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EGFR-targeted delivery of DOX-loaded Fe 3 O 4 @ polydopamine multifunctional nanocomposites for MRI and antitumor chemo-photothermal therapy

View Article: PubMed Central - PubMed

ABSTRACT

Multifunctional nanocomposites that have multiple therapeutic functions together with real-time imaging capabilities have attracted intensive concerns in the diagnosis and treatment of cancer. This study developed epidermal growth factor receptor (EGFR) antibody-directed polydopamine-coated Fe3O4 nanoparticles (Fe3O4@PDA NPs) for magnetic resonance imaging and antitumor chemo-photothermal therapy. The synthesized Fe3O4@PDA-PEG-EGFR-DOX NPs revealed high storage capacity for doxorubicin (DOX) and high photothermal conversion efficiency. The cell viability assay of Fe3O4@PDA-PEG-EGFR NPs indicated that Fe3O4@ PDA-PEG-EGFR NPs had no cell cytotoxicity. However, Fe3O4@PDA-PEG-EGFR-DOX NPs could significantly decrease cell viability (~5% of remaining cell viability) because of both photothermal ablation and near-infrared light-triggered DOX release. Meanwhile, the EGFR-targeted Fe3O4@PDA-PEG-EGFR-DOX NPs significantly inhibited the growth of tumors, showing a prominent in vivo synergistic antitumor effect. This study demonstrated the potential of using Fe3O4@PDA NPs for combined cancer chemo-photothermal therapy with increased efficacy.

No MeSH data available.


Photothermal properties of Fe3O4@PDA-PEG-EGFR NPs.Notes: (A) Temperature elevation of Fe3O4@PDA-PEG-EGFR NP solution at various concentrations irradiated with an 808 nm NIR laser (0.6 W/cm2, 6 min) measured every 1 min using a thermometer. (B) Temperature elevation of Fe3O4@PDA-PEG-EGFR NPs over five laser on/off cycles under NIR laser irradiation. Red arrows under the curve represent laser-turn on, red arrows on the curve represent laser-turn off.Abbreviations: NIR, near-infrared; PDA, polydopamine; PEG, polyethylene glycol; NP, nanoparticle.
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f4-ijn-12-2899: Photothermal properties of Fe3O4@PDA-PEG-EGFR NPs.Notes: (A) Temperature elevation of Fe3O4@PDA-PEG-EGFR NP solution at various concentrations irradiated with an 808 nm NIR laser (0.6 W/cm2, 6 min) measured every 1 min using a thermometer. (B) Temperature elevation of Fe3O4@PDA-PEG-EGFR NPs over five laser on/off cycles under NIR laser irradiation. Red arrows under the curve represent laser-turn on, red arrows on the curve represent laser-turn off.Abbreviations: NIR, near-infrared; PDA, polydopamine; PEG, polyethylene glycol; NP, nanoparticle.

Mentions: The photothermal conversion capability of Fe3O4@PDA-PEG-EGFR NPs was evaluated by irradiating Fe3O4@PDA-PEG-EGFR aqueous solution with an 808 nm NIR laser at 0.6 W/cm2. As shown in Figure 4A, the temperature strikingly rised with increasing concentration of Fe3O4@PDA-PEG-EGFR NPs, following a time- and concentration-dependent manner. In particular, at an Fe3O4@PDA-PEG-EGFR NPs concentration of 150 µg/mL, the solution temperature could reach 50°C within 6 min irradiation, which is the temperature required to kill cancer cells.26 The thermal stability of Fe3O4@PDA-PEG-EGFR NPs was assessed by NIR laser irradiations at 0.6 W/cm2 for 6 min, followed by natural cooling of temperature to room temperature for five cycles. After five cycles of laser irradiation, there was no noticeable attenuation in the thermal conversion efficiency of the Fe3O4@PDA-PEG-EGFR NPs (Figure 4B). The remarkable photothermal conversion efficiency and thermal stability indicated that the Fe3O4@PDA-PEG-EGFR NPs could be used as an excellent candidate for PTT applications.


