Limits...
Serum pharmacokinetics and coagulation aberration induced by sodium dehydroacetate in male and female Wistar rats

View Article: PubMed Central - PubMed

ABSTRACT

Sodium dehydroacetate (Na-DHA) is used as a preservative in food, animal feeds and cosmetics. Severe haemorrhage in organs and prolongation of coagulation factors in Sprague–Dawley rats has been reported upon oral administration of Na-DHA. We investigated alterations in coagulation parameters and serum pharmacokinetics upon Na-DHA administration. Wistar rats were administered Na-DHA (50–200 mg/kg, p.o.). Weight gain, food consumption, prothrombin time (PT), activated partial thromboplastin time (APTT), serum levels of Vitamin k (Vk)1, and serum levels of Na-DHA were measured, and histopathology undertaken. Significant reductions in body weight, food consumption and serum levels of Vk1, as well as prolonged PT and APTT, were observed. Females were significantly different from males in terms of serum Na-DHA concentration. Congestion in hepatic sinusoids, renal tubules and spleen, as well as haemorrhage in lung alveoli, gastric mucosa, intestinal mucosa and cardiac muscle cells, were observed by histopathological analyses. Correlation of serum Na-DHA via PT and APTT, as well as serum Vk1 via PT and APTT, in females was better than that in males. Female rats are more sensitive than males to Na-DHA. Hence, Na-DHA can induce coagulation aberration in Wistar rats, with higher sensitivity seen in females than males.

No MeSH data available.


Serum concentration of Na-DHA in rats by HPLC.Serum concentrations of Na-DHA were Analyzed by method after Wistar rats were administered Na-DHA (50–200 mg/kg). (A) Male; (B) female.  50 mg/kg;  100 mg/kg;  150 mg/kg;  200 mg/kg.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5384240&req=5

f3: Serum concentration of Na-DHA in rats by HPLC.Serum concentrations of Na-DHA were Analyzed by method after Wistar rats were administered Na-DHA (50–200 mg/kg). (A) Male; (B) female. 50 mg/kg; 100 mg/kg; 150 mg/kg; 200 mg/kg.

Mentions: Data for serum levels of Na-DHA are shown in Fig. 3. At 7–13 days, the Na-DHA concentration (in mg/L) in males was 26–36 in the 200 mg/kg group and 20–25 in the 150 mg/kg group whereas, in females, the respective values were 33–48 and 31–40. There were significant differences in serum levels of Na-DHA between males and females administered 100–200 mg/kg except for the 50 mg/kg group (p = 0.1769), with p = 0.0141 in the 200 mg/kg group and p < 0.001 in the 150 and 100 mg/kg groups (ANOVA).


Serum pharmacokinetics and coagulation aberration induced by sodium dehydroacetate in male and female Wistar rats
Serum concentration of Na-DHA in rats by HPLC.Serum concentrations of Na-DHA were Analyzed by method after Wistar rats were administered Na-DHA (50–200 mg/kg). (A) Male; (B) female.  50 mg/kg;  100 mg/kg;  150 mg/kg;  200 mg/kg.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384240&req=5

f3: Serum concentration of Na-DHA in rats by HPLC.Serum concentrations of Na-DHA were Analyzed by method after Wistar rats were administered Na-DHA (50–200 mg/kg). (A) Male; (B) female. 50 mg/kg; 100 mg/kg; 150 mg/kg; 200 mg/kg.
Mentions: Data for serum levels of Na-DHA are shown in Fig. 3. At 7–13 days, the Na-DHA concentration (in mg/L) in males was 26–36 in the 200 mg/kg group and 20–25 in the 150 mg/kg group whereas, in females, the respective values were 33–48 and 31–40. There were significant differences in serum levels of Na-DHA between males and females administered 100–200 mg/kg except for the 50 mg/kg group (p = 0.1769), with p = 0.0141 in the 200 mg/kg group and p < 0.001 in the 150 and 100 mg/kg groups (ANOVA).

View Article: PubMed Central - PubMed

ABSTRACT

Sodium dehydroacetate (Na-DHA) is used as a preservative in food, animal feeds and cosmetics. Severe haemorrhage in organs and prolongation of coagulation factors in Sprague&ndash;Dawley rats has been reported upon oral administration of Na-DHA. We investigated alterations in coagulation parameters and serum pharmacokinetics upon Na-DHA administration. Wistar rats were administered Na-DHA (50&ndash;200&thinsp;mg/kg, p.o.). Weight gain, food consumption, prothrombin time (PT), activated partial thromboplastin time (APTT), serum levels of Vitamin k (Vk)1, and serum levels of Na-DHA were measured, and histopathology undertaken. Significant reductions in body weight, food consumption and serum levels of Vk1, as well as prolonged PT and APTT, were observed. Females were significantly different from males in terms of serum Na-DHA concentration. Congestion in hepatic sinusoids, renal tubules and spleen, as well as haemorrhage in lung alveoli, gastric mucosa, intestinal mucosa and cardiac muscle cells, were observed by histopathological analyses. Correlation of serum Na-DHA via PT and APTT, as well as serum Vk1 via PT and APTT, in females was better than that in males. Female rats are more sensitive than males to Na-DHA. Hence, Na-DHA can induce coagulation aberration in Wistar rats, with higher sensitivity seen in females than males.

No MeSH data available.