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Intraperitoneal Injection Is Not a Suitable Administration Route for Single-Walled Carbon Nanotubes in Biomedical Applications

View Article: PubMed Central - PubMed

ABSTRACT

Given the extensive application of carbon nanotubes (CNTs) in biomedical fields, there is increasing concern regarding unintentional health impacts. Research into safe usage is therefore increasingly necessary. This study investigated the responses of the mouse brain to single-walled CNTs (SWCNTs) delivered via intraperitoneal (IP) injection and compared these results with the previous study where SWCNTs were delivered via intravenous (IV) injection so as to explore which administration route is potentially better for SWCNTs application. This study suggests SWCNTs delivered via IP injection can have negative effects on the mouse brain through oxidative stress and inflammation at high concentration exposure, but these responses were not consistent and showed no dose-dependent effect. In a previous study, the results showed that IV-delivered SWCNTs induced a more consistent and dose-dependent effect. The comparison of the 2 studies suggested that using SWCNTs at a safe dosage delivered via IV injection may be a better administration route for SWCNTs in biomedical applications.

No MeSH data available.


Related in: MedlinePlus

Histological alterations (×40) seen in mice brains after SWCNTs exposure (H&E staining). Each of the 6 images (1-6) correspond to 1 of the 6 different exposure groups: 0, 3. 125, 6.25, and 12.5 mg/kg/d SWCNTs; 6.25 mg/kg/d SWCNTs + 100 mg/kg/d ascorbic acid (block group); and 100 mg/kg/d ascorbic acid, respectively. H&E indicates hematoxylin and eosin; SWCNTs, single-walled carbon nanotube.
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fig3-1559325816681320: Histological alterations (×40) seen in mice brains after SWCNTs exposure (H&E staining). Each of the 6 images (1-6) correspond to 1 of the 6 different exposure groups: 0, 3. 125, 6.25, and 12.5 mg/kg/d SWCNTs; 6.25 mg/kg/d SWCNTs + 100 mg/kg/d ascorbic acid (block group); and 100 mg/kg/d ascorbic acid, respectively. H&E indicates hematoxylin and eosin; SWCNTs, single-walled carbon nanotube.

Mentions: From the histopathological examination of the brain, histological changes were observed in the pyramidal cells of the CA1 region of the hippocampus. The cells in the hippocampus of the control group were arranged neatly together, with clear edges and polygonal shapes, and the radial apical dendrites stretched to the inner hippocampus. As the exposure concentrations increased, some histopathological lesions appeared. Cells became loosely arranged, disorderly, swelling deformations were observed in the shape of some cells, and the radial apical dendrites shortened or even disappeared (Figure 3).


Intraperitoneal Injection Is Not a Suitable Administration Route for Single-Walled Carbon Nanotubes in Biomedical Applications
Histological alterations (×40) seen in mice brains after SWCNTs exposure (H&E staining). Each of the 6 images (1-6) correspond to 1 of the 6 different exposure groups: 0, 3. 125, 6.25, and 12.5 mg/kg/d SWCNTs; 6.25 mg/kg/d SWCNTs + 100 mg/kg/d ascorbic acid (block group); and 100 mg/kg/d ascorbic acid, respectively. H&E indicates hematoxylin and eosin; SWCNTs, single-walled carbon nanotube.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC5384492&req=5

fig3-1559325816681320: Histological alterations (×40) seen in mice brains after SWCNTs exposure (H&E staining). Each of the 6 images (1-6) correspond to 1 of the 6 different exposure groups: 0, 3. 125, 6.25, and 12.5 mg/kg/d SWCNTs; 6.25 mg/kg/d SWCNTs + 100 mg/kg/d ascorbic acid (block group); and 100 mg/kg/d ascorbic acid, respectively. H&E indicates hematoxylin and eosin; SWCNTs, single-walled carbon nanotube.
Mentions: From the histopathological examination of the brain, histological changes were observed in the pyramidal cells of the CA1 region of the hippocampus. The cells in the hippocampus of the control group were arranged neatly together, with clear edges and polygonal shapes, and the radial apical dendrites stretched to the inner hippocampus. As the exposure concentrations increased, some histopathological lesions appeared. Cells became loosely arranged, disorderly, swelling deformations were observed in the shape of some cells, and the radial apical dendrites shortened or even disappeared (Figure 3).

View Article: PubMed Central - PubMed

ABSTRACT

Given the extensive application of carbon nanotubes (CNTs) in biomedical fields, there is increasing concern regarding unintentional health impacts. Research into safe usage is therefore increasingly necessary. This study investigated the responses of the mouse brain to single-walled CNTs (SWCNTs) delivered via intraperitoneal (IP) injection and compared these results with the previous study where SWCNTs were delivered via intravenous (IV) injection so as to explore which administration route is potentially better for SWCNTs application. This study suggests SWCNTs delivered via IP injection can have negative effects on the mouse brain through oxidative stress and inflammation at high concentration exposure, but these responses were not consistent and showed no dose-dependent effect. In a previous study, the results showed that IV-delivered SWCNTs induced a more consistent and dose-dependent effect. The comparison of the 2 studies suggested that using SWCNTs at a safe dosage delivered via IV injection may be a better administration route for SWCNTs in biomedical applications.

No MeSH data available.


Related in: MedlinePlus