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Transcriptomic analysis of the biosynthesis, recycling, and distribution of ascorbic acid during leaf development in tea plant ( Camellia sinensis (L.) O. Kuntze)

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ABSTRACT

Ascorbic acid (AsA), known as vitamin C, is an essential nutrient for humans and mainly absorbed from food. Tea plant (Camellia sinensis (L.) O. Kuntze) leaves can be a dietary source of AsA for humans. However, experimental evidence on the biosynthesis, recycling pathway and distribution of AsA during leaf development in tea plants is unclear. To gain insight into the mechanism and distribution of AsA in the tea plant leaf, we identified 18 related genes involved in AsA biosynthesis and recycling pathway based on the transcriptome database of tea plants. Tea plant leaves were used as samples at different developmental stages. AsA contens in tea plant leaves at three developmental stages were measured by reversed-phase high-performance liquid chromatography (RP-HPLC). The correlations between expression levels of these genes and AsA contents during the development of tea plant leaves were discussed. Results indicated that the l-galactose pathway might be the primary pathway of AsA biosynthesis in tea plant leaves. CsMDHAR and CsGGP might play a regulatory role in AsA accumulation in the leaves of three cultivars of tea plants. These findings may provide a further glimpse to improve the AsA accumulation in tea plants and the commercial quality of tea.

No MeSH data available.


Expression level analyses of genes involved in AsA biosynthesis pathway in tea plant leaves.Genes involved in AsA biosynthesis pathway (A) Phosphoglucose isomerase (CsPGI1), (B) (CsPGI2), (C) phosphomannose isomerase (CsPMI), (D) phosphomannose mutase (CsPMM), (E) GDP-d-mannose pyrophosphorylase (CsGMP), (F) GDP-d-mannose-3′,5′-epimerase (CsGME), (G) GDP-l-galactose phosphorylase (CsGGP), (H) l-galactose-1-P phosphatase (CsGPP), (I) l-galactose dehydrogenase (CsGalDH), (J) l-galactono-1,4-lactone dehydrogenase (CsGalLDH). Error bars represent standard deviation among three qRT-PCR reaction replicates. Data are means of three replicates ± SD.
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f4: Expression level analyses of genes involved in AsA biosynthesis pathway in tea plant leaves.Genes involved in AsA biosynthesis pathway (A) Phosphoglucose isomerase (CsPGI1), (B) (CsPGI2), (C) phosphomannose isomerase (CsPMI), (D) phosphomannose mutase (CsPMM), (E) GDP-d-mannose pyrophosphorylase (CsGMP), (F) GDP-d-mannose-3′,5′-epimerase (CsGME), (G) GDP-l-galactose phosphorylase (CsGGP), (H) l-galactose-1-P phosphatase (CsGPP), (I) l-galactose dehydrogenase (CsGalDH), (J) l-galactono-1,4-lactone dehydrogenase (CsGalLDH). Error bars represent standard deviation among three qRT-PCR reaction replicates. Data are means of three replicates ± SD.

Mentions: The expression levels of 12 genes involved in AsA biosynthesis were detected in leaves at different developmental stages of three tea plant cultivars by qRT-PCR (Fig. 4). The expression level of CsPGI1 showed an upward trend in both ‘Anjibaicha’ and ‘Yingshuang’ during three developmental stages (Fig. 4A). The expression level of CsPGI2 peaked at stage 2 then declined in ‘Huangjinya’ and ‘Yingshuang’. By contrast, the transcription level of CsPGI2 decreased at the stage 2 and then increased in ‘Anjibaicha’ (Fig. 4B). CsGalLDH displayed a continuous decrease at three developmental stages in both ‘Anjibaicha’ and ‘Yingshuang’ (Fig. 4J). The expression levels of CsGGP and GalLDH experienced a similar upward trend in ‘Huangjinya’ (Fig. 4G,J).


