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Engineered microbial biosensors based on bacterial two-component systems as synthetic biotechnology platforms in bioremediation and biorefinery

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ABSTRACT

Two-component regulatory systems (TCRSs) mediate cellular response by coupling sensing and regulatory mechanisms. TCRSs are comprised of a histidine kinase (HK), which serves as a sensor, and a response regulator, which regulates expression of the effector gene after being phosphorylated by HK. Using these attributes, bacterial TCRSs can be engineered to design microbial systems for different applications. This review focuses on the current advances in TCRS-based biosensors and on the design of microbial systems for bioremediation and their potential application in biorefinery.

No MeSH data available.


Application of different cell surface display technologies in A antibody production, B peptide library screening, C biosensors, D biocatalysts, E bio-adsorption, and F vaccine development
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Fig1: Application of different cell surface display technologies in A antibody production, B peptide library screening, C biosensors, D biocatalysts, E bio-adsorption, and F vaccine development

Mentions: The central principle of bioremediation is that microorganisms are able to produce energy they need to grow and reproduce by degrading hazardous contaminants. In some cases, bioremediation occurs spontaneously because the essential materials required for bacterial growth are naturally present at the contaminated sites. More often, bioremediation requires an engineered bacterial system to accelerate the tailor-made biodegradation of organic compounds or bio-adsorption of inorganic elements as we desired [2, 3]. It is also needed to further optimize the environmental conditions, in which the microorganisms carry out the detoxification reactions by employing several engineered microorganism systems such as cell surface display- and secretion-based strategies to remediate the contaminated environment. Cell surface display technologies have widely been used in both pharmaceutical and bioremediation applications such as live vaccine development, antibody production, peptide library screening, biosensors, bio-adsorption of organic and inorganic pollutants, and whole-cell biocatalysis (Fig. 1) [4–7].Fig. 1


Engineered microbial biosensors based on bacterial two-component systems as synthetic biotechnology platforms in bioremediation and biorefinery
Application of different cell surface display technologies in A antibody production, B peptide library screening, C biosensors, D biocatalysts, E bio-adsorption, and F vaccine development
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5391612&req=5

Fig1: Application of different cell surface display technologies in A antibody production, B peptide library screening, C biosensors, D biocatalysts, E bio-adsorption, and F vaccine development
Mentions: The central principle of bioremediation is that microorganisms are able to produce energy they need to grow and reproduce by degrading hazardous contaminants. In some cases, bioremediation occurs spontaneously because the essential materials required for bacterial growth are naturally present at the contaminated sites. More often, bioremediation requires an engineered bacterial system to accelerate the tailor-made biodegradation of organic compounds or bio-adsorption of inorganic elements as we desired [2, 3]. It is also needed to further optimize the environmental conditions, in which the microorganisms carry out the detoxification reactions by employing several engineered microorganism systems such as cell surface display- and secretion-based strategies to remediate the contaminated environment. Cell surface display technologies have widely been used in both pharmaceutical and bioremediation applications such as live vaccine development, antibody production, peptide library screening, biosensors, bio-adsorption of organic and inorganic pollutants, and whole-cell biocatalysis (Fig. 1) [4–7].Fig. 1

View Article: PubMed Central - PubMed

ABSTRACT

Two-component regulatory systems (TCRSs) mediate cellular response by coupling sensing and regulatory mechanisms. TCRSs are comprised of a histidine kinase (HK), which serves as a sensor, and a response regulator, which regulates expression of the effector gene after being phosphorylated by HK. Using these attributes, bacterial TCRSs can be engineered to design microbial systems for different applications. This review focuses on the current advances in TCRS-based biosensors and on the design of microbial systems for bioremediation and their potential application in biorefinery.

No MeSH data available.