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Tuber indicum shapes the microbial communities of ectomycorhizosphere soil and ectomycorrhizae of an indigenous tree ( Pinus armandii )

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ABSTRACT

The aim of this study was to investigate the effect of an ectomycorrhizal fungus (Tuber indicum) on the diversity of microbial communities associated with an indigenous tree, Pinus armandii, and the microbial communities in the surrounding ectomycorhizosphere soil. High-throughput sequencing was used to analyze the richness of microbial communities in the roots or rhizosphere of treatments with or without ectomycorrhizae. The results indicated that the bacterial diversity of ectomycorhizosphere soil was significantly lower compared with the control soil. Presumably, the dominance of truffle mycelia in ectomycorhizosphere soil (80.91%) and ectomycorrhizae (97.64%) was the main factor that resulted in lower diversity and abundance of endophytic pathogenic fungi, including Fusarium, Monographella, Ustilago and Rhizopus and other competitive mycorrhizal fungi, such as Amanita, Lactarius and Boletus. Bacterial genera Reyranena, Rhizomicrobium, Nordella, Pseudomonas and fungal genera, Cuphophyllus, Leucangium, Histoplasma were significantly more abundant in ectomycorrhizosphere soil and ectomycorrhizae. Hierarchical cluster analysis of the similarities between rhizosphere and ectomycorrhizosphere soil based on the soil properties differed significantly, indicating the mycorrhizal synthesis may have a feedback effect on soil properties. Meanwhile, some soil properties were significantly correlated with bacterial and fungal diversity in the rhizosphere or root tips. Overall, this work illustrates the interactive network that exists among ectomycorrhizal fungi, soil properties and microbial communities associated with the host plant and furthers our understanding of the ecology and cultivation of T. indicum.

No MeSH data available.


Hierarchical cluster analysis of the similarities among different samples based on the soil properties using the the between-groups linkage method in SPSS.CK.S, rhizosphere soil; ECM.S, ectomycorrhizosphere soil.
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pone.0175720.g002: Hierarchical cluster analysis of the similarities among different samples based on the soil properties using the the between-groups linkage method in SPSS.CK.S, rhizosphere soil; ECM.S, ectomycorrhizosphere soil.

Mentions: A cluster analysis dendrogram of the soil property similarities between different samples show two main clusters (Fig 2). The first cluster was rhizosphere soil and the second was ectomycorrhizosphere soil. These two treatments showed significant variability in soil properties, such as the organic matter, total nitrogen, available phosphorus, available calcium and available magnesium, indicating a feedback effect of ectomycorrhiza on the surrounding soil.


Tuber indicum shapes the microbial communities of ectomycorhizosphere soil and ectomycorrhizae of an indigenous tree ( Pinus armandii )
Hierarchical cluster analysis of the similarities among different samples based on the soil properties using the the between-groups linkage method in SPSS.CK.S, rhizosphere soil; ECM.S, ectomycorrhizosphere soil.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5391931&req=5

pone.0175720.g002: Hierarchical cluster analysis of the similarities among different samples based on the soil properties using the the between-groups linkage method in SPSS.CK.S, rhizosphere soil; ECM.S, ectomycorrhizosphere soil.
Mentions: A cluster analysis dendrogram of the soil property similarities between different samples show two main clusters (Fig 2). The first cluster was rhizosphere soil and the second was ectomycorrhizosphere soil. These two treatments showed significant variability in soil properties, such as the organic matter, total nitrogen, available phosphorus, available calcium and available magnesium, indicating a feedback effect of ectomycorrhiza on the surrounding soil.

View Article: PubMed Central - PubMed

ABSTRACT

The aim of this study was to investigate the effect of an ectomycorrhizal fungus (Tuber indicum) on the diversity of microbial communities associated with an indigenous tree, Pinus armandii, and the microbial communities in the surrounding ectomycorhizosphere soil. High-throughput sequencing was used to analyze the richness of microbial communities in the roots or rhizosphere of treatments with or without ectomycorrhizae. The results indicated that the bacterial diversity of ectomycorhizosphere soil was significantly lower compared with the control soil. Presumably, the dominance of truffle mycelia in ectomycorhizosphere soil (80.91%) and ectomycorrhizae (97.64%) was the main factor that resulted in lower diversity and abundance of endophytic pathogenic fungi, including Fusarium, Monographella, Ustilago and Rhizopus and other competitive mycorrhizal fungi, such as Amanita, Lactarius and Boletus. Bacterial genera Reyranena, Rhizomicrobium, Nordella, Pseudomonas and fungal genera, Cuphophyllus, Leucangium, Histoplasma were significantly more abundant in ectomycorrhizosphere soil and ectomycorrhizae. Hierarchical cluster analysis of the similarities between rhizosphere and ectomycorrhizosphere soil based on the soil properties differed significantly, indicating the mycorrhizal synthesis may have a feedback effect on soil properties. Meanwhile, some soil properties were significantly correlated with bacterial and fungal diversity in the rhizosphere or root tips. Overall, this work illustrates the interactive network that exists among ectomycorrhizal fungi, soil properties and microbial communities associated with the host plant and furthers our understanding of the ecology and cultivation of T. indicum.

No MeSH data available.