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Effects of Acupuncture on mRNA Levels of Apoptotic Factors in Perihematomal Brain Tissue During the Acute Phase of Cerebral Hemorrhage

View Article: PubMed Central - PubMed

ABSTRACT

Background: To explore the time-dependent effects of acupuncture on mRNA levels of the apoptotic factors BCL-2 and BAX in a rat cerebral hemorrhage model, slow injection of autologous blood to the caudate nucleus was used to generate the cerebral hemorrhage model.

Material/methods: A sham surgery control group, groups with acupuncture applied 3, 9, 24, and 48 hours after model induction, and time-matched model-only control groups were used. In situ hybridization was used to detect BCL-2 and BAX mRNA expression, and semi-quantitative RT-PCR was used to measure the expression.

Results: The number of BCL-2 and BAX mRNA-positive cells significantly increased during the acute phase of cerebral hemorrhage. BCL-2 mRNA was significantly upregulated in acupuncture groups compared to other groups, whereas BAX mRNA levels in the acupuncture groups were lower in the other groups, except for the sham surgery group. Additionally, earlier acupuncture intervention was associated with a lower ratio of expression between the two genes. Changes in BCL-2 and BAX mRNA expression were consistent with changes in the number of cells positive for BCL-2 and BAX mRNA; however, the change in the expression ratio was consistent with the change in the number of cells positive for BCL-2 mRNA, but opposite to the change in the number of cells positive for BAX mRNA.

Conclusions: Acupuncture ameliorated changes in expression of apoptotic factors in the brain induced by acute cerebral hemorrhage and may thus protect the brain, with greater efficacy when the delay before acupuncture was minimized.

No MeSH data available.


Comparisons of BCL-2/BAX mRNA ratios between groups. Between groups: compared with the sham surgery group * p<0.05, compared with the same time point model group $p<0.05; within the group: compared with the 48 hour group #p<0.05, compared with the 24 hour group @p<0.05, compared with the 9 hour group &p<0.05.
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f7-medscimonit-23-1522: Comparisons of BCL-2/BAX mRNA ratios between groups. Between groups: compared with the sham surgery group * p<0.05, compared with the same time point model group $p<0.05; within the group: compared with the 48 hour group #p<0.05, compared with the 24 hour group @p<0.05, compared with the 9 hour group &p<0.05.

Mentions: As shown in Table 3 and Figure 7, the ratio of the BCL-2 mRNA level to the BAX mRNA level was significantly higher in the sham surgery and acupuncture groups than in the model groups at the same time point (p<0.05), with the highest ratio in the acupuncture groups. The ratio of BCL-2 mRNA level to BAX mRNA level was significantly higher in the 3 hour and 9 hour model groups compared to the 24 hour and 48 hour model groups (p<0.05), and no significant differences were found between the 3 hour and 9 hour model groups or between the 24 hour and 48 hour model groups. The ratio of BCL-2 mRNA level to BAX mRNA level was also significantly higher in the 3 hour and 9 hour acupuncture groups compared to the 24 hour and 48 hour acupuncture groups (p<0.05), and significantly higher in the 24 hour acupuncture group than in the 48 hour group (p<0.05).


Effects of Acupuncture on mRNA Levels of Apoptotic Factors in Perihematomal Brain Tissue During the Acute Phase of Cerebral Hemorrhage
Comparisons of BCL-2/BAX mRNA ratios between groups. Between groups: compared with the sham surgery group * p<0.05, compared with the same time point model group $p<0.05; within the group: compared with the 48 hour group #p<0.05, compared with the 24 hour group @p<0.05, compared with the 9 hour group &p<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC5384619&req=5

f7-medscimonit-23-1522: Comparisons of BCL-2/BAX mRNA ratios between groups. Between groups: compared with the sham surgery group * p<0.05, compared with the same time point model group $p<0.05; within the group: compared with the 48 hour group #p<0.05, compared with the 24 hour group @p<0.05, compared with the 9 hour group &p<0.05.
Mentions: As shown in Table 3 and Figure 7, the ratio of the BCL-2 mRNA level to the BAX mRNA level was significantly higher in the sham surgery and acupuncture groups than in the model groups at the same time point (p<0.05), with the highest ratio in the acupuncture groups. The ratio of BCL-2 mRNA level to BAX mRNA level was significantly higher in the 3 hour and 9 hour model groups compared to the 24 hour and 48 hour model groups (p<0.05), and no significant differences were found between the 3 hour and 9 hour model groups or between the 24 hour and 48 hour model groups. The ratio of BCL-2 mRNA level to BAX mRNA level was also significantly higher in the 3 hour and 9 hour acupuncture groups compared to the 24 hour and 48 hour acupuncture groups (p<0.05), and significantly higher in the 24 hour acupuncture group than in the 48 hour group (p<0.05).

View Article: PubMed Central - PubMed

ABSTRACT

Background: To explore the time-dependent effects of acupuncture on mRNA levels of the apoptotic factors BCL-2 and BAX in a rat cerebral hemorrhage model, slow injection of autologous blood to the caudate nucleus was used to generate the cerebral hemorrhage model.

Material/methods: A sham surgery control group, groups with acupuncture applied 3, 9, 24, and 48 hours after model induction, and time-matched model-only control groups were used. In situ hybridization was used to detect BCL-2 and BAX mRNA expression, and semi-quantitative RT-PCR was used to measure the expression.

Results: The number of BCL-2 and BAX mRNA-positive cells significantly increased during the acute phase of cerebral hemorrhage. BCL-2 mRNA was significantly upregulated in acupuncture groups compared to other groups, whereas BAX mRNA levels in the acupuncture groups were lower in the other groups, except for the sham surgery group. Additionally, earlier acupuncture intervention was associated with a lower ratio of expression between the two genes. Changes in BCL-2 and BAX mRNA expression were consistent with changes in the number of cells positive for BCL-2 and BAX mRNA; however, the change in the expression ratio was consistent with the change in the number of cells positive for BCL-2 mRNA, but opposite to the change in the number of cells positive for BAX mRNA.

Conclusions: Acupuncture ameliorated changes in expression of apoptotic factors in the brain induced by acute cerebral hemorrhage and may thus protect the brain, with greater efficacy when the delay before acupuncture was minimized.

No MeSH data available.