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Effects of Acupuncture on mRNA Levels of Apoptotic Factors in Perihematomal Brain Tissue During the Acute Phase of Cerebral Hemorrhage

View Article: PubMed Central - PubMed

ABSTRACT

Background: To explore the time-dependent effects of acupuncture on mRNA levels of the apoptotic factors BCL-2 and BAX in a rat cerebral hemorrhage model, slow injection of autologous blood to the caudate nucleus was used to generate the cerebral hemorrhage model.

Material/methods: A sham surgery control group, groups with acupuncture applied 3, 9, 24, and 48 hours after model induction, and time-matched model-only control groups were used. In situ hybridization was used to detect BCL-2 and BAX mRNA expression, and semi-quantitative RT-PCR was used to measure the expression.

Results: The number of BCL-2 and BAX mRNA-positive cells significantly increased during the acute phase of cerebral hemorrhage. BCL-2 mRNA was significantly upregulated in acupuncture groups compared to other groups, whereas BAX mRNA levels in the acupuncture groups were lower in the other groups, except for the sham surgery group. Additionally, earlier acupuncture intervention was associated with a lower ratio of expression between the two genes. Changes in BCL-2 and BAX mRNA expression were consistent with changes in the number of cells positive for BCL-2 and BAX mRNA; however, the change in the expression ratio was consistent with the change in the number of cells positive for BCL-2 mRNA, but opposite to the change in the number of cells positive for BAX mRNA.

Conclusions: Acupuncture ameliorated changes in expression of apoptotic factors in the brain induced by acute cerebral hemorrhage and may thus protect the brain, with greater efficacy when the delay before acupuncture was minimized.

No MeSH data available.


In situ hybridization images showing BCL-2 expression, 400×. (A) Sham operation group, (B) 3 hour model group, (C) 9 hour model group, (D) 24 hour model group, (E) 48 hour model group, (F) 3 hour acupuncture group, (G) 9 hour acupuncture group, (H) 24 hour acupuncture group, (I) 48 hour acupuncture group.
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f2-medscimonit-23-1522: In situ hybridization images showing BCL-2 expression, 400×. (A) Sham operation group, (B) 3 hour model group, (C) 9 hour model group, (D) 24 hour model group, (E) 48 hour model group, (F) 3 hour acupuncture group, (G) 9 hour acupuncture group, (H) 24 hour acupuncture group, (I) 48 hour acupuncture group.

Mentions: BCL-2 mRNA was expressed at different levels in the cytoplasm in perihematomal brain tissues of rats with acute cerebral hemorrhage, and cytoplasm positive for BCL-2 mRNA exhibited a brown or tan color (Figure 2). Cells positive for BCL-2 mRNA were unevenly and sporadically distributed among normal cells, with varied shapes and sizes. In the sham surgery group, positive cells were rarely seen, whereas in the acupuncture subgroups, the number of positive cells increased significantly, especially in the 3 hour and 24 hour acupuncture groups. In subgroups of the model group, the number of positive cells increased compared with the sham surgery group, but significantly decreased compared with the acupuncture groups at the corresponding time points (with the exception of the 9 hour model group, in which the rate of positive cells was similar to that in the 9 hour acupuncture group). Among the subgroups of the model group, the 3 hour model group showed the fewest positive cells.


Effects of Acupuncture on mRNA Levels of Apoptotic Factors in Perihematomal Brain Tissue During the Acute Phase of Cerebral Hemorrhage
In situ hybridization images showing BCL-2 expression, 400×. (A) Sham operation group, (B) 3 hour model group, (C) 9 hour model group, (D) 24 hour model group, (E) 48 hour model group, (F) 3 hour acupuncture group, (G) 9 hour acupuncture group, (H) 24 hour acupuncture group, (I) 48 hour acupuncture group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5384619&req=5

f2-medscimonit-23-1522: In situ hybridization images showing BCL-2 expression, 400×. (A) Sham operation group, (B) 3 hour model group, (C) 9 hour model group, (D) 24 hour model group, (E) 48 hour model group, (F) 3 hour acupuncture group, (G) 9 hour acupuncture group, (H) 24 hour acupuncture group, (I) 48 hour acupuncture group.
Mentions: BCL-2 mRNA was expressed at different levels in the cytoplasm in perihematomal brain tissues of rats with acute cerebral hemorrhage, and cytoplasm positive for BCL-2 mRNA exhibited a brown or tan color (Figure 2). Cells positive for BCL-2 mRNA were unevenly and sporadically distributed among normal cells, with varied shapes and sizes. In the sham surgery group, positive cells were rarely seen, whereas in the acupuncture subgroups, the number of positive cells increased significantly, especially in the 3 hour and 24 hour acupuncture groups. In subgroups of the model group, the number of positive cells increased compared with the sham surgery group, but significantly decreased compared with the acupuncture groups at the corresponding time points (with the exception of the 9 hour model group, in which the rate of positive cells was similar to that in the 9 hour acupuncture group). Among the subgroups of the model group, the 3 hour model group showed the fewest positive cells.

View Article: PubMed Central - PubMed

ABSTRACT

Background: To explore the time-dependent effects of acupuncture on mRNA levels of the apoptotic factors BCL-2 and BAX in a rat cerebral hemorrhage model, slow injection of autologous blood to the caudate nucleus was used to generate the cerebral hemorrhage model.

Material/methods: A sham surgery control group, groups with acupuncture applied 3, 9, 24, and 48 hours after model induction, and time-matched model-only control groups were used. In situ hybridization was used to detect BCL-2 and BAX mRNA expression, and semi-quantitative RT-PCR was used to measure the expression.

Results: The number of BCL-2 and BAX mRNA-positive cells significantly increased during the acute phase of cerebral hemorrhage. BCL-2 mRNA was significantly upregulated in acupuncture groups compared to other groups, whereas BAX mRNA levels in the acupuncture groups were lower in the other groups, except for the sham surgery group. Additionally, earlier acupuncture intervention was associated with a lower ratio of expression between the two genes. Changes in BCL-2 and BAX mRNA expression were consistent with changes in the number of cells positive for BCL-2 and BAX mRNA; however, the change in the expression ratio was consistent with the change in the number of cells positive for BCL-2 mRNA, but opposite to the change in the number of cells positive for BAX mRNA.

Conclusions: Acupuncture ameliorated changes in expression of apoptotic factors in the brain induced by acute cerebral hemorrhage and may thus protect the brain, with greater efficacy when the delay before acupuncture was minimized.

No MeSH data available.