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Streptomyces pactum assisted phytoremediation in Zn/Pb smelter contaminated soil of Feng County and its impact on enzymatic activities

View Article: PubMed Central - PubMed

ABSTRACT

Anthropogenic activities, such as industrial expansion, smelting, mining and agricultural practices, have intensified the discharge of potentially toxic trace elements (PTEs) into the environment, threatening human health and other organisms. To assist phytoremediation by sorghum in soil contaminated by smelters/mines in Feng County (FC), a pot experiment was performed to examine the phytoremediation potential of Streptomyces pactum (Act12) + biochar. The results showed that root uptake of Zn and Cd was reduced by 45 and 22%, respectively, while the uptake of Pb and Cu increased by 17 and 47%, respectively. The shoot and root dry weight and chlorophyll content improved after Act12 inoculation. β-glucosidase, alkaline phosphatase and urease activities in soil improved and antioxidant activities (POD, PAL, PPO) decreased after application of Act12 + biochar due to a reduction in stress from PTEs. BCF, TF and MEA confirmed the role of Act12 in the amelioration and translocation of PTEs. PCA analysis showed a correlation between different factors that affect the translocation of PTEs. Overall, Act12 promoted the phytoremediation of PTEs. Field experiments on Act12 + biochar may provide new insights into the rehabilitation and restoration of soils contaminated by mines.

No MeSH data available.


Phylogenetic tree of Streptomyces pactum (Act12) based on 16S rDNA.
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f8: Phylogenetic tree of Streptomyces pactum (Act12) based on 16S rDNA.

Mentions: Soil contaminated by smelters/mines was collected from Feng County (106°24′54″-107°7′30″ N, 33°34′57″-34°18′21″ E) in Shaanxi Province, China. Samples were collected from contaminated surface soil (0–20 cm), stored in polyethylene bags and transferred to the laboratory. Soil samples were homogenized, air-dried, crushed manually and passed through a 2-mm sieve. Biochar was purchased from Shaanxi Yixin Energy Company, Yangling, China. Pig manure and saw dust were mixed in a 2:1 ratio (dry weight basis) in a PVC composter (130 L) to prepare the PMC according to Li et al.10. Actinomycete (Act12) was isolated from the Qinghai-Tibet Plateau of China24, hereafter identified as Streptomyces pactum, according to its biochemical tests and 16S rDNA sequence analysis (Fig. 8). An Act12 carrier was supplied by Laboratory of Microbial Resources at College of Natural Resources and Environment, Northwest A&F University (Yangling, China). Living spore densities of preparations were 2.6 × 1011 spores g−1.


Streptomyces pactum assisted phytoremediation in Zn/Pb smelter contaminated soil of Feng County and its impact on enzymatic activities
Phylogenetic tree of Streptomyces pactum (Act12) based on 16S rDNA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384225&req=5

f8: Phylogenetic tree of Streptomyces pactum (Act12) based on 16S rDNA.
Mentions: Soil contaminated by smelters/mines was collected from Feng County (106°24′54″-107°7′30″ N, 33°34′57″-34°18′21″ E) in Shaanxi Province, China. Samples were collected from contaminated surface soil (0–20 cm), stored in polyethylene bags and transferred to the laboratory. Soil samples were homogenized, air-dried, crushed manually and passed through a 2-mm sieve. Biochar was purchased from Shaanxi Yixin Energy Company, Yangling, China. Pig manure and saw dust were mixed in a 2:1 ratio (dry weight basis) in a PVC composter (130 L) to prepare the PMC according to Li et al.10. Actinomycete (Act12) was isolated from the Qinghai-Tibet Plateau of China24, hereafter identified as Streptomyces pactum, according to its biochemical tests and 16S rDNA sequence analysis (Fig. 8). An Act12 carrier was supplied by Laboratory of Microbial Resources at College of Natural Resources and Environment, Northwest A&F University (Yangling, China). Living spore densities of preparations were 2.6 × 1011 spores g−1.

View Article: PubMed Central - PubMed

ABSTRACT

Anthropogenic activities, such as industrial expansion, smelting, mining and agricultural practices, have intensified the discharge of potentially toxic trace elements (PTEs) into the environment, threatening human health and other organisms. To assist phytoremediation by sorghum in soil contaminated by smelters/mines in Feng County (FC), a pot experiment was performed to examine the phytoremediation potential of Streptomyces pactum (Act12) + biochar. The results showed that root uptake of Zn and Cd was reduced by 45 and 22%, respectively, while the uptake of Pb and Cu increased by 17 and 47%, respectively. The shoot and root dry weight and chlorophyll content improved after Act12 inoculation. β-glucosidase, alkaline phosphatase and urease activities in soil improved and antioxidant activities (POD, PAL, PPO) decreased after application of Act12 + biochar due to a reduction in stress from PTEs. BCF, TF and MEA confirmed the role of Act12 in the amelioration and translocation of PTEs. PCA analysis showed a correlation between different factors that affect the translocation of PTEs. Overall, Act12 promoted the phytoremediation of PTEs. Field experiments on Act12 + biochar may provide new insights into the rehabilitation and restoration of soils contaminated by mines.

No MeSH data available.