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Streptomyces pactum assisted phytoremediation in Zn/Pb smelter contaminated soil of Feng County and its impact on enzymatic activities

View Article: PubMed Central - PubMed

ABSTRACT

Anthropogenic activities, such as industrial expansion, smelting, mining and agricultural practices, have intensified the discharge of potentially toxic trace elements (PTEs) into the environment, threatening human health and other organisms. To assist phytoremediation by sorghum in soil contaminated by smelters/mines in Feng County (FC), a pot experiment was performed to examine the phytoremediation potential of Streptomyces pactum (Act12) + biochar. The results showed that root uptake of Zn and Cd was reduced by 45 and 22%, respectively, while the uptake of Pb and Cu increased by 17 and 47%, respectively. The shoot and root dry weight and chlorophyll content improved after Act12 inoculation. β-glucosidase, alkaline phosphatase and urease activities in soil improved and antioxidant activities (POD, PAL, PPO) decreased after application of Act12 + biochar due to a reduction in stress from PTEs. BCF, TF and MEA confirmed the role of Act12 in the amelioration and translocation of PTEs. PCA analysis showed a correlation between different factors that affect the translocation of PTEs. Overall, Act12 promoted the phytoremediation of PTEs. Field experiments on Act12 + biochar may provide new insights into the rehabilitation and restoration of soils contaminated by mines.

No MeSH data available.


Effect of soil treatments on enzymatic activities in smelter/mines polluted soil.Data represent the mean of three replicates and error bars are standard deviations.
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f5: Effect of soil treatments on enzymatic activities in smelter/mines polluted soil.Data represent the mean of three replicates and error bars are standard deviations.

Mentions: Soil enzymes act as biological catalysts to facilitate biochemical reactions, organic matter decomposition and transformation; detoxify pollutants; produce essential compounds for microbes and plants; synthesize humus; and maintain soil structure642. β-glucosidase, alkaline phosphatase and urease were found in FC soil (Fig. 5). PTEs interact with the enzyme–substrate complex, denature the enzyme or interact with its active groups, as well as affect the synthesis of enzymes by microbial cells, which affects the enzymatic activity in soil33. Higher concentrations of PTEs result in a lower enzymatic activity, which reflects the pollution severity in soil. Enzyme-clay and enzyme-organic polymer complexes resist denaturation6.


Streptomyces pactum assisted phytoremediation in Zn/Pb smelter contaminated soil of Feng County and its impact on enzymatic activities
Effect of soil treatments on enzymatic activities in smelter/mines polluted soil.Data represent the mean of three replicates and error bars are standard deviations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384225&req=5

f5: Effect of soil treatments on enzymatic activities in smelter/mines polluted soil.Data represent the mean of three replicates and error bars are standard deviations.
Mentions: Soil enzymes act as biological catalysts to facilitate biochemical reactions, organic matter decomposition and transformation; detoxify pollutants; produce essential compounds for microbes and plants; synthesize humus; and maintain soil structure642. β-glucosidase, alkaline phosphatase and urease were found in FC soil (Fig. 5). PTEs interact with the enzyme–substrate complex, denature the enzyme or interact with its active groups, as well as affect the synthesis of enzymes by microbial cells, which affects the enzymatic activity in soil33. Higher concentrations of PTEs result in a lower enzymatic activity, which reflects the pollution severity in soil. Enzyme-clay and enzyme-organic polymer complexes resist denaturation6.

View Article: PubMed Central - PubMed

ABSTRACT

Anthropogenic activities, such as industrial expansion, smelting, mining and agricultural practices, have intensified the discharge of potentially toxic trace elements (PTEs) into the environment, threatening human health and other organisms. To assist phytoremediation by sorghum in soil contaminated by smelters/mines in Feng County (FC), a pot experiment was performed to examine the phytoremediation potential of Streptomyces pactum (Act12) + biochar. The results showed that root uptake of Zn and Cd was reduced by 45 and 22%, respectively, while the uptake of Pb and Cu increased by 17 and 47%, respectively. The shoot and root dry weight and chlorophyll content improved after Act12 inoculation. β-glucosidase, alkaline phosphatase and urease activities in soil improved and antioxidant activities (POD, PAL, PPO) decreased after application of Act12 + biochar due to a reduction in stress from PTEs. BCF, TF and MEA confirmed the role of Act12 in the amelioration and translocation of PTEs. PCA analysis showed a correlation between different factors that affect the translocation of PTEs. Overall, Act12 promoted the phytoremediation of PTEs. Field experiments on Act12 + biochar may provide new insights into the rehabilitation and restoration of soils contaminated by mines.

No MeSH data available.