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Genetic dissection of seed oil and protein content and identification of networks associated with oil content in Brassica napus

View Article: PubMed Central - PubMed

ABSTRACT

High-density linkage maps can improve the precision of QTL localization. A high-density SNP-based linkage map containing 3207 markers covering 3072.7 cM of the Brassica napus genome was constructed in the KenC-8 × N53-2 (KNDH) population. A total of 67 and 38 QTLs for seed oil and protein content were identified with an average confidence interval of 5.26 and 4.38 cM, which could explain up to 22.24% and 27.48% of the phenotypic variation, respectively. Thirty-eight associated genomic regions from BSA overlapped with and/or narrowed the SOC-QTLs, further confirming the QTL mapping results based on the high-density linkage map. Potential candidates related to acyl-lipid and seed storage underlying SOC and SPC, respectively, were identified and analyzed, among which six were checked and showed expression differences between the two parents during different embryonic developmental periods. A large primary carbohydrate pathway based on potential candidates underlying SOC- and SPC-QTLs, and interaction networks based on potential candidates underlying SOC-QTLs, was constructed to dissect the complex mechanism based on metabolic and gene regulatory features, respectively. Accurate QTL mapping and potential candidates identified based on high-density linkage map and BSA analyses provide new insights into the complex genetic mechanism of oil and protein accumulation in the seeds of rapeseed.

No MeSH data available.


qRT-PCR results for six potential candidates.Blue bars represent KenC-8, and orange bars represent N53-2. The X-axis indicates 15, 25, and 35 days after flowering, whereas the Y-axis indicates relative expression levels.
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f4: qRT-PCR results for six potential candidates.Blue bars represent KenC-8, and orange bars represent N53-2. The X-axis indicates 15, 25, and 35 days after flowering, whereas the Y-axis indicates relative expression levels.

Mentions: Six potential candidates (BnaA08g12780D, BnaA09g48250D, BnaC03g65980D, BnaC03g67820D, BnaC05g43390D and BnaC05g44510D) that were reported to be involved in acyl-lipid metabolism in B. napus or A. thaliana were selected to measure the expression levels in the developing seed at 15, 25, and 35 days after flowering from both parents. All six genes showed significant expression difference between parents during different developmental periods (Fig. 4). The relative expression level of BnaA09g48250D, which was homologous to the AtCAC2 gene encoding BC (subunit of ACCase), from the overlapping region of AGR_A9-12 and the major QTL cqOC-A9-10 increased significantly in both parents and were clearly higher in N53-2 during the middle and late embryonic development periods. BnaA08g12780D and BnaC03g67820D, which are homologous to AtFAD6 (oleata desaturase), were determined from cqOC-A8-3 and cqOC-C3-7, their relative expression level was reduced but higher in N53-2 during the late developmental period. BnaC03g65980D encoded FAE1 and was involved in FA elongation, showing a rapid increase in expression throughout the process of embryonic development and a significantly higher level in N53-2 than in KenC-8. Of interest, the relative expression level of BnaC05g44510D located in cqOC-C5-7 and involved in cuticular wax synthesis was clearly higher in N53-2 than in KenC-8 but decreased rapidly and was lower in N53-2 during the late embryonic development period. This phenomenon might be associated with the usage of more precursors for the synthesis of TAG in N53-2, especially during late embryonic development.


Genetic dissection of seed oil and protein content and identification of networks associated with oil content in Brassica napus
qRT-PCR results for six potential candidates.Blue bars represent KenC-8, and orange bars represent N53-2. The X-axis indicates 15, 25, and 35 days after flowering, whereas the Y-axis indicates relative expression levels.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5385559&req=5

f4: qRT-PCR results for six potential candidates.Blue bars represent KenC-8, and orange bars represent N53-2. The X-axis indicates 15, 25, and 35 days after flowering, whereas the Y-axis indicates relative expression levels.
Mentions: Six potential candidates (BnaA08g12780D, BnaA09g48250D, BnaC03g65980D, BnaC03g67820D, BnaC05g43390D and BnaC05g44510D) that were reported to be involved in acyl-lipid metabolism in B. napus or A. thaliana were selected to measure the expression levels in the developing seed at 15, 25, and 35 days after flowering from both parents. All six genes showed significant expression difference between parents during different developmental periods (Fig. 4). The relative expression level of BnaA09g48250D, which was homologous to the AtCAC2 gene encoding BC (subunit of ACCase), from the overlapping region of AGR_A9-12 and the major QTL cqOC-A9-10 increased significantly in both parents and were clearly higher in N53-2 during the middle and late embryonic development periods. BnaA08g12780D and BnaC03g67820D, which are homologous to AtFAD6 (oleata desaturase), were determined from cqOC-A8-3 and cqOC-C3-7, their relative expression level was reduced but higher in N53-2 during the late developmental period. BnaC03g65980D encoded FAE1 and was involved in FA elongation, showing a rapid increase in expression throughout the process of embryonic development and a significantly higher level in N53-2 than in KenC-8. Of interest, the relative expression level of BnaC05g44510D located in cqOC-C5-7 and involved in cuticular wax synthesis was clearly higher in N53-2 than in KenC-8 but decreased rapidly and was lower in N53-2 during the late embryonic development period. This phenomenon might be associated with the usage of more precursors for the synthesis of TAG in N53-2, especially during late embryonic development.

View Article: PubMed Central - PubMed

ABSTRACT

High-density linkage maps can improve the precision of QTL localization. A high-density SNP-based linkage map containing 3207 markers covering 3072.7 cM of the Brassica napus genome was constructed in the KenC-8 × N53-2 (KNDH) population. A total of 67 and 38 QTLs for seed oil and protein content were identified with an average confidence interval of 5.26 and 4.38 cM, which could explain up to 22.24% and 27.48% of the phenotypic variation, respectively. Thirty-eight associated genomic regions from BSA overlapped with and/or narrowed the SOC-QTLs, further confirming the QTL mapping results based on the high-density linkage map. Potential candidates related to acyl-lipid and seed storage underlying SOC and SPC, respectively, were identified and analyzed, among which six were checked and showed expression differences between the two parents during different embryonic developmental periods. A large primary carbohydrate pathway based on potential candidates underlying SOC- and SPC-QTLs, and interaction networks based on potential candidates underlying SOC-QTLs, was constructed to dissect the complex mechanism based on metabolic and gene regulatory features, respectively. Accurate QTL mapping and potential candidates identified based on high-density linkage map and BSA analyses provide new insights into the complex genetic mechanism of oil and protein accumulation in the seeds of rapeseed.

No MeSH data available.