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Effect of Leaves of Caesalpinia decapetala on Oxidative Stability of Oil-in-Water Emulsions

View Article: PubMed Central - PubMed

ABSTRACT

Caesalpinia decapetala (Roth) Alston (Fabaceae) (CD) is used in folk medicine to prevent colds and treat bronchitis. This plant has antitumor and antioxidant activity. The antioxidant effects of an extract from Caesalpinia decapetala (Fabaceae) were assessed by storage of model food oil-in-water emulsions with analysis of primary and secondary oxidation products. The antioxidant capacity of the plant extract was evaluated by the diphenylpicrylhydrazyl (DPPH), Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays and by electron paramagnetic resonance (EPR) spectroscopy. Lyophilized extracts of CD were added at concentrations of 0.002%, 0.02% and 0.2% into oil-in-water emulsions, which were stored for 30 days at 33 ± 1 °C, and then, oxidative stability was evaluated. The CD extract had high antioxidant activity (700 ± 70 µmol Trolox/g dry plant for the ORAC assay), mainly due to its phenolic components: gallic acid, quercetin, catechin, 4-hydroxybenzoic acid and p-coumaric acid. At a concentration of 0.2%, the extract significantly reduced the oxidative deterioration of oil-in-water emulsions. The results of the present study show the possibility of utilizing CD as a promising source of natural antioxidants for retarding lipid oxidation in the food and cosmetic industries.

No MeSH data available.


(a) Antioxidant activity determined by the electron paramagnetic resonance (EPR) assay for the CD (Caesalpinia decapetala) extract at different concentrations and (b) the correlation between the ORAC (oxygen radical absorbance capacity) and TEAC (trolox equivalent antioxidant capacity) assays with the EPR assay.
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antioxidants-06-00019-f002: (a) Antioxidant activity determined by the electron paramagnetic resonance (EPR) assay for the CD (Caesalpinia decapetala) extract at different concentrations and (b) the correlation between the ORAC (oxygen radical absorbance capacity) and TEAC (trolox equivalent antioxidant capacity) assays with the EPR assay.

Mentions: Figure 2 shows the decrease of arbitrary units with the increase of CD extract concentration. Due to its relatively short half-life, the methoxy radical (CH3O•) was identified by its ability to form a stable adduct with DMPO, the DMPO–OCH3. This compound can be detected by the double integration value of the EPR signal, from which the values of arbitrary units are obtained. The free radical scavenging activity of CD extracts was studied by competitive reaction in the presence of DMPO, which acted as spin trap, measured by EPR spectroscopy. The x-axis represents the plant extract concentration expressed as mg/L. The y-axis represents the arbitrary units obtained from the integrated EPR sign.


Effect of Leaves of Caesalpinia decapetala on Oxidative Stability of Oil-in-Water Emulsions
(a) Antioxidant activity determined by the electron paramagnetic resonance (EPR) assay for the CD (Caesalpinia decapetala) extract at different concentrations and (b) the correlation between the ORAC (oxygen radical absorbance capacity) and TEAC (trolox equivalent antioxidant capacity) assays with the EPR assay.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384182&req=5

antioxidants-06-00019-f002: (a) Antioxidant activity determined by the electron paramagnetic resonance (EPR) assay for the CD (Caesalpinia decapetala) extract at different concentrations and (b) the correlation between the ORAC (oxygen radical absorbance capacity) and TEAC (trolox equivalent antioxidant capacity) assays with the EPR assay.
Mentions: Figure 2 shows the decrease of arbitrary units with the increase of CD extract concentration. Due to its relatively short half-life, the methoxy radical (CH3O•) was identified by its ability to form a stable adduct with DMPO, the DMPO–OCH3. This compound can be detected by the double integration value of the EPR signal, from which the values of arbitrary units are obtained. The free radical scavenging activity of CD extracts was studied by competitive reaction in the presence of DMPO, which acted as spin trap, measured by EPR spectroscopy. The x-axis represents the plant extract concentration expressed as mg/L. The y-axis represents the arbitrary units obtained from the integrated EPR sign.

View Article: PubMed Central - PubMed

ABSTRACT

Caesalpinia decapetala (Roth) Alston (Fabaceae) (CD) is used in folk medicine to prevent colds and treat bronchitis. This plant has antitumor and antioxidant activity. The antioxidant effects of an extract from Caesalpinia decapetala (Fabaceae) were assessed by storage of model food oil-in-water emulsions with analysis of primary and secondary oxidation products. The antioxidant capacity of the plant extract was evaluated by the diphenylpicrylhydrazyl (DPPH), Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays and by electron paramagnetic resonance (EPR) spectroscopy. Lyophilized extracts of CD were added at concentrations of 0.002%, 0.02% and 0.2% into oil-in-water emulsions, which were stored for 30 days at 33 ± 1 °C, and then, oxidative stability was evaluated. The CD extract had high antioxidant activity (700 ± 70 µmol Trolox/g dry plant for the ORAC assay), mainly due to its phenolic components: gallic acid, quercetin, catechin, 4-hydroxybenzoic acid and p-coumaric acid. At a concentration of 0.2%, the extract significantly reduced the oxidative deterioration of oil-in-water emulsions. The results of the present study show the possibility of utilizing CD as a promising source of natural antioxidants for retarding lipid oxidation in the food and cosmetic industries.

No MeSH data available.