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miRNA signatures can predict acute liver failure in hepatitis E infected pregnant females

View Article: PubMed Central - PubMed

ABSTRACT

Background: Acute viral hepatitis E (AVH-E) can often result in acute liver failure (ALF) during pregnancy. microRNAs serve as mediators in drug induced liver failure. We investigated their role as a biomarker in predicting ALF due to HEV (ALF-E).

Methods: We performed next generation sequencing and subsequent validation studies in PBMCs of pregnant (P) self limiting AVH-E, ALF due to HEV (ALF-E) and compared with AVH-E in non-pregnant (NP) females and healthy controls.

Findings: Eleven microRNAs were significantly expressed in response to HEV infection; importantly, miR- 431, 654, 1468 and 4435, were distinctly expressed in pregnant self-limiting AVH-E and healthy females (p = 0.0005), but not in ALF-E. Sixteen exclusive microRNAs differentiated ALF-E from self limiting AVH-E in pregnant females. miR-450b which affects cellular proliferation and metabolic processes through RNF20 and SECB was predominanlty upregulated and correlated with poor outcome (ROC 0.958, p = 0.001).

Interpretation: Our results reveal that a specific microRNA profile can predict fatality in ALF-E in pregnancy. These microRNAs could be exploited as prognostic biomarkers and help in the development of new therapeutic interventions.

No MeSH data available.


Related in: MedlinePlus

(A) Distinct miR expression in pregnant HEV infection compared to HC. (B) Difference in fold change (log2 ratio) expression of various miRs in three groups. (C) No difference in miR profiling in AVH-E(P) and AVH-E(NP) (D) Fold change expression of miR-590 and miR-624 of various subjects. Data are expressed as box plots in which the horizontal lines indicate the 25th, 50th, and 75th percentiles of the fold change measured by qRT-PCR.
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fig0015: (A) Distinct miR expression in pregnant HEV infection compared to HC. (B) Difference in fold change (log2 ratio) expression of various miRs in three groups. (C) No difference in miR profiling in AVH-E(P) and AVH-E(NP) (D) Fold change expression of miR-590 and miR-624 of various subjects. Data are expressed as box plots in which the horizontal lines indicate the 25th, 50th, and 75th percentiles of the fold change measured by qRT-PCR.

Mentions: To determine the impact of HEV infection on miR expression profile, unsupervised hierarchical clustering analysis was done in pregnant hepatitis patients and compared with controls. It revealed 11 distinct miRs; viz. miRs-561, 590, 624, 627, 651, 877, 3143, 3605, 3656, 3940, and 5189 with <2 fold expression (p < 0.003). as indicators of HEV Infection (Fig. 3A-B). The miR profile in AVH-E(P) and AVH-E(NP) (Fig. 3C) was comparable.


miRNA signatures can predict acute liver failure in hepatitis E infected pregnant females
(A) Distinct miR expression in pregnant HEV infection compared to HC. (B) Difference in fold change (log2 ratio) expression of various miRs in three groups. (C) No difference in miR profiling in AVH-E(P) and AVH-E(NP) (D) Fold change expression of miR-590 and miR-624 of various subjects. Data are expressed as box plots in which the horizontal lines indicate the 25th, 50th, and 75th percentiles of the fold change measured by qRT-PCR.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384411&req=5

fig0015: (A) Distinct miR expression in pregnant HEV infection compared to HC. (B) Difference in fold change (log2 ratio) expression of various miRs in three groups. (C) No difference in miR profiling in AVH-E(P) and AVH-E(NP) (D) Fold change expression of miR-590 and miR-624 of various subjects. Data are expressed as box plots in which the horizontal lines indicate the 25th, 50th, and 75th percentiles of the fold change measured by qRT-PCR.
Mentions: To determine the impact of HEV infection on miR expression profile, unsupervised hierarchical clustering analysis was done in pregnant hepatitis patients and compared with controls. It revealed 11 distinct miRs; viz. miRs-561, 590, 624, 627, 651, 877, 3143, 3605, 3656, 3940, and 5189 with <2 fold expression (p < 0.003). as indicators of HEV Infection (Fig. 3A-B). The miR profile in AVH-E(P) and AVH-E(NP) (Fig. 3C) was comparable.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Acute viral hepatitis E (AVH-E) can often result in acute liver failure (ALF) during pregnancy. microRNAs serve as mediators in drug induced liver failure. We investigated their role as a biomarker in predicting ALF due to HEV (ALF-E).

Methods: We performed next generation sequencing and subsequent validation studies in PBMCs of pregnant (P) self limiting AVH-E, ALF due to HEV (ALF-E) and compared with AVH-E in non-pregnant (NP) females and healthy controls.

Findings: Eleven microRNAs were significantly expressed in response to HEV infection; importantly, miR- 431, 654, 1468 and 4435, were distinctly expressed in pregnant self-limiting AVH-E and healthy females (p = 0.0005), but not in ALF-E. Sixteen exclusive microRNAs differentiated ALF-E from self limiting AVH-E in pregnant females. miR-450b which affects cellular proliferation and metabolic processes through RNF20 and SECB was predominanlty upregulated and correlated with poor outcome (ROC 0.958, p = 0.001).

Interpretation: Our results reveal that a specific microRNA profile can predict fatality in ALF-E in pregnancy. These microRNAs could be exploited as prognostic biomarkers and help in the development of new therapeutic interventions.

No MeSH data available.


Related in: MedlinePlus