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Involvement of a velvet protein ClVelB in the regulation of vegetative differentiation, oxidative stress response, secondary metabolism, and virulence in Curvularia lunata

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ABSTRACT

The ortholog of Aspergillus nidulans VelB, which is known as ClVelB, was studied to gain a broader insight into the functions of a velvet protein in Curvularia lunata. With the expected common and specific functions of ClVelB, the deletion of clvelB results in similar though not identical phenotypes. The pathogenicity assays revealed that ΔClVelB was impaired in colonizing the host tissue, which corresponds to the finding that ClVelB controls the production of conidia and the methyl 5-(hydroxymethyl) furan-2-carboxylate toxin in C. lunata. However, the deletion of clvelB led to the increase in aerial hyphae and melanin formation. In addition, ΔClVelB showed a decreased sensitivity to iprodione and fludioxonil fungicides and a decreased resistance to cell wall-damaging agents and osmotic stress and tolerance to H2O2. The ultrastructural analysis indicated that the cell wall of ΔClVelB became thinner, which agrees with the finding that the accumulated level of glycerol in ΔClVelB is lower than the wild-type. Furthermore, the interaction of ClVelB with ClVeA and ClVosA was identified in the present research through the yeast two-hybrid and bimolecular fluorescence complementation assays. Results indicate that ClVelB plays a vital role in the regulation of various cellular processes in C. lunata.

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Virulence of the WT (CX-3), clvelB deletion mutant (ΔClVelB), and complemented strain (ClVelB-C) on maize leaves.clvelB deletion mutants are impaired in the colonization of maize leaves. Detached leaves of HUANGZAO-4 were inoculated with conidial suspensions and incubated on two layers of filter papers moisturized with 10 mM 6-Benzyladenine (6-BA) in Petri dishes at 28 °C for 96 h.
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f12: Virulence of the WT (CX-3), clvelB deletion mutant (ΔClVelB), and complemented strain (ClVelB-C) on maize leaves.clvelB deletion mutants are impaired in the colonization of maize leaves. Detached leaves of HUANGZAO-4 were inoculated with conidial suspensions and incubated on two layers of filter papers moisturized with 10 mM 6-Benzyladenine (6-BA) in Petri dishes at 28 °C for 96 h.

Mentions: Mycotoxin M5HF2C has been described as one of most important virulence factors in C. lunata2. We further assayed the infective ability of ΔClVelB on maize leaves because the deletion of clvelB compromised the ability of C. lunata to produce M5HF2C. The penetration and establishment of primary lesions by ΔClVelB were similar to those by WT. However, the infection proceeded differentially. The capability of ΔClVelB to colonize the surrounding host tissue was impaired (Fig. 12). In any case, the lesion sizes on maize leaves inoculated with ΔClVelB decreased significantly compared to those inoculated with WT, indicating that ClVelB was essential to the complete virulence in C. lunata.


Involvement of a velvet protein ClVelB in the regulation of vegetative differentiation, oxidative stress response, secondary metabolism, and virulence in Curvularia lunata
Virulence of the WT (CX-3), clvelB deletion mutant (ΔClVelB), and complemented strain (ClVelB-C) on maize leaves.clvelB deletion mutants are impaired in the colonization of maize leaves. Detached leaves of HUANGZAO-4 were inoculated with conidial suspensions and incubated on two layers of filter papers moisturized with 10 mM 6-Benzyladenine (6-BA) in Petri dishes at 28 °C for 96 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5385503&req=5

f12: Virulence of the WT (CX-3), clvelB deletion mutant (ΔClVelB), and complemented strain (ClVelB-C) on maize leaves.clvelB deletion mutants are impaired in the colonization of maize leaves. Detached leaves of HUANGZAO-4 were inoculated with conidial suspensions and incubated on two layers of filter papers moisturized with 10 mM 6-Benzyladenine (6-BA) in Petri dishes at 28 °C for 96 h.
Mentions: Mycotoxin M5HF2C has been described as one of most important virulence factors in C. lunata2. We further assayed the infective ability of ΔClVelB on maize leaves because the deletion of clvelB compromised the ability of C. lunata to produce M5HF2C. The penetration and establishment of primary lesions by ΔClVelB were similar to those by WT. However, the infection proceeded differentially. The capability of ΔClVelB to colonize the surrounding host tissue was impaired (Fig. 12). In any case, the lesion sizes on maize leaves inoculated with ΔClVelB decreased significantly compared to those inoculated with WT, indicating that ClVelB was essential to the complete virulence in C. lunata.

View Article: PubMed Central - PubMed

ABSTRACT

The ortholog of Aspergillus nidulans VelB, which is known as ClVelB, was studied to gain a broader insight into the functions of a velvet protein in Curvularia lunata. With the expected common and specific functions of ClVelB, the deletion of clvelB results in similar though not identical phenotypes. The pathogenicity assays revealed that ΔClVelB was impaired in colonizing the host tissue, which corresponds to the finding that ClVelB controls the production of conidia and the methyl 5-(hydroxymethyl) furan-2-carboxylate toxin in C. lunata. However, the deletion of clvelB led to the increase in aerial hyphae and melanin formation. In addition, ΔClVelB showed a decreased sensitivity to iprodione and fludioxonil fungicides and a decreased resistance to cell wall-damaging agents and osmotic stress and tolerance to H2O2. The ultrastructural analysis indicated that the cell wall of ΔClVelB became thinner, which agrees with the finding that the accumulated level of glycerol in ΔClVelB is lower than the wild-type. Furthermore, the interaction of ClVelB with ClVeA and ClVosA was identified in the present research through the yeast two-hybrid and bimolecular fluorescence complementation assays. Results indicate that ClVelB plays a vital role in the regulation of various cellular processes in C. lunata.

No MeSH data available.


Related in: MedlinePlus