Transcriptomic analysis of the biosynthesis, recycling, and distribution of ascorbic acid during leaf development in tea plant ( Camellia sinensis (L.) O. Kuntze)
Expression level analyses of genes involved in AsA biosynthesis pathway in tea plant leaves.Genes involved in AsA biosynthesis pathway (A) Phosphoglucose isomerase (CsPGI1), (B) (CsPGI2), (C) phosphomannose isomerase (CsPMI), (D) phosphomannose mutase (CsPMM), (E) GDP-d-mannose pyrophosphorylase (CsGMP), (F) GDP-d-mannose-3′,5′-epimerase (CsGME), (G) GDP-l-galactose phosphorylase (CsGGP), (H) l-galactose-1-P phosphatase (CsGPP), (I) l-galactose dehydrogenase (CsGalDH), (J) l-galactono-1,4-lactone dehydrogenase (CsGalLDH). Error bars represent standard deviation among three qRT-PCR reaction replicates. Data are means of three replicates ± SD.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5385563&req=5

f4: Expression level analyses of genes involved in AsA biosynthesis pathway in tea plant leaves.Genes involved in AsA biosynthesis pathway (A) Phosphoglucose isomerase (CsPGI1), (B) (CsPGI2), (C) phosphomannose isomerase (CsPMI), (D) phosphomannose mutase (CsPMM), (E) GDP-d-mannose pyrophosphorylase (CsGMP), (F) GDP-d-mannose-3′,5′-epimerase (CsGME), (G) GDP-l-galactose phosphorylase (CsGGP), (H) l-galactose-1-P phosphatase (CsGPP), (I) l-galactose dehydrogenase (CsGalDH), (J) l-galactono-1,4-lactone dehydrogenase (CsGalLDH). Error bars represent standard deviation among three qRT-PCR reaction replicates. Data are means of three replicates ± SD.
Mentions: The expression levels of 12 genes involved in AsA biosynthesis were detected in leaves at different developmental stages of three tea plant cultivars by qRT-PCR (Fig. 4). The expression level of CsPGI1 showed an upward trend in both ‘Anjibaicha’ and ‘Yingshuang’ during three developmental stages (Fig. 4A). The expression level of CsPGI2 peaked at stage 2 then declined in ‘Huangjinya’ and ‘Yingshuang’. By contrast, the transcription level of CsPGI2 decreased at the stage 2 and then increased in ‘Anjibaicha’ (Fig. 4B). CsGalLDH displayed a continuous decrease at three developmental stages in both ‘Anjibaicha’ and ‘Yingshuang’ (Fig. 4J). The expression levels of CsGGP and GalLDH experienced a similar upward trend in ‘Huangjinya’ (Fig. 4G,J).

View Article: PubMed Central - PubMed

ABSTRACT

Ascorbic acid (AsA), known as vitamin C, is an essential nutrient for humans and mainly absorbed from food. Tea plant (Camellia sinensis (L.) O. Kuntze) leaves can be a dietary source of AsA for humans. However, experimental evidence on the biosynthesis, recycling pathway and distribution of AsA during leaf development in tea plants is unclear. To gain insight into the mechanism and distribution of AsA in the tea plant leaf, we identified 18 related genes involved in AsA biosynthesis and recycling pathway based on the transcriptome database of tea plants. Tea plant leaves were used as samples at different developmental stages. AsA contens in tea plant leaves at three developmental stages were measured by reversed-phase high-performance liquid chromatography (RP-HPLC). The correlations between expression levels of these genes and AsA contents during the development of tea plant leaves were discussed. Results indicated that the l-galactose pathway might be the primary pathway of AsA biosynthesis in tea plant leaves. CsMDHAR and CsGGP might play a regulatory role in AsA accumulation in the leaves of three cultivars of tea plants. These findings may provide a further glimpse to improve the AsA accumulation in tea plants and the commercial quality of tea.

No MeSH data available.