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Effects of Acupuncture on mRNA Levels of Apoptotic Factors in Perihematomal Brain Tissue During the Acute Phase of Cerebral Hemorrhage

View Article: PubMed Central - PubMed

ABSTRACT

Background: To explore the time-dependent effects of acupuncture on mRNA levels of the apoptotic factors BCL-2 and BAX in a rat cerebral hemorrhage model, slow injection of autologous blood to the caudate nucleus was used to generate the cerebral hemorrhage model.

Material/methods: A sham surgery control group, groups with acupuncture applied 3, 9, 24, and 48 hours after model induction, and time-matched model-only control groups were used. In situ hybridization was used to detect BCL-2 and BAX mRNA expression, and semi-quantitative RT-PCR was used to measure the expression.

Results: The number of BCL-2 and BAX mRNA-positive cells significantly increased during the acute phase of cerebral hemorrhage. BCL-2 mRNA was significantly upregulated in acupuncture groups compared to other groups, whereas BAX mRNA levels in the acupuncture groups were lower in the other groups, except for the sham surgery group. Additionally, earlier acupuncture intervention was associated with a lower ratio of expression between the two genes. Changes in BCL-2 and BAX mRNA expression were consistent with changes in the number of cells positive for BCL-2 and BAX mRNA; however, the change in the expression ratio was consistent with the change in the number of cells positive for BCL-2 mRNA, but opposite to the change in the number of cells positive for BAX mRNA.

Conclusions: Acupuncture ameliorated changes in expression of apoptotic factors in the brain induced by acute cerebral hemorrhage and may thus protect the brain, with greater efficacy when the delay before acupuncture was minimized.

No MeSH data available.


(A–I) Sham surgery group; 3, 9, 24, and 48 hour model groups; 3, 9, 24, and 48 hour acupuncture groups, respectively.
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f4-medscimonit-23-1522: (A–I) Sham surgery group; 3, 9, 24, and 48 hour model groups; 3, 9, 24, and 48 hour acupuncture groups, respectively.

Mentions: As shown in Figure 4, BAX mRNA was expressed at different levels in the cytoplasm in perihematomal brain tissues after acute cerebral hemorrhage, and the cytoplasm was stained brown or tan. Cells positive for BAX mRNA had varied shapes and sizes and were unevenly and sporadically distributed among normal cells. Positive cells were rarely seen in the sham surgery group. The rate of positive cells significantly increased in all model subgroups but was similar among the 3, 9, 24, and 48 hour model groups. The number of positive cells in the acupuncture subgroups was significantly higher than in the sham surgery group, but significantly lower than in the model subgroups at the same time point, with the 3 hour and 9 hour acupuncture groups exhibiting the fewest positive cells, followed by the 24 hour and 48 hour acupuncture groups.


Effects of Acupuncture on mRNA Levels of Apoptotic Factors in Perihematomal Brain Tissue During the Acute Phase of Cerebral Hemorrhage
(A–I) Sham surgery group; 3, 9, 24, and 48 hour model groups; 3, 9, 24, and 48 hour acupuncture groups, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC5384619&req=5

f4-medscimonit-23-1522: (A–I) Sham surgery group; 3, 9, 24, and 48 hour model groups; 3, 9, 24, and 48 hour acupuncture groups, respectively.
Mentions: As shown in Figure 4, BAX mRNA was expressed at different levels in the cytoplasm in perihematomal brain tissues after acute cerebral hemorrhage, and the cytoplasm was stained brown or tan. Cells positive for BAX mRNA had varied shapes and sizes and were unevenly and sporadically distributed among normal cells. Positive cells were rarely seen in the sham surgery group. The rate of positive cells significantly increased in all model subgroups but was similar among the 3, 9, 24, and 48 hour model groups. The number of positive cells in the acupuncture subgroups was significantly higher than in the sham surgery group, but significantly lower than in the model subgroups at the same time point, with the 3 hour and 9 hour acupuncture groups exhibiting the fewest positive cells, followed by the 24 hour and 48 hour acupuncture groups.

View Article: PubMed Central - PubMed

ABSTRACT

Background: To explore the time-dependent effects of acupuncture on mRNA levels of the apoptotic factors BCL-2 and BAX in a rat cerebral hemorrhage model, slow injection of autologous blood to the caudate nucleus was used to generate the cerebral hemorrhage model.

Material/methods: A sham surgery control group, groups with acupuncture applied 3, 9, 24, and 48 hours after model induction, and time-matched model-only control groups were used. In situ hybridization was used to detect BCL-2 and BAX mRNA expression, and semi-quantitative RT-PCR was used to measure the expression.

Results: The number of BCL-2 and BAX mRNA-positive cells significantly increased during the acute phase of cerebral hemorrhage. BCL-2 mRNA was significantly upregulated in acupuncture groups compared to other groups, whereas BAX mRNA levels in the acupuncture groups were lower in the other groups, except for the sham surgery group. Additionally, earlier acupuncture intervention was associated with a lower ratio of expression between the two genes. Changes in BCL-2 and BAX mRNA expression were consistent with changes in the number of cells positive for BCL-2 and BAX mRNA; however, the change in the expression ratio was consistent with the change in the number of cells positive for BCL-2 mRNA, but opposite to the change in the number of cells positive for BAX mRNA.

Conclusions: Acupuncture ameliorated changes in expression of apoptotic factors in the brain induced by acute cerebral hemorrhage and may thus protect the brain, with greater efficacy when the delay before acupuncture was minimized.

No MeSH data available.