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Transcriptomic analysis of the biosynthesis, recycling, and distribution of ascorbic acid during leaf development in tea plant ( Camellia sinensis (L.) O. Kuntze)

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ABSTRACT

Ascorbic acid (AsA), known as vitamin C, is an essential nutrient for humans and mainly absorbed from food. Tea plant (Camellia sinensis (L.) O. Kuntze) leaves can be a dietary source of AsA for humans. However, experimental evidence on the biosynthesis, recycling pathway and distribution of AsA during leaf development in tea plants is unclear. To gain insight into the mechanism and distribution of AsA in the tea plant leaf, we identified 18 related genes involved in AsA biosynthesis and recycling pathway based on the transcriptome database of tea plants. Tea plant leaves were used as samples at different developmental stages. AsA contens in tea plant leaves at three developmental stages were measured by reversed-phase high-performance liquid chromatography (RP-HPLC). The correlations between expression levels of these genes and AsA contents during the development of tea plant leaves were discussed. Results indicated that the l-galactose pathway might be the primary pathway of AsA biosynthesis in tea plant leaves. CsMDHAR and CsGGP might play a regulatory role in AsA accumulation in the leaves of three cultivars of tea plants. These findings may provide a further glimpse to improve the AsA accumulation in tea plants and the commercial quality of tea.

No MeSH data available.


AsA contents of leaves at three developmental stages in three tea plant cultivars.Error bars represent standard deviation among three independent replicates. Data are means of three replicates ± SD.
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f2: AsA contents of leaves at three developmental stages in three tea plant cultivars.Error bars represent standard deviation among three independent replicates. Data are means of three replicates ± SD.

Mentions: The AsA content was measured at three leaf developmental stages in three tea plant cultivars by RP-HPLC (Figs 2 and 3). The highest concentration of AsA was detected at the first stage in ‘Huangjinya’ (79.81 mg/100 g FW), whereas the lowest content was observed at stage 3 in ‘Yingshuang’ (29.43 mg/100 g FW). The AsA content initially increased and then evidently decreased in ‘Yingshuang’ and ‘Anjibaicha’. A significant reduction of AsA content was observed during leaf development in ‘Huangjinya’.


Transcriptomic analysis of the biosynthesis, recycling, and distribution of ascorbic acid during leaf development in tea plant ( Camellia sinensis (L.) O. Kuntze)
AsA contents of leaves at three developmental stages in three tea plant cultivars.Error bars represent standard deviation among three independent replicates. Data are means of three replicates ± SD.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5385563&req=5

f2: AsA contents of leaves at three developmental stages in three tea plant cultivars.Error bars represent standard deviation among three independent replicates. Data are means of three replicates ± SD.
Mentions: The AsA content was measured at three leaf developmental stages in three tea plant cultivars by RP-HPLC (Figs 2 and 3). The highest concentration of AsA was detected at the first stage in ‘Huangjinya’ (79.81 mg/100 g FW), whereas the lowest content was observed at stage 3 in ‘Yingshuang’ (29.43 mg/100 g FW). The AsA content initially increased and then evidently decreased in ‘Yingshuang’ and ‘Anjibaicha’. A significant reduction of AsA content was observed during leaf development in ‘Huangjinya’.

View Article: PubMed Central - PubMed

ABSTRACT

Ascorbic acid (AsA), known as vitamin C, is an essential nutrient for humans and mainly absorbed from food. Tea plant (Camellia sinensis (L.) O. Kuntze) leaves can be a dietary source of AsA for humans. However, experimental evidence on the biosynthesis, recycling pathway and distribution of AsA during leaf development in tea plants is unclear. To gain insight into the mechanism and distribution of AsA in the tea plant leaf, we identified 18 related genes involved in AsA biosynthesis and recycling pathway based on the transcriptome database of tea plants. Tea plant leaves were used as samples at different developmental stages. AsA contens in tea plant leaves at three developmental stages were measured by reversed-phase high-performance liquid chromatography (RP-HPLC). The correlations between expression levels of these genes and AsA contents during the development of tea plant leaves were discussed. Results indicated that the l-galactose pathway might be the primary pathway of AsA biosynthesis in tea plant leaves. CsMDHAR and CsGGP might play a regulatory role in AsA accumulation in the leaves of three cultivars of tea plants. These findings may provide a further glimpse to improve the AsA accumulation in tea plants and the commercial quality of tea.

No MeSH data available.