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Lentivirus-mediated knockdown of P27RF-Rho inhibits hepatocellular carcinoma cell growth

View Article: PubMed Central - PubMed

ABSTRACT

Aim of the study: To investigate the effects of P27RF-Rho on hepatocellular carcinoma (HCC) cell growth and explore the possibility of using it as a novel therapeutic target for liver cancer treatment.

Material and methods: P27RF-Rho in HCC cells was silenced by lentivirus-mediated RNA interference, and the silencing effect was verified by RT-PCR. Cell proliferation was determined by MTT and clone formation assay. Cell cycle phase and apoptosis were detected through FACS. The expression level of cell growth, apoptosis, and metastasis associated genes was detected by quantitative PCR.

Results: Lentivirus-mediated P27RF-Rho knockdown inhibited HCC cell growth and clone formation. P27RF-Rho silence induced cell cycle arrest and apoptosis. The mRNA level of genes associated with cell cycle, apoptosis, and invasion also significantly altered after P27RF-Rho knockdown. Cyclin A, CDK2, BCL-2, and MMP-9 were down-regulated. P27 and Bax were up-regulated.

Conclusions: P27RF-Rho knockdown inhibits HCC cell growth, and P27RF-Rho is probably a promising target for HCC treatment.

No MeSH data available.


Related in: MedlinePlus

Cell cycle and apoptosis in different groups determined by FACS. A) More cells arrested in G0/G1 phase and less cells stayed in G2/M phase in P27RF-Rho knockdown group. B) P27RF-Rho knockdown led to more cell apoptosis
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f0002: Cell cycle and apoptosis in different groups determined by FACS. A) More cells arrested in G0/G1 phase and less cells stayed in G2/M phase in P27RF-Rho knockdown group. B) P27RF-Rho knockdown led to more cell apoptosis

Mentions: In the P27RF-Rho-siRNA group, cells in the G0/G1 phase were significantly more than in the parental Bel7402 and negative control groups (70.70 ±20.23% vs. 59.43 ±1.80% and 61.81 ±2.67%, p < 0.01). Cells in the G2/M phase in the P27RF-Rho-siRNA group were noticeably less than in the parental Bel7402 and negative control groups (2.78 ±0.98% vs. 12.59 ±0.74% and 12.58 ±1.02%, p < 0.01), suggesting that cell growth was inhibited after P27RF-Rho knockdown. More apoptosis occurred in the P27RF-Rho knockdown group than in the parental Bel7402 and negative control groups (45.86 ±2.71% vs. 8.55 ±0.90% and 7.87 ±1.21%, p<0.01). No significant difference was found in S phase among the three groups (Fig. 2).


Lentivirus-mediated knockdown of P27RF-Rho inhibits hepatocellular carcinoma cell growth
Cell cycle and apoptosis in different groups determined by FACS. A) More cells arrested in G0/G1 phase and less cells stayed in G2/M phase in P27RF-Rho knockdown group. B) P27RF-Rho knockdown led to more cell apoptosis
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5385476&req=5

f0002: Cell cycle and apoptosis in different groups determined by FACS. A) More cells arrested in G0/G1 phase and less cells stayed in G2/M phase in P27RF-Rho knockdown group. B) P27RF-Rho knockdown led to more cell apoptosis
Mentions: In the P27RF-Rho-siRNA group, cells in the G0/G1 phase were significantly more than in the parental Bel7402 and negative control groups (70.70 ±20.23% vs. 59.43 ±1.80% and 61.81 ±2.67%, p < 0.01). Cells in the G2/M phase in the P27RF-Rho-siRNA group were noticeably less than in the parental Bel7402 and negative control groups (2.78 ±0.98% vs. 12.59 ±0.74% and 12.58 ±1.02%, p < 0.01), suggesting that cell growth was inhibited after P27RF-Rho knockdown. More apoptosis occurred in the P27RF-Rho knockdown group than in the parental Bel7402 and negative control groups (45.86 ±2.71% vs. 8.55 ±0.90% and 7.87 ±1.21%, p<0.01). No significant difference was found in S phase among the three groups (Fig. 2).

View Article: PubMed Central - PubMed

ABSTRACT

Aim of the study: To investigate the effects of P27RF-Rho on hepatocellular carcinoma (HCC) cell growth and explore the possibility of using it as a novel therapeutic target for liver cancer treatment.

Material and methods: P27RF-Rho in HCC cells was silenced by lentivirus-mediated RNA interference, and the silencing effect was verified by RT-PCR. Cell proliferation was determined by MTT and clone formation assay. Cell cycle phase and apoptosis were detected through FACS. The expression level of cell growth, apoptosis, and metastasis associated genes was detected by quantitative PCR.

Results: Lentivirus-mediated P27RF-Rho knockdown inhibited HCC cell growth and clone formation. P27RF-Rho silence induced cell cycle arrest and apoptosis. The mRNA level of genes associated with cell cycle, apoptosis, and invasion also significantly altered after P27RF-Rho knockdown. Cyclin A, CDK2, BCL-2, and MMP-9 were down-regulated. P27 and Bax were up-regulated.

Conclusions: P27RF-Rho knockdown inhibits HCC cell growth, and P27RF-Rho is probably a promising target for HCC treatment.

No MeSH data available.


Related in: MedlinePlus