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Effect of Leaves of Caesalpinia decapetala on Oxidative Stability of Oil-in-Water Emulsions

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ABSTRACT

Caesalpinia decapetala (Roth) Alston (Fabaceae) (CD) is used in folk medicine to prevent colds and treat bronchitis. This plant has antitumor and antioxidant activity. The antioxidant effects of an extract from Caesalpinia decapetala (Fabaceae) were assessed by storage of model food oil-in-water emulsions with analysis of primary and secondary oxidation products. The antioxidant capacity of the plant extract was evaluated by the diphenylpicrylhydrazyl (DPPH), Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays and by electron paramagnetic resonance (EPR) spectroscopy. Lyophilized extracts of CD were added at concentrations of 0.002%, 0.02% and 0.2% into oil-in-water emulsions, which were stored for 30 days at 33 ± 1 °C, and then, oxidative stability was evaluated. The CD extract had high antioxidant activity (700 ± 70 µmol Trolox/g dry plant for the ORAC assay), mainly due to its phenolic components: gallic acid, quercetin, catechin, 4-hydroxybenzoic acid and p-coumaric acid. At a concentration of 0.2%, the extract significantly reduced the oxidative deterioration of oil-in-water emulsions. The results of the present study show the possibility of utilizing CD as a promising source of natural antioxidants for retarding lipid oxidation in the food and cosmetic industries.

No MeSH data available.


ESI (−)-MS/MS spectra corresponding to: catechin (a); quercetin (b); gallic acid (c); 4-hydroxybenzoic acid (d); and p-coumaric acid (e).
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antioxidants-06-00019-f001: ESI (−)-MS/MS spectra corresponding to: catechin (a); quercetin (b); gallic acid (c); 4-hydroxybenzoic acid (d); and p-coumaric acid (e).

Mentions: Figure 1 shows the ESI(–)-MS/MS spectra for the compounds. The analysis of the components in the CD extract showed that samples contained catechin (a), quercetin (b), gallic acid (c), 4-hydroxybenzoic acid (d) and p-coumaric (e). These results were obtained by comparison of spectra and retention times with reference standards. Table 2 shows the empirical formula, retention time and concentration of the compounds found in the CD extract. Gallic acid was present at the highest concentration (77,824 ng·mL−1), followed by catechin (669 ng·mL−1), p-coumaric acid and 4-hydroxybenzoic acid (155 ng·mL−1), and the lowest concentration was quercetin (64 ng·mL−1).


Effect of Leaves of Caesalpinia decapetala on Oxidative Stability of Oil-in-Water Emulsions
ESI (−)-MS/MS spectra corresponding to: catechin (a); quercetin (b); gallic acid (c); 4-hydroxybenzoic acid (d); and p-coumaric acid (e).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384182&req=5

antioxidants-06-00019-f001: ESI (−)-MS/MS spectra corresponding to: catechin (a); quercetin (b); gallic acid (c); 4-hydroxybenzoic acid (d); and p-coumaric acid (e).
Mentions: Figure 1 shows the ESI(–)-MS/MS spectra for the compounds. The analysis of the components in the CD extract showed that samples contained catechin (a), quercetin (b), gallic acid (c), 4-hydroxybenzoic acid (d) and p-coumaric (e). These results were obtained by comparison of spectra and retention times with reference standards. Table 2 shows the empirical formula, retention time and concentration of the compounds found in the CD extract. Gallic acid was present at the highest concentration (77,824 ng·mL−1), followed by catechin (669 ng·mL−1), p-coumaric acid and 4-hydroxybenzoic acid (155 ng·mL−1), and the lowest concentration was quercetin (64 ng·mL−1).

View Article: PubMed Central - PubMed

ABSTRACT

Caesalpinia decapetala (Roth) Alston (Fabaceae) (CD) is used in folk medicine to prevent colds and treat bronchitis. This plant has antitumor and antioxidant activity. The antioxidant effects of an extract from Caesalpinia decapetala (Fabaceae) were assessed by storage of model food oil-in-water emulsions with analysis of primary and secondary oxidation products. The antioxidant capacity of the plant extract was evaluated by the diphenylpicrylhydrazyl (DPPH), Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays and by electron paramagnetic resonance (EPR) spectroscopy. Lyophilized extracts of CD were added at concentrations of 0.002%, 0.02% and 0.2% into oil-in-water emulsions, which were stored for 30 days at 33 ± 1 °C, and then, oxidative stability was evaluated. The CD extract had high antioxidant activity (700 ± 70 µmol Trolox/g dry plant for the ORAC assay), mainly due to its phenolic components: gallic acid, quercetin, catechin, 4-hydroxybenzoic acid and p-coumaric acid. At a concentration of 0.2%, the extract significantly reduced the oxidative deterioration of oil-in-water emulsions. The results of the present study show the possibility of utilizing CD as a promising source of natural antioxidants for retarding lipid oxidation in the food and cosmetic industries.

No MeSH data available.