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Colorimetric Detection Based on Localised Surface Plasmon Resonance Optical Characteristics for the Detection of Hydrogen Peroxide Using Acacia Gum – Stabilised Silver Nanoparticles

View Article: PubMed Central - PubMed

ABSTRACT

The use of nanoparticles in sensing is attracting the interest of many researchers. The aim of this work was to fabricate Acacia gum–stabilised silver nanoparticles (SNPs) using green chemistry to use them as a highly sensitive and cost-effective localised surface plasmon resonance (LSPR) colorimeter sensor for the determination of reactive oxygen species, such as hydrogen peroxide (H2O2). Silver nanoparticles were fabricated by the reduction of an inorganic precursor silver nitrate solution (AgNO3) using white sugar as the reducing reagent and Acacia gum as the stabilising reagent and a sonication bath to form uniform silver nanoparticles. The fabricated nanoparticles were characterised by visual observation, ultraviolet-visible (UV-Vis) spectrophotometry, transmission electron microscopy (TEM) analysis, energy-dispersive X-ray spectroscopy (EDAX), thermogravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FT-IR). The TEM micrographs of the synthesised nanoparticles showed the presence of spherical nanoparticles with sizes of approximately 10 nm. The EDAX spectrum result confirmed the presence of silver (58%), carbon (30%), and oxygen (12%). Plasmon colorimetric sensing of H2O2 solution was investigated by introducing H2O2 solution into Acacia gum–capped SNP dispersion, and the change in the LSPR band in the UV-Vis region of spectra was monitored. In this study, it was found that the yellow colour of Acacia gum–stabilised SNPs gradually changed to transparent, and moreover, a remarkable change in the LSPR absorbance strength was observed. The calibration curve was linear over 0.1–0.00001 M H2O2, with a correlation estimation (R2) of .953. This was due to the aggregation of SNPs following introduction of the H2O2 solution. Furthermore, the fabricated SNPs were successfully used to detect H2O2 solution in a liquid milk sample, thereby demonstrating the ability of the fabricated SNPs to detect H2O2 solution in liquid milk samples. This work showed that Acacia gum–stabilised SNPs may have the potential as a colour indicator in medical and environmental applications.

No MeSH data available.


(A) Photograph of a test tube containing Acacia gum–stabilised silver nanoparticles (SNPs) with different concentrations of H2O2 solution (0.5-0.00001 M) showing the change in the solution colour of Acacia gum–stabilised SNPs after 60 minutes of reaction time at room temperature. (B) Localised surface plasmon resonance optical characteristics change with time due to the aggregation of SNPs induced by H2O2 solution.
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f8-10.1177_1177390116684686: (A) Photograph of a test tube containing Acacia gum–stabilised silver nanoparticles (SNPs) with different concentrations of H2O2 solution (0.5-0.00001 M) showing the change in the solution colour of Acacia gum–stabilised SNPs after 60 minutes of reaction time at room temperature. (B) Localised surface plasmon resonance optical characteristics change with time due to the aggregation of SNPs induced by H2O2 solution.

Mentions: For quantitative determination of the H2O2 concentration using the Acacia gum–stabilised SNPs, different concentrations of H2O2 solution (0.5-0.00001 M) prepared using 20 mM phosphate buffer solution (pH 7.0) were added to Acacia gum–stabilised SNPs at a volume ratio of 1:1.5, and the UV-Vis absorption spectra were recorded after 60 minutes of adding H2O2 solution to evaluate the calibration characteristics. Figure 8A shows a photograph of the Acacia gum–stabilised SNPs after introducing different concentrations of H2O2 solution showing the change in colour of the Acacia gum–stabilized SNPs from yellow by LSPR absorption to transparent. The change of the solution colour was due to the aggregation of SNPs induced by H2O2 solution.66 A higher concentration of H2O2 solution causes a higher aggregation and a colour change of SNPs that can observed by the naked eye, with the colour change being directly proportional to the amount of H2O2 solution added to Acacia gum–stabilised SNP solution.


