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Involvement of a velvet protein ClVelB in the regulation of vegetative differentiation, oxidative stress response, secondary metabolism, and virulence in Curvularia lunata

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ABSTRACT

The ortholog of Aspergillus nidulans VelB, which is known as ClVelB, was studied to gain a broader insight into the functions of a velvet protein in Curvularia lunata. With the expected common and specific functions of ClVelB, the deletion of clvelB results in similar though not identical phenotypes. The pathogenicity assays revealed that ΔClVelB was impaired in colonizing the host tissue, which corresponds to the finding that ClVelB controls the production of conidia and the methyl 5-(hydroxymethyl) furan-2-carboxylate toxin in C. lunata. However, the deletion of clvelB led to the increase in aerial hyphae and melanin formation. In addition, ΔClVelB showed a decreased sensitivity to iprodione and fludioxonil fungicides and a decreased resistance to cell wall-damaging agents and osmotic stress and tolerance to H2O2. The ultrastructural analysis indicated that the cell wall of ΔClVelB became thinner, which agrees with the finding that the accumulated level of glycerol in ΔClVelB is lower than the wild-type. Furthermore, the interaction of ClVelB with ClVeA and ClVosA was identified in the present research through the yeast two-hybrid and bimolecular fluorescence complementation assays. Results indicate that ClVelB plays a vital role in the regulation of various cellular processes in C. lunata.

No MeSH data available.


Related in: MedlinePlus

Effects of the clvelB deletion on hyphal hydrophobicity.20 μl of ddH2O or 2.5% bromophenol blue solution was dropped on the colony surfaces of the WT strain (CX-3), clvelB deletion mutant (ΔClVelB), and complemented strain (ClVelB-C), and photographed 10 min later. The droplet did not disperse on the colony of ΔClVelB, WT, and ClVelB-C.
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f10: Effects of the clvelB deletion on hyphal hydrophobicity.20 μl of ddH2O or 2.5% bromophenol blue solution was dropped on the colony surfaces of the WT strain (CX-3), clvelB deletion mutant (ΔClVelB), and complemented strain (ClVelB-C), and photographed 10 min later. The droplet did not disperse on the colony of ΔClVelB, WT, and ClVelB-C.

Mentions: In numerous fungal species, the cell surface of aerial hyphae shows a distinct hydrophobic feature13. The deletion of fgvelB leads to loss of function to maintain the hydrophobicity of the hyphal surface in Fusarium graminearum14. To confirm if clvelB has the same function in C. lunata, 20 μl drops of 2.5% bromophenol blue solution or ddH2O were added to each strain surface. Both the 2.5% bromophenol blue solution and the ddH2O maintained spherical droplets on the surface of the ΔClVelB colony without being absorbed or extended for more than 30 min, thereby demonstrating the strong hydrophobicity of the ΔClVelB hyphae, which is similar to those of WT, and the complemented strain (Fig. 10). These results indicate that ClVelB did not contribute in regulating the hyphal hydrophobicity of C. lunata.


Involvement of a velvet protein ClVelB in the regulation of vegetative differentiation, oxidative stress response, secondary metabolism, and virulence in Curvularia lunata
Effects of the clvelB deletion on hyphal hydrophobicity.20 μl of ddH2O or 2.5% bromophenol blue solution was dropped on the colony surfaces of the WT strain (CX-3), clvelB deletion mutant (ΔClVelB), and complemented strain (ClVelB-C), and photographed 10 min later. The droplet did not disperse on the colony of ΔClVelB, WT, and ClVelB-C.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5385503&req=5

f10: Effects of the clvelB deletion on hyphal hydrophobicity.20 μl of ddH2O or 2.5% bromophenol blue solution was dropped on the colony surfaces of the WT strain (CX-3), clvelB deletion mutant (ΔClVelB), and complemented strain (ClVelB-C), and photographed 10 min later. The droplet did not disperse on the colony of ΔClVelB, WT, and ClVelB-C.
Mentions: In numerous fungal species, the cell surface of aerial hyphae shows a distinct hydrophobic feature13. The deletion of fgvelB leads to loss of function to maintain the hydrophobicity of the hyphal surface in Fusarium graminearum14. To confirm if clvelB has the same function in C. lunata, 20 μl drops of 2.5% bromophenol blue solution or ddH2O were added to each strain surface. Both the 2.5% bromophenol blue solution and the ddH2O maintained spherical droplets on the surface of the ΔClVelB colony without being absorbed or extended for more than 30 min, thereby demonstrating the strong hydrophobicity of the ΔClVelB hyphae, which is similar to those of WT, and the complemented strain (Fig. 10). These results indicate that ClVelB did not contribute in regulating the hyphal hydrophobicity of C. lunata.

View Article: PubMed Central - PubMed

ABSTRACT

The ortholog of Aspergillus nidulans VelB, which is known as ClVelB, was studied to gain a broader insight into the functions of a velvet protein in Curvularia lunata. With the expected common and specific functions of ClVelB, the deletion of clvelB results in similar though not identical phenotypes. The pathogenicity assays revealed that ΔClVelB was impaired in colonizing the host tissue, which corresponds to the finding that ClVelB controls the production of conidia and the methyl 5-(hydroxymethyl) furan-2-carboxylate toxin in C. lunata. However, the deletion of clvelB led to the increase in aerial hyphae and melanin formation. In addition, ΔClVelB showed a decreased sensitivity to iprodione and fludioxonil fungicides and a decreased resistance to cell wall-damaging agents and osmotic stress and tolerance to H2O2. The ultrastructural analysis indicated that the cell wall of ΔClVelB became thinner, which agrees with the finding that the accumulated level of glycerol in ΔClVelB is lower than the wild-type. Furthermore, the interaction of ClVelB with ClVeA and ClVosA was identified in the present research through the yeast two-hybrid and bimolecular fluorescence complementation assays. Results indicate that ClVelB plays a vital role in the regulation of various cellular processes in C. lunata.

No MeSH data available.


Related in: MedlinePlus