EGFR-targeted delivery of DOX-loaded Fe 3 O 4 @ polydopamine multifunctional nanocomposites for MRI and antitumor chemo-photothermal therapy
Photothermal properties of Fe3O4@PDA-PEG-EGFR NPs.Notes: (A) Temperature elevation of Fe3O4@PDA-PEG-EGFR NP solution at various concentrations irradiated with an 808 nm NIR laser (0.6 W/cm2, 6 min) measured every 1 min using a thermometer. (B) Temperature elevation of Fe3O4@PDA-PEG-EGFR NPs over five laser on/off cycles under NIR laser irradiation. Red arrows under the curve represent laser-turn on, red arrows on the curve represent laser-turn off.Abbreviations: NIR, near-infrared; PDA, polydopamine; PEG, polyethylene glycol; NP, nanoparticle.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC5391832&req=5

f4-ijn-12-2899: Photothermal properties of Fe3O4@PDA-PEG-EGFR NPs.Notes: (A) Temperature elevation of Fe3O4@PDA-PEG-EGFR NP solution at various concentrations irradiated with an 808 nm NIR laser (0.6 W/cm2, 6 min) measured every 1 min using a thermometer. (B) Temperature elevation of Fe3O4@PDA-PEG-EGFR NPs over five laser on/off cycles under NIR laser irradiation. Red arrows under the curve represent laser-turn on, red arrows on the curve represent laser-turn off.Abbreviations: NIR, near-infrared; PDA, polydopamine; PEG, polyethylene glycol; NP, nanoparticle.
Mentions: The photothermal conversion capability of Fe3O4@PDA-PEG-EGFR NPs was evaluated by irradiating Fe3O4@PDA-PEG-EGFR aqueous solution with an 808 nm NIR laser at 0.6 W/cm2. As shown in Figure 4A, the temperature strikingly rised with increasing concentration of Fe3O4@PDA-PEG-EGFR NPs, following a time- and concentration-dependent manner. In particular, at an Fe3O4@PDA-PEG-EGFR NPs concentration of 150 µg/mL, the solution temperature could reach 50°C within 6 min irradiation, which is the temperature required to kill cancer cells.26 The thermal stability of Fe3O4@PDA-PEG-EGFR NPs was assessed by NIR laser irradiations at 0.6 W/cm2 for 6 min, followed by natural cooling of temperature to room temperature for five cycles. After five cycles of laser irradiation, there was no noticeable attenuation in the thermal conversion efficiency of the Fe3O4@PDA-PEG-EGFR NPs (Figure 4B). The remarkable photothermal conversion efficiency and thermal stability indicated that the Fe3O4@PDA-PEG-EGFR NPs could be used as an excellent candidate for PTT applications.

View Article: PubMed Central - PubMed

ABSTRACT

Multifunctional nanocomposites that have multiple therapeutic functions together with real-time imaging capabilities have attracted intensive concerns in the diagnosis and treatment of cancer. This study developed epidermal growth factor receptor (EGFR) antibody-directed polydopamine-coated Fe3O4 nanoparticles (Fe3O4@PDA NPs) for magnetic resonance imaging and antitumor chemo-photothermal therapy. The synthesized Fe3O4@PDA-PEG-EGFR-DOX NPs revealed high storage capacity for doxorubicin (DOX) and high photothermal conversion efficiency. The cell viability assay of Fe3O4@PDA-PEG-EGFR NPs indicated that Fe3O4@ PDA-PEG-EGFR NPs had no cell cytotoxicity. However, Fe3O4@PDA-PEG-EGFR-DOX NPs could significantly decrease cell viability (~5% of remaining cell viability) because of both photothermal ablation and near-infrared light-triggered DOX release. Meanwhile, the EGFR-targeted Fe3O4@PDA-PEG-EGFR-DOX NPs significantly inhibited the growth of tumors, showing a prominent in vivo synergistic antitumor effect. This study demonstrated the potential of using Fe3O4@PDA NPs for combined cancer chemo-photothermal therapy with increased efficacy.

No MeSH data available.