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In situ detection of Chlamydia pneumoniae, C. trachomatis , and cytokines among cardiovascular diseased patients from the Amazon region of Brazil

View Article: PubMed Central - PubMed

ABSTRACT

Background: Chronic coronary artery disease has been associated, as a consequence of the local inflammatory reaction with previous or persistent infection with Chlamydia pneumoniae, which led to the investigation of the association of cardiovascular disease and previous infection with C. trachomatis and the role of cytokine profile (in situ) markers in the vascular system tissues.

Methods: Sixty-nine biopsies were collected for immunohistochemical analysis for the presence of IL-6, IL-8, TNF-α, IFN-γ, TGF-β, and IL-10, in 16 fragments from atheromatous plaques, 32 aorta fragments, and 21 valve fragments, using a tissue microarray technique for paraffin embedded tissues.

Results: Most patients undergoing revascularization surgery were men >50 years, while those undergoing valve replacement were mostly women <50 years. TNF-α was the most prevalent marker, detected in 91.7% (55/60) of the samples. The mean percent area stained was greater in patients infected with C. pneumoniae (3.81% vs 1.92%; p=0.0115) and specifically in the aorta (4.83% vs 2.25%; p=0.0025); C. trachomatis infection was higher in valves, and C. pneumoniae in plaques, both without statistical significance. There was no significant difference in the cytokine staining profile between patients previously infected with both species and uninfected patients.

Conclusion: Although there was no difference in the cytokine profile between patients previously infected with both species of Chlamydia, and uninfected patients, the presence of the bacteria antigens in the three biological specimens indicates it is important to focus on the role of C. trachomatis. It is necessary to improve the understanding of the natural history of chronic coronary artery disease and the clinical history of the patients and cytokine dynamics in cardiac disease in the presence or absence of infectious agents.

No MeSH data available.


Related in: MedlinePlus

(A) Mean and standard deviation of the average percentage counts of the stained areas for C.p and C.t. Mann–Whitney. (B) Comparison of the mean values of the average percentage counts of the stained areas for C.p and C.t, according to the histological sections. Mann–Whitney.Abbreviations:C.p, Chlamydia pneumoniae; C.t, Chlamydia trachomatis; +, positive; −, negative; V_C.p+, C. pneumoniae positives in valves; V_C.t+, C. trachomatis positives in valves; P_C.p+, C. pneumoniae positives in atheromatous plaques; AO_C.p+, C. pneumoniae positives in aorta fragments; AO_C.t+, C. trachomatis positives in aorta fragments.
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f2-idr-10-109: (A) Mean and standard deviation of the average percentage counts of the stained areas for C.p and C.t. Mann–Whitney. (B) Comparison of the mean values of the average percentage counts of the stained areas for C.p and C.t, according to the histological sections. Mann–Whitney.Abbreviations:C.p, Chlamydia pneumoniae; C.t, Chlamydia trachomatis; +, positive; −, negative; V_C.p+, C. pneumoniae positives in valves; V_C.t+, C. trachomatis positives in valves; P_C.p+, C. pneumoniae positives in atheromatous plaques; AO_C.p+, C. pneumoniae positives in aorta fragments; AO_C.t+, C. trachomatis positives in aorta fragments.

Mentions: A significant difference was observed in the overall percent area stained, which was almost twice of that for C. pneumoniae compared to C. trachomatis (3.814 vs 1.9818; Figure 2A). The presence of C. trachomatis was higher in the valves (2.474 vs 1.800) and greater for C. pneumoniae in the plaques (3.783 vs 0.702), but without statistical significance in both situations. The aorta fragments, however, showed a significantly higher difference in the stained areas for C. pneumoniae (4.830 vs 2.247; Figure 2B).


In situ detection of Chlamydia pneumoniae, C. trachomatis , and cytokines among cardiovascular diseased patients from the Amazon region of Brazil
(A) Mean and standard deviation of the average percentage counts of the stained areas for C.p and C.t. Mann–Whitney. (B) Comparison of the mean values of the average percentage counts of the stained areas for C.p and C.t, according to the histological sections. Mann–Whitney.Abbreviations:C.p, Chlamydia pneumoniae; C.t, Chlamydia trachomatis; +, positive; −, negative; V_C.p+, C. pneumoniae positives in valves; V_C.t+, C. trachomatis positives in valves; P_C.p+, C. pneumoniae positives in atheromatous plaques; AO_C.p+, C. pneumoniae positives in aorta fragments; AO_C.t+, C. trachomatis positives in aorta fragments.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5391863&req=5

f2-idr-10-109: (A) Mean and standard deviation of the average percentage counts of the stained areas for C.p and C.t. Mann–Whitney. (B) Comparison of the mean values of the average percentage counts of the stained areas for C.p and C.t, according to the histological sections. Mann–Whitney.Abbreviations:C.p, Chlamydia pneumoniae; C.t, Chlamydia trachomatis; +, positive; −, negative; V_C.p+, C. pneumoniae positives in valves; V_C.t+, C. trachomatis positives in valves; P_C.p+, C. pneumoniae positives in atheromatous plaques; AO_C.p+, C. pneumoniae positives in aorta fragments; AO_C.t+, C. trachomatis positives in aorta fragments.
Mentions: A significant difference was observed in the overall percent area stained, which was almost twice of that for C. pneumoniae compared to C. trachomatis (3.814 vs 1.9818; Figure 2A). The presence of C. trachomatis was higher in the valves (2.474 vs 1.800) and greater for C. pneumoniae in the plaques (3.783 vs 0.702), but without statistical significance in both situations. The aorta fragments, however, showed a significantly higher difference in the stained areas for C. pneumoniae (4.830 vs 2.247; Figure 2B).

View Article: PubMed Central - PubMed

ABSTRACT

Background: Chronic coronary artery disease has been associated, as a consequence of the local inflammatory reaction with previous or persistent infection with Chlamydia pneumoniae, which led to the investigation of the association of cardiovascular disease and previous infection with C. trachomatis and the role of cytokine profile (in situ) markers in the vascular system tissues.

Methods: Sixty-nine biopsies were collected for immunohistochemical analysis for the presence of IL-6, IL-8, TNF-α, IFN-γ, TGF-β, and IL-10, in 16 fragments from atheromatous plaques, 32 aorta fragments, and 21 valve fragments, using a tissue microarray technique for paraffin embedded tissues.

Results: Most patients undergoing revascularization surgery were men >50 years, while those undergoing valve replacement were mostly women <50 years. TNF-α was the most prevalent marker, detected in 91.7% (55/60) of the samples. The mean percent area stained was greater in patients infected with C. pneumoniae (3.81% vs 1.92%; p=0.0115) and specifically in the aorta (4.83% vs 2.25%; p=0.0025); C. trachomatis infection was higher in valves, and C. pneumoniae in plaques, both without statistical significance. There was no significant difference in the cytokine staining profile between patients previously infected with both species and uninfected patients.

Conclusion: Although there was no difference in the cytokine profile between patients previously infected with both species of Chlamydia, and uninfected patients, the presence of the bacteria antigens in the three biological specimens indicates it is important to focus on the role of C. trachomatis. It is necessary to improve the understanding of the natural history of chronic coronary artery disease and the clinical history of the patients and cytokine dynamics in cardiac disease in the presence or absence of infectious agents.

No MeSH data available.


Related in: MedlinePlus