Colorimetric Detection Based on Localised Surface Plasmon Resonance Optical Characteristics for the Detection of Hydrogen Peroxide Using Acacia Gum – Stabilised Silver Nanoparticles
(A) Photograph of a test tube containing Acacia gum–stabilised silver nanoparticles (SNPs) with different concentrations of H2O2 solution (0.5-0.00001 M) showing the change in the solution colour of Acacia gum–stabilised SNPs after 60 minutes of reaction time at room temperature. (B) Localised surface plasmon resonance optical characteristics change with time due to the aggregation of SNPs induced by H2O2 solution.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5385488&req=5

f8-10.1177_1177390116684686: (A) Photograph of a test tube containing Acacia gum–stabilised silver nanoparticles (SNPs) with different concentrations of H2O2 solution (0.5-0.00001 M) showing the change in the solution colour of Acacia gum–stabilised SNPs after 60 minutes of reaction time at room temperature. (B) Localised surface plasmon resonance optical characteristics change with time due to the aggregation of SNPs induced by H2O2 solution.
Mentions: For quantitative determination of the H2O2 concentration using the Acacia gum–stabilised SNPs, different concentrations of H2O2 solution (0.5-0.00001 M) prepared using 20 mM phosphate buffer solution (pH 7.0) were added to Acacia gum–stabilised SNPs at a volume ratio of 1:1.5, and the UV-Vis absorption spectra were recorded after 60 minutes of adding H2O2 solution to evaluate the calibration characteristics. Figure 8A shows a photograph of the Acacia gum–stabilised SNPs after introducing different concentrations of H2O2 solution showing the change in colour of the Acacia gum–stabilized SNPs from yellow by LSPR absorption to transparent. The change of the solution colour was due to the aggregation of SNPs induced by H2O2 solution.66 A higher concentration of H2O2 solution causes a higher aggregation and a colour change of SNPs that can observed by the naked eye, with the colour change being directly proportional to the amount of H2O2 solution added to Acacia gum–stabilised SNP solution.

View Article: PubMed Central - PubMed

ABSTRACT

The use of nanoparticles in sensing is attracting the interest of many researchers. The aim of this work was to fabricate Acacia gum–stabilised silver nanoparticles (SNPs) using green chemistry to use them as a highly sensitive and cost-effective localised surface plasmon resonance (LSPR) colorimeter sensor for the determination of reactive oxygen species, such as hydrogen peroxide (H2O2). Silver nanoparticles were fabricated by the reduction of an inorganic precursor silver nitrate solution (AgNO3) using white sugar as the reducing reagent and Acacia gum as the stabilising reagent and a sonication bath to form uniform silver nanoparticles. The fabricated nanoparticles were characterised by visual observation, ultraviolet-visible (UV-Vis) spectrophotometry, transmission electron microscopy (TEM) analysis, energy-dispersive X-ray spectroscopy (EDAX), thermogravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FT-IR). The TEM micrographs of the synthesised nanoparticles showed the presence of spherical nanoparticles with sizes of approximately 10 nm. The EDAX spectrum result confirmed the presence of silver (58%), carbon (30%), and oxygen (12%). Plasmon colorimetric sensing of H2O2 solution was investigated by introducing H2O2 solution into Acacia gum–capped SNP dispersion, and the change in the LSPR band in the UV-Vis region of spectra was monitored. In this study, it was found that the yellow colour of Acacia gum–stabilised SNPs gradually changed to transparent, and moreover, a remarkable change in the LSPR absorbance strength was observed. The calibration curve was linear over 0.1–0.00001 M H2O2, with a correlation estimation (R2) of .953. This was due to the aggregation of SNPs following introduction of the H2O2 solution. Furthermore, the fabricated SNPs were successfully used to detect H2O2 solution in a liquid milk sample, thereby demonstrating the ability of the fabricated SNPs to detect H2O2 solution in liquid milk samples. This work showed that Acacia gum–stabilised SNPs may have the potential as a colour indicator in medical and environmental applications.

No MeSH data available.