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miRNA signatures can predict acute liver failure in hepatitis E infected pregnant females

View Article: PubMed Central - PubMed

ABSTRACT

Background: Acute viral hepatitis E (AVH-E) can often result in acute liver failure (ALF) during pregnancy. microRNAs serve as mediators in drug induced liver failure. We investigated their role as a biomarker in predicting ALF due to HEV (ALF-E).

Methods: We performed next generation sequencing and subsequent validation studies in PBMCs of pregnant (P) self limiting AVH-E, ALF due to HEV (ALF-E) and compared with AVH-E in non-pregnant (NP) females and healthy controls.

Findings: Eleven microRNAs were significantly expressed in response to HEV infection; importantly, miR- 431, 654, 1468 and 4435, were distinctly expressed in pregnant self-limiting AVH-E and healthy females (p = 0.0005), but not in ALF-E. Sixteen exclusive microRNAs differentiated ALF-E from self limiting AVH-E in pregnant females. miR-450b which affects cellular proliferation and metabolic processes through RNF20 and SECB was predominanlty upregulated and correlated with poor outcome (ROC 0.958, p = 0.001).

Interpretation: Our results reveal that a specific microRNA profile can predict fatality in ALF-E in pregnancy. These microRNAs could be exploited as prognostic biomarkers and help in the development of new therapeutic interventions.

No MeSH data available.


miR-450b interaction with immune cells showing involvement of various pathways and genes.
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fig0045: miR-450b interaction with immune cells showing involvement of various pathways and genes.

Mentions: miR450b particularly involved with WASF2, PPMIF, SP110, IMP4, SERBP1, CREB3L2, LDLR and many genes which influence the cells including CD8, granulocyte, monocyte, dendritic cells, eosinophils and regulate the Interferon, MYD88, cell proliferation, cell death pathways (Fig. 9).


miRNA signatures can predict acute liver failure in hepatitis E infected pregnant females
miR-450b interaction with immune cells showing involvement of various pathways and genes.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384411&req=5

fig0045: miR-450b interaction with immune cells showing involvement of various pathways and genes.
Mentions: miR450b particularly involved with WASF2, PPMIF, SP110, IMP4, SERBP1, CREB3L2, LDLR and many genes which influence the cells including CD8, granulocyte, monocyte, dendritic cells, eosinophils and regulate the Interferon, MYD88, cell proliferation, cell death pathways (Fig. 9).

View Article: PubMed Central - PubMed

ABSTRACT

Background: Acute viral hepatitis E (AVH-E) can often result in acute liver failure (ALF) during pregnancy. microRNAs serve as mediators in drug induced liver failure. We investigated their role as a biomarker in predicting ALF due to HEV (ALF-E).

Methods: We performed next generation sequencing and subsequent validation studies in PBMCs of pregnant (P) self limiting AVH-E, ALF due to HEV (ALF-E) and compared with AVH-E in non-pregnant (NP) females and healthy controls.

Findings: Eleven microRNAs were significantly expressed in response to HEV infection; importantly, miR- 431, 654, 1468 and 4435, were distinctly expressed in pregnant self-limiting AVH-E and healthy females (p = 0.0005), but not in ALF-E. Sixteen exclusive microRNAs differentiated ALF-E from self limiting AVH-E in pregnant females. miR-450b which affects cellular proliferation and metabolic processes through RNF20 and SECB was predominanlty upregulated and correlated with poor outcome (ROC 0.958, p = 0.001).

Interpretation: Our results reveal that a specific microRNA profile can predict fatality in ALF-E in pregnancy. These microRNAs could be exploited as prognostic biomarkers and help in the development of new therapeutic interventions.

No MeSH data available.