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Deterministic bead-in-droplet ejection utilizing an integrated plug-in bead dispenser for single bead – based applications

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ABSTRACT

This paper presents a deterministic bead-in-droplet ejection (BIDE) technique that regulates the precise distribution of microbeads in an ejected droplet. The deterministic BIDE was realized through the effective integration of a microfluidic single-particle handling technique with a liquid dispensing system. The integrated bead dispenser facilitates the transfer of the desired number of beads into a dispensing volume and the on-demand ejection of bead-encapsulated droplets. Single bead–encapsulated droplets were ejected every 3 s without any failure. Multiple-bead dispensing with deterministic control of the number of beads was demonstrated to emphasize the originality and quality of the proposed dispensing technique. The dispenser was mounted using a plug-socket type connection, and the dispensing process was completely automated using a programmed sequence without any microscopic observation. To demonstrate a potential application of the technique, bead-based streptavidin–biotin binding assay in an evaporating droplet was conducted using ultralow numbers of beads. The results evidenced the number of beads in the droplet crucially influences the reliability of the assay. Therefore, the proposed deterministic bead-in-droplet technology can be utilized to deliver desired beads onto a reaction site, particularly to reliably and efficiently enrich and detect target biomolecules.

No MeSH data available.


Design of the plug-in bead dispensing module.(a) Schematic of the dispensing module; (b) top view of the plug-in bead dispenser; (c) schematic of the deterministic BIDE mechanism and an enlarged view of the plug-in bead dispenser indicated in (b) by the black-dashed lines. Red and blue arrows indicate flow direction in normal and dispensing modes, respectively.
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f1: Design of the plug-in bead dispensing module.(a) Schematic of the dispensing module; (b) top view of the plug-in bead dispenser; (c) schematic of the deterministic BIDE mechanism and an enlarged view of the plug-in bead dispenser indicated in (b) by the black-dashed lines. Red and blue arrows indicate flow direction in normal and dispensing modes, respectively.

Mentions: The plug-in bead-dispensing module is composed of a liquid cartridge and a plug-in bead dispenser. The cartridge comprises female sockets, liquid reservoirs, detachable caps, and solenoid valves (Fig. 1a), and the plug-in bead dispenser comprises a pneumatic inlet, a bead inlet and outlet, a dispensing inlet, a dispensing nozzle, and four male plugs assembled onto the back of each inlet and outlet (Fig. 1b). On the basis of our experience in a previous study, the plug–socket connection technique was employed to allow easy and reversible sealing against fluid flow and air compression22. The inlet reservoir, dispensing reservoir, outlet container, and pneumatic line 1 are connected to the bead inlet of the plug-in dispenser, the dispensing inlet, bead outlet, and pneumatic inlet via the plug–socket connection, respectively. Compressed air is supplied to the inlet, outlet, and dispensing reservoirs to induce fluid flow into the microchannel inside the dispenser. Compressed air is also supplied to pneumatic line 1 to operate the pneumatic valve of the dispenser.


Deterministic bead-in-droplet ejection utilizing an integrated plug-in bead dispenser for single bead – based applications
Design of the plug-in bead dispensing module.(a) Schematic of the dispensing module; (b) top view of the plug-in bead dispenser; (c) schematic of the deterministic BIDE mechanism and an enlarged view of the plug-in bead dispenser indicated in (b) by the black-dashed lines. Red and blue arrows indicate flow direction in normal and dispensing modes, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5385560&req=5

f1: Design of the plug-in bead dispensing module.(a) Schematic of the dispensing module; (b) top view of the plug-in bead dispenser; (c) schematic of the deterministic BIDE mechanism and an enlarged view of the plug-in bead dispenser indicated in (b) by the black-dashed lines. Red and blue arrows indicate flow direction in normal and dispensing modes, respectively.
Mentions: The plug-in bead-dispensing module is composed of a liquid cartridge and a plug-in bead dispenser. The cartridge comprises female sockets, liquid reservoirs, detachable caps, and solenoid valves (Fig. 1a), and the plug-in bead dispenser comprises a pneumatic inlet, a bead inlet and outlet, a dispensing inlet, a dispensing nozzle, and four male plugs assembled onto the back of each inlet and outlet (Fig. 1b). On the basis of our experience in a previous study, the plug–socket connection technique was employed to allow easy and reversible sealing against fluid flow and air compression22. The inlet reservoir, dispensing reservoir, outlet container, and pneumatic line 1 are connected to the bead inlet of the plug-in dispenser, the dispensing inlet, bead outlet, and pneumatic inlet via the plug–socket connection, respectively. Compressed air is supplied to the inlet, outlet, and dispensing reservoirs to induce fluid flow into the microchannel inside the dispenser. Compressed air is also supplied to pneumatic line 1 to operate the pneumatic valve of the dispenser.

View Article: PubMed Central - PubMed

ABSTRACT

This paper presents a deterministic bead-in-droplet ejection (BIDE) technique that regulates the precise distribution of microbeads in an ejected droplet. The deterministic BIDE was realized through the effective integration of a microfluidic single-particle handling technique with a liquid dispensing system. The integrated bead dispenser facilitates the transfer of the desired number of beads into a dispensing volume and the on-demand ejection of bead-encapsulated droplets. Single bead–encapsulated droplets were ejected every 3 s without any failure. Multiple-bead dispensing with deterministic control of the number of beads was demonstrated to emphasize the originality and quality of the proposed dispensing technique. The dispenser was mounted using a plug-socket type connection, and the dispensing process was completely automated using a programmed sequence without any microscopic observation. To demonstrate a potential application of the technique, bead-based streptavidin–biotin binding assay in an evaporating droplet was conducted using ultralow numbers of beads. The results evidenced the number of beads in the droplet crucially influences the reliability of the assay. Therefore, the proposed deterministic bead-in-droplet technology can be utilized to deliver desired beads onto a reaction site, particularly to reliably and efficiently enrich and detect target biomolecules.

No MeSH data available.