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Transcriptomic analysis of the biosynthesis, recycling, and distribution of ascorbic acid during leaf development in tea plant ( Camellia sinensis (L.) O. Kuntze)

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ABSTRACT

Ascorbic acid (AsA), known as vitamin C, is an essential nutrient for humans and mainly absorbed from food. Tea plant (Camellia sinensis (L.) O. Kuntze) leaves can be a dietary source of AsA for humans. However, experimental evidence on the biosynthesis, recycling pathway and distribution of AsA during leaf development in tea plants is unclear. To gain insight into the mechanism and distribution of AsA in the tea plant leaf, we identified 18 related genes involved in AsA biosynthesis and recycling pathway based on the transcriptome database of tea plants. Tea plant leaves were used as samples at different developmental stages. AsA contens in tea plant leaves at three developmental stages were measured by reversed-phase high-performance liquid chromatography (RP-HPLC). The correlations between expression levels of these genes and AsA contents during the development of tea plant leaves were discussed. Results indicated that the l-galactose pathway might be the primary pathway of AsA biosynthesis in tea plant leaves. CsMDHAR and CsGGP might play a regulatory role in AsA accumulation in the leaves of three cultivars of tea plants. These findings may provide a further glimpse to improve the AsA accumulation in tea plants and the commercial quality of tea.

No MeSH data available.


Heatmap of the relative expression level of genes involved in AsA biosynthesis and recycling in tea plant.T1 was ‘Yunnanshilixiang’, T2 was ‘Changwansanhao’, T3 was ‘Ruchengmaoyecha’, and T4 was ‘Anjibaicha’.
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f6: Heatmap of the relative expression level of genes involved in AsA biosynthesis and recycling in tea plant.T1 was ‘Yunnanshilixiang’, T2 was ‘Changwansanhao’, T3 was ‘Ruchengmaoyecha’, and T4 was ‘Anjibaicha’.

Mentions: RNA sequencing (RNA-seq) data was extracted from transcriptome database in the four tea plant cultivars (Tea_T1, Tea_T2, Tea_T3, and Tea_T4)41. These tea plants were grown under non-stress conditions. The expression levels of genes involved in AsA biosynthesis and recycling were analyzed in another four other tea plants using RNA-seq data. RPKM values (Reads per kilobase per million mapped reads) were used to analyze the transcript levels of 18 genes, and a heatmap was obtained using HemI software (version1.0; http://hemi.biocuckoo.org/faq.php)42 (Fig. 6). CsAPX was expressed at the highest level (RPKM > 409) in Tea_T3. CsGGP showed a similar expression pattern in Tea_T1, Tea_T2, and Tea_T4. Both CsPMI and CsAO showed relatively low expression levels (RPKM > 2) in Tea_T3. CsMDHAR was highly expressed (RPKM > 291) in Tea_T1. In addition, CsGMP and CsGME, which participate in the AsA biosynthesis pathway were highly expressed in Tea_T1, Tea_T2, and Tea_T4. CsMDHAR and CsAPX were highly expressed in Tea_T1 and Tea_T4, whereas CsGalUR showed relatively low expression levels in the four tea plant cultivars.


Transcriptomic analysis of the biosynthesis, recycling, and distribution of ascorbic acid during leaf development in tea plant ( Camellia sinensis (L.) O. Kuntze)
Heatmap of the relative expression level of genes involved in AsA biosynthesis and recycling in tea plant.T1 was ‘Yunnanshilixiang’, T2 was ‘Changwansanhao’, T3 was ‘Ruchengmaoyecha’, and T4 was ‘Anjibaicha’.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5385563&req=5

f6: Heatmap of the relative expression level of genes involved in AsA biosynthesis and recycling in tea plant.T1 was ‘Yunnanshilixiang’, T2 was ‘Changwansanhao’, T3 was ‘Ruchengmaoyecha’, and T4 was ‘Anjibaicha’.
Mentions: RNA sequencing (RNA-seq) data was extracted from transcriptome database in the four tea plant cultivars (Tea_T1, Tea_T2, Tea_T3, and Tea_T4)41. These tea plants were grown under non-stress conditions. The expression levels of genes involved in AsA biosynthesis and recycling were analyzed in another four other tea plants using RNA-seq data. RPKM values (Reads per kilobase per million mapped reads) were used to analyze the transcript levels of 18 genes, and a heatmap was obtained using HemI software (version1.0; http://hemi.biocuckoo.org/faq.php)42 (Fig. 6). CsAPX was expressed at the highest level (RPKM > 409) in Tea_T3. CsGGP showed a similar expression pattern in Tea_T1, Tea_T2, and Tea_T4. Both CsPMI and CsAO showed relatively low expression levels (RPKM > 2) in Tea_T3. CsMDHAR was highly expressed (RPKM > 291) in Tea_T1. In addition, CsGMP and CsGME, which participate in the AsA biosynthesis pathway were highly expressed in Tea_T1, Tea_T2, and Tea_T4. CsMDHAR and CsAPX were highly expressed in Tea_T1 and Tea_T4, whereas CsGalUR showed relatively low expression levels in the four tea plant cultivars.

View Article: PubMed Central - PubMed

ABSTRACT

Ascorbic acid (AsA), known as vitamin C, is an essential nutrient for humans and mainly absorbed from food. Tea plant (Camellia sinensis (L.) O. Kuntze) leaves can be a dietary source of AsA for humans. However, experimental evidence on the biosynthesis, recycling pathway and distribution of AsA during leaf development in tea plants is unclear. To gain insight into the mechanism and distribution of AsA in the tea plant leaf, we identified 18 related genes involved in AsA biosynthesis and recycling pathway based on the transcriptome database of tea plants. Tea plant leaves were used as samples at different developmental stages. AsA contens in tea plant leaves at three developmental stages were measured by reversed-phase high-performance liquid chromatography (RP-HPLC). The correlations between expression levels of these genes and AsA contents during the development of tea plant leaves were discussed. Results indicated that the l-galactose pathway might be the primary pathway of AsA biosynthesis in tea plant leaves. CsMDHAR and CsGGP might play a regulatory role in AsA accumulation in the leaves of three cultivars of tea plants. These findings may provide a further glimpse to improve the AsA accumulation in tea plants and the commercial quality of tea.

No MeSH data available.