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Mosquito-borne viruses circulating in Kinshasa, Democratic Republic of the Congo

View Article: PubMed Central - PubMed

ABSTRACT

Adult mosquitoes collected in Kinshasa, the Democratic Republic of the Congo (DRC), were screened for viruses in this study.

Approximately 40% of mosquitoes were found to be infected with Alphavirus, Flavivirus, and/or Bunyaviridae.

Chikungunya, o’nyong’nyong, and Rift valley fever viruses were found in mosquitoes.

Habitats favouring mosquito breeding and viral transmission were found in Kinshasa.

Screening for mosquito-borne viruses in humans and livestock in DRC is recommended.

Screening for mosquito-borne viruses in humans and livestock in DRC is recommended.

No MeSH data available.


Related in: MedlinePlus

Detection of chikungunya and o’nyong’nyong viruses in female mosquito pools collected in Kinshasa. Amplification of chikungunya and o’nyong’nyong viruses using reverse transcription PCR (RT-PCR) targeting the 5′ non-translated region. The expected PCR product size for chikungunya was 98 base pairs, while it was 80 base pairs for o’nyong’nyong virus. NC: negative control; LD: ladder. Lane numbers indicate the pool identity.
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fig0010: Detection of chikungunya and o’nyong’nyong viruses in female mosquito pools collected in Kinshasa. Amplification of chikungunya and o’nyong’nyong viruses using reverse transcription PCR (RT-PCR) targeting the 5′ non-translated region. The expected PCR product size for chikungunya was 98 base pairs, while it was 80 base pairs for o’nyong’nyong virus. NC: negative control; LD: ladder. Lane numbers indicate the pool identity.

Mentions: Arboviruses were detected in 12 of the 29 pools (41.4%) and included Alphavirus (eight pools), Flavivirus (nine pools), and Bunyaviridae (five pools) (Table 2). Some pools showed mixed infection with two or three arboviruses. Amplification with specific primers for different members of each genus showed the presence of chikungunya virus, o’nyong’nyong virus (Figure 2), and Rift Valley fever virus in Aedes pools and o’nyong’nyong virus in the Anopheles pool. Positive pools were detected in all of the locations tested within Kinshasa (Table 1).


Mosquito-borne viruses circulating in Kinshasa, Democratic Republic of the Congo
Detection of chikungunya and o’nyong’nyong viruses in female mosquito pools collected in Kinshasa. Amplification of chikungunya and o’nyong’nyong viruses using reverse transcription PCR (RT-PCR) targeting the 5′ non-translated region. The expected PCR product size for chikungunya was 98 base pairs, while it was 80 base pairs for o’nyong’nyong virus. NC: negative control; LD: ladder. Lane numbers indicate the pool identity.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384432&req=5

fig0010: Detection of chikungunya and o’nyong’nyong viruses in female mosquito pools collected in Kinshasa. Amplification of chikungunya and o’nyong’nyong viruses using reverse transcription PCR (RT-PCR) targeting the 5′ non-translated region. The expected PCR product size for chikungunya was 98 base pairs, while it was 80 base pairs for o’nyong’nyong virus. NC: negative control; LD: ladder. Lane numbers indicate the pool identity.
Mentions: Arboviruses were detected in 12 of the 29 pools (41.4%) and included Alphavirus (eight pools), Flavivirus (nine pools), and Bunyaviridae (five pools) (Table 2). Some pools showed mixed infection with two or three arboviruses. Amplification with specific primers for different members of each genus showed the presence of chikungunya virus, o’nyong’nyong virus (Figure 2), and Rift Valley fever virus in Aedes pools and o’nyong’nyong virus in the Anopheles pool. Positive pools were detected in all of the locations tested within Kinshasa (Table 1).

View Article: PubMed Central - PubMed

ABSTRACT

Adult mosquitoes collected in Kinshasa, the Democratic Republic of the Congo (DRC), were screened for viruses in this study.

Approximately 40% of mosquitoes were found to be infected with Alphavirus, Flavivirus, and/or Bunyaviridae.

Chikungunya, o’nyong’nyong, and Rift valley fever viruses were found in mosquitoes.

Habitats favouring mosquito breeding and viral transmission were found in Kinshasa.

Screening for mosquito-borne viruses in humans and livestock in DRC is recommended.

Screening for mosquito-borne viruses in humans and livestock in DRC is recommended.

No MeSH data available.


Related in: MedlinePlus