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Colorimetric Detection Based on Localised Surface Plasmon Resonance Optical Characteristics for the Detection of Hydrogen Peroxide Using Acacia Gum – Stabilised Silver Nanoparticles

View Article: PubMed Central - PubMed

ABSTRACT

The use of nanoparticles in sensing is attracting the interest of many researchers. The aim of this work was to fabricate Acacia gum–stabilised silver nanoparticles (SNPs) using green chemistry to use them as a highly sensitive and cost-effective localised surface plasmon resonance (LSPR) colorimeter sensor for the determination of reactive oxygen species, such as hydrogen peroxide (H2O2). Silver nanoparticles were fabricated by the reduction of an inorganic precursor silver nitrate solution (AgNO3) using white sugar as the reducing reagent and Acacia gum as the stabilising reagent and a sonication bath to form uniform silver nanoparticles. The fabricated nanoparticles were characterised by visual observation, ultraviolet-visible (UV-Vis) spectrophotometry, transmission electron microscopy (TEM) analysis, energy-dispersive X-ray spectroscopy (EDAX), thermogravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FT-IR). The TEM micrographs of the synthesised nanoparticles showed the presence of spherical nanoparticles with sizes of approximately 10 nm. The EDAX spectrum result confirmed the presence of silver (58%), carbon (30%), and oxygen (12%). Plasmon colorimetric sensing of H2O2 solution was investigated by introducing H2O2 solution into Acacia gum–capped SNP dispersion, and the change in the LSPR band in the UV-Vis region of spectra was monitored. In this study, it was found that the yellow colour of Acacia gum–stabilised SNPs gradually changed to transparent, and moreover, a remarkable change in the LSPR absorbance strength was observed. The calibration curve was linear over 0.1–0.00001 M H2O2, with a correlation estimation (R2) of .953. This was due to the aggregation of SNPs following introduction of the H2O2 solution. Furthermore, the fabricated SNPs were successfully used to detect H2O2 solution in a liquid milk sample, thereby demonstrating the ability of the fabricated SNPs to detect H2O2 solution in liquid milk samples. This work showed that Acacia gum–stabilised SNPs may have the potential as a colour indicator in medical and environmental applications.

No MeSH data available.


Related in: MedlinePlus

Transmission electron microscopy micrographs of Acacia gum–stabilised silver nanoparticles after introducing 0.001 M hydrogen peroxide solution.
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f11-10.1177_1177390116684686: Transmission electron microscopy micrographs of Acacia gum–stabilised silver nanoparticles after introducing 0.001 M hydrogen peroxide solution.

Mentions: The fabricated Acacia gum–stabilised SNPs were examined by TEM analysis after adding H2O2 solution. As can be seen in Figure 11, it was observed that the morphology of the Acacia gum–stabilised SNPs was completely changed after introducing H2O2 solution and the spherical colloids could not been seen, compared with Figure 2. Before adding H2O2 solution, the Acacia gum–stabilised SNPs were stable and dispersed in the dispersion medium, resulting in effective excitation of the LSPR band absorbance. On the contrary, when H2O2 solution was added to the Acacia gum–stabilised SNPs, the nanoparticles were aggregated by the catalytic reaction between SNPs and H2O2 solution, and the aggregation of nanoparticles causes the LSPR band absorbance strength to decrease.63,64


Colorimetric Detection Based on Localised Surface Plasmon Resonance Optical Characteristics for the Detection of Hydrogen Peroxide Using Acacia Gum – Stabilised Silver Nanoparticles
Transmission electron microscopy micrographs of Acacia gum–stabilised silver nanoparticles after introducing 0.001 M hydrogen peroxide solution.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5385488&req=5

f11-10.1177_1177390116684686: Transmission electron microscopy micrographs of Acacia gum–stabilised silver nanoparticles after introducing 0.001 M hydrogen peroxide solution.
Mentions: The fabricated Acacia gum–stabilised SNPs were examined by TEM analysis after adding H2O2 solution. As can be seen in Figure 11, it was observed that the morphology of the Acacia gum–stabilised SNPs was completely changed after introducing H2O2 solution and the spherical colloids could not been seen, compared with Figure 2. Before adding H2O2 solution, the Acacia gum–stabilised SNPs were stable and dispersed in the dispersion medium, resulting in effective excitation of the LSPR band absorbance. On the contrary, when H2O2 solution was added to the Acacia gum–stabilised SNPs, the nanoparticles were aggregated by the catalytic reaction between SNPs and H2O2 solution, and the aggregation of nanoparticles causes the LSPR band absorbance strength to decrease.63,64

View Article: PubMed Central - PubMed

ABSTRACT

The use of nanoparticles in sensing is attracting the interest of many researchers. The aim of this work was to fabricate Acacia gum–stabilised silver nanoparticles (SNPs) using green chemistry to use them as a highly sensitive and cost-effective localised surface plasmon resonance (LSPR) colorimeter sensor for the determination of reactive oxygen species, such as hydrogen peroxide (H2O2). Silver nanoparticles were fabricated by the reduction of an inorganic precursor silver nitrate solution (AgNO3) using white sugar as the reducing reagent and Acacia gum as the stabilising reagent and a sonication bath to form uniform silver nanoparticles. The fabricated nanoparticles were characterised by visual observation, ultraviolet-visible (UV-Vis) spectrophotometry, transmission electron microscopy (TEM) analysis, energy-dispersive X-ray spectroscopy (EDAX), thermogravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FT-IR). The TEM micrographs of the synthesised nanoparticles showed the presence of spherical nanoparticles with sizes of approximately 10 nm. The EDAX spectrum result confirmed the presence of silver (58%), carbon (30%), and oxygen (12%). Plasmon colorimetric sensing of H2O2 solution was investigated by introducing H2O2 solution into Acacia gum–capped SNP dispersion, and the change in the LSPR band in the UV-Vis region of spectra was monitored. In this study, it was found that the yellow colour of Acacia gum–stabilised SNPs gradually changed to transparent, and moreover, a remarkable change in the LSPR absorbance strength was observed. The calibration curve was linear over 0.1–0.00001 M H2O2, with a correlation estimation (R2) of .953. This was due to the aggregation of SNPs following introduction of the H2O2 solution. Furthermore, the fabricated SNPs were successfully used to detect H2O2 solution in a liquid milk sample, thereby demonstrating the ability of the fabricated SNPs to detect H2O2 solution in liquid milk samples. This work showed that Acacia gum–stabilised SNPs may have the potential as a colour indicator in medical and environmental applications.

No MeSH data available.


Related in: MedlinePlus