group) or 6 days after incision (I-L group) or from 3 days before incision to 3 days after incision (L-I-L group). Behavioral tests and analysis of skin tissue were performed after LED therapy. Results. LED therapy attenuated the decrease in thermal withdrawal latency in all the irradiated groups and the decrease in the mechanical withdrawal threshold in the L-I group only. The expression levels of COX-2, PGE2, and IL-6 were significantly decreased in the three LED-treated groups, whereas IL-1&beta; and TNF-&alpha; were significantly decreased only in the L-I group compared with their levels in the I groups (p &lt; 0.05). Conclusions. LED therapy provides an analgesic effect and modifies the expression of COX-2, PGE2, and proinflammatory cytokines in incised skin.

No MeSH data available.


Related in: MedlinePlus