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Tert-buthylhydroquinone pre-conditioning exerts dual effects in old female rats exposed to 3-nitropropionic acid

View Article: PubMed Central - PubMed

ABSTRACT

The brain is a very susceptible organ to structural and functional alterations caused by oxidative stress and its vulnerability increases with age. Understanding the antioxidant response activated by the transcription factor Nrf2 has become very important in the aging field in order to activate cellular protection. However, the role of Nrf2 inducers during old age has not been completely understood. Our aim was to activate the Nrf2 pathway by pre-treating old rats with a widely used Nrf2-inducer, tert-buthylhydroquinone (tBHQ), prior to 3-nitropropionic acid (3-NP) insult, in order to evaluate its effects at a behavioral, morphological and biochemical levels. 3-NP has been used to reproduce the biochemical and pathophysiological characteristics of Huntington's disease due to an oxidative effect. Our results suggest that tBHQ confers an important protective effect against 3-NP toxicity; nevertheless, Nrf2 seems not to be the main protective pathway associated to neuroprotection. Hormetic responses include the activation of more than one transcription factor. Nrf2 and NFκB are known to simultaneously initiate different cellular responses against stress by triggering parallel mechanisms, therefore NFκB nuclear accumulation was also evaluated.

No MeSH data available.


Determination of the transcription factor NFkB in the brain of adult and old rats treated with 3-NP after tBHQ PreT. NFκB subunits p65 and p50 in brain tissue from adult (A) and old (B) animals after the different treatments. The Kruskal–Wallis variance analysis followed by Dunn multiple comparison test were done to compare the data using the statistical program NCSS version PASS 15, with a significance: ap<0.05, bp<0.01 vs Ct. The results were obtained from four independent experiments and the data are expressed as mean±SE (Three animals in each group).
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f0040: Determination of the transcription factor NFkB in the brain of adult and old rats treated with 3-NP after tBHQ PreT. NFκB subunits p65 and p50 in brain tissue from adult (A) and old (B) animals after the different treatments. The Kruskal–Wallis variance analysis followed by Dunn multiple comparison test were done to compare the data using the statistical program NCSS version PASS 15, with a significance: ap<0.05, bp<0.01 vs Ct. The results were obtained from four independent experiments and the data are expressed as mean±SE (Three animals in each group).

Mentions: NFκB content in the nucleus during the different treatments in adult and old rats was determined (Fig. 8). Surprisingly, our results indicated that old tBHQ PreT animals had an increased amount of p65 and p50 subunits in the nucleus compared to the controls (over 50%, p<0.05) (Fig. 8B). Paradoxically, in the group of adult animals under the same treatment, p50 decreased (p<0.051) with the PreT and p65 increased (p<0.05) only with tBHQ (Fig. 8A).


Tert-buthylhydroquinone pre-conditioning exerts dual effects in old female rats exposed to 3-nitropropionic acid
Determination of the transcription factor NFkB in the brain of adult and old rats treated with 3-NP after tBHQ PreT. NFκB subunits p65 and p50 in brain tissue from adult (A) and old (B) animals after the different treatments. The Kruskal–Wallis variance analysis followed by Dunn multiple comparison test were done to compare the data using the statistical program NCSS version PASS 15, with a significance: ap<0.05, bp<0.01 vs Ct. The results were obtained from four independent experiments and the data are expressed as mean±SE (Three animals in each group).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384325&req=5

f0040: Determination of the transcription factor NFkB in the brain of adult and old rats treated with 3-NP after tBHQ PreT. NFκB subunits p65 and p50 in brain tissue from adult (A) and old (B) animals after the different treatments. The Kruskal–Wallis variance analysis followed by Dunn multiple comparison test were done to compare the data using the statistical program NCSS version PASS 15, with a significance: ap<0.05, bp<0.01 vs Ct. The results were obtained from four independent experiments and the data are expressed as mean±SE (Three animals in each group).
Mentions: NFκB content in the nucleus during the different treatments in adult and old rats was determined (Fig. 8). Surprisingly, our results indicated that old tBHQ PreT animals had an increased amount of p65 and p50 subunits in the nucleus compared to the controls (over 50%, p<0.05) (Fig. 8B). Paradoxically, in the group of adult animals under the same treatment, p50 decreased (p<0.051) with the PreT and p65 increased (p<0.05) only with tBHQ (Fig. 8A).

View Article: PubMed Central - PubMed

ABSTRACT

The brain is a very susceptible organ to structural and functional alterations caused by oxidative stress and its vulnerability increases with age. Understanding the antioxidant response activated by the transcription factor Nrf2 has become very important in the aging field in order to activate cellular protection. However, the role of Nrf2 inducers during old age has not been completely understood. Our aim was to activate the Nrf2 pathway by pre-treating old rats with a widely used Nrf2-inducer, tert-buthylhydroquinone (tBHQ), prior to 3-nitropropionic acid (3-NP) insult, in order to evaluate its effects at a behavioral, morphological and biochemical levels. 3-NP has been used to reproduce the biochemical and pathophysiological characteristics of Huntington's disease due to an oxidative effect. Our results suggest that tBHQ confers an important protective effect against 3-NP toxicity; nevertheless, Nrf2 seems not to be the main protective pathway associated to neuroprotection. Hormetic responses include the activation of more than one transcription factor. Nrf2 and NF&kappa;B are known to simultaneously initiate different cellular responses against stress by triggering parallel mechanisms, therefore NF&kappa;B nuclear accumulation was also evaluated.

No MeSH data available.