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Antioxidant Compound Extraction from Maqui ( Aristotelia chilensis [Mol] Stuntz) Berries: Optimization by Response Surface Methodology

View Article: PubMed Central

ABSTRACT

The optimum conditions for the antioxidant extraction from maqui berry were determined using a response surface methodology. A three level D-optimal design was used to investigate the effects of three independent variables namely, solvent type (methanol, acetone and ethanol), solvent concentration and extraction time over total antioxidant capacity by using the oxygen radical absorbance capacity (ORAC) method. The D-optimal design considered 42 experiments including 10 central point replicates. A second-order polynomial model showed that more than 89% of the variation is explained with a satisfactory prediction (78%). ORAC values are higher when acetone was used as a solvent at lower concentrations, and the extraction time range studied showed no significant influence on ORAC values. The optimal conditions for antioxidant extraction obtained were 29% of acetone for 159 min under agitation. From the results obtained it can be concluded that the given predictive model describes an antioxidant extraction process from maqui berry.

No MeSH data available.


Related in: MedlinePlus

Response surface maps plotting x2 solvent concentration vs. x3 extraction time, with (a) x1 methanol; (b) x1 ethanol; and (c) x1 acetone.
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antioxidants-06-00010-f003: Response surface maps plotting x2 solvent concentration vs. x3 extraction time, with (a) x1 methanol; (b) x1 ethanol; and (c) x1 acetone.

Mentions: The large and negative quadratic regression coefficient b22 for concentration (green bar in Figure 2) indicates a strong curvature in the response for the antioxidant capacity (Figure 3), in which a maximum ORAC value can be reached within the experimental region. Regarding to solvent type, it is observed that only acetone has a positive effect in ORAC value (light blue bar in Figure 2). Thus, antioxidant compounds appear to be more extractable in acetone than in methanol or ethanol. C├ęspedes et al. [5] indicated that in maqui extract the acetone partition showed an excellent antioxidant activity.


Antioxidant Compound Extraction from Maqui ( Aristotelia chilensis [Mol] Stuntz) Berries: Optimization by Response Surface Methodology
Response surface maps plotting x2 solvent concentration vs. x3 extraction time, with (a) x1 methanol; (b) x1 ethanol; and (c) x1 acetone.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384173&req=5

antioxidants-06-00010-f003: Response surface maps plotting x2 solvent concentration vs. x3 extraction time, with (a) x1 methanol; (b) x1 ethanol; and (c) x1 acetone.
Mentions: The large and negative quadratic regression coefficient b22 for concentration (green bar in Figure 2) indicates a strong curvature in the response for the antioxidant capacity (Figure 3), in which a maximum ORAC value can be reached within the experimental region. Regarding to solvent type, it is observed that only acetone has a positive effect in ORAC value (light blue bar in Figure 2). Thus, antioxidant compounds appear to be more extractable in acetone than in methanol or ethanol. C├ęspedes et al. [5] indicated that in maqui extract the acetone partition showed an excellent antioxidant activity.

View Article: PubMed Central

ABSTRACT

The optimum conditions for the antioxidant extraction from maqui berry were determined using a response surface methodology. A three level D-optimal design was used to investigate the effects of three independent variables namely, solvent type (methanol, acetone and ethanol), solvent concentration and extraction time over total antioxidant capacity by using the oxygen radical absorbance capacity (ORAC) method. The D-optimal design considered 42 experiments including 10 central point replicates. A second-order polynomial model showed that more than 89% of the variation is explained with a satisfactory prediction (78%). ORAC values are higher when acetone was used as a solvent at lower concentrations, and the extraction time range studied showed no significant influence on ORAC values. The optimal conditions for antioxidant extraction obtained were 29% of acetone for 159 min under agitation. From the results obtained it can be concluded that the given predictive model describes an antioxidant extraction process from maqui berry.

No MeSH data available.


Related in: MedlinePlus