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The Effects of Allicin, a Reactive Sulfur Species from Garlic, on a Selection of Mammalian Cell Lines

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ABSTRACT

Garlic (Allium sativum L.) has been used as a spice and medicinal plant since ancient times. Garlic produces the thiol-reactive defence substance, allicin, upon wounding. The effects of allicin on human lung epithelium carcinoma (A549), mouse fibroblast (3T3), human umbilical vein endothelial cell (HUVEC), human colon carcinoma (HT29) and human breast cancer (MCF7) cell lines were tested. To estimate toxic effects of allicin, we used a standard MTT-test (methylthiazoltetrazolium) for cell viability and 3H-thymidine incorporation for cell proliferation. The glutathione pool was measured using monobromobimane and the formation of reactive species was identified using 2′,7′-dichlorofluoresceine-diacetate. The YO-PRO-1 iodide staining procedure was used to estimate apoptosis. Allicin reduced cell viability and cell proliferation in a concentration dependent manner. In the bimane test, it was observed that cells treated with allicin showed reduced fluorescence, suggesting glutathione oxidation. The cell lines tested differed in sensitivity to allicin in regard to viability, cell proliferation and glutathione oxidation. The 3T3 and MCF-7 cells showed a higher proportion of apoptosis compared to the other cell types. These data show that mammalian cell lines differ in their sensitivity and responses to allicin.

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Effect of garlic juice normalized to its allicin concentration (A); and synthetic allicin (B) on cell proliferation as tested by incorporation of 3H labelled thymidine in DNA and measured by scintillation. All data points are means of three replicates; error bars show standard deviation.
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antioxidants-06-00001-f002: Effect of garlic juice normalized to its allicin concentration (A); and synthetic allicin (B) on cell proliferation as tested by incorporation of 3H labelled thymidine in DNA and measured by scintillation. All data points are means of three replicates; error bars show standard deviation.

Mentions: The cell lines tested proliferated at different rates even in the untreated controls (Figure 2A,B). Nevertheless, allicin inhibited cell proliferation of all cell types in a concentration dependent manner, with the respective cell lines showing differential sensitivities. HUVEC cells were the most sensitive to allicin, with the incorporation of 3H-thymidine into newly synthesized DNA markedly reduced at 0.0094 mM allicin in garlic juice (p > 0.05, one-way-AnovaR, Holm–Sidak method) and completely inhibited at 0.0188–0.0375 mM allicin respectively (Figure 2A,B, p > 0.05, one-way-AnovaR, Holm–Sidak method). Mouse fibroblast 3T3 cells were the least sensitive, still showing normal 3H-thymidine incorporation at 0.0375 mM allicin where other cell lines were inactive (Figure 2A,B, p > 0.05, one-way-AnovaR, Holm–Sidak method). Human colorectal epithelial cells (HT29), human mammary carcinoma cells (MCF7) and lung epithelial carcinoma cells (A549) showed intermediate behaviour (Figure 2A,B).


The Effects of Allicin, a Reactive Sulfur Species from Garlic, on a Selection of Mammalian Cell Lines
Effect of garlic juice normalized to its allicin concentration (A); and synthetic allicin (B) on cell proliferation as tested by incorporation of 3H labelled thymidine in DNA and measured by scintillation. All data points are means of three replicates; error bars show standard deviation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384165&req=5

antioxidants-06-00001-f002: Effect of garlic juice normalized to its allicin concentration (A); and synthetic allicin (B) on cell proliferation as tested by incorporation of 3H labelled thymidine in DNA and measured by scintillation. All data points are means of three replicates; error bars show standard deviation.
Mentions: The cell lines tested proliferated at different rates even in the untreated controls (Figure 2A,B). Nevertheless, allicin inhibited cell proliferation of all cell types in a concentration dependent manner, with the respective cell lines showing differential sensitivities. HUVEC cells were the most sensitive to allicin, with the incorporation of 3H-thymidine into newly synthesized DNA markedly reduced at 0.0094 mM allicin in garlic juice (p > 0.05, one-way-AnovaR, Holm–Sidak method) and completely inhibited at 0.0188–0.0375 mM allicin respectively (Figure 2A,B, p > 0.05, one-way-AnovaR, Holm–Sidak method). Mouse fibroblast 3T3 cells were the least sensitive, still showing normal 3H-thymidine incorporation at 0.0375 mM allicin where other cell lines were inactive (Figure 2A,B, p > 0.05, one-way-AnovaR, Holm–Sidak method). Human colorectal epithelial cells (HT29), human mammary carcinoma cells (MCF7) and lung epithelial carcinoma cells (A549) showed intermediate behaviour (Figure 2A,B).

View Article: PubMed Central - PubMed

ABSTRACT

Garlic (Allium sativum L.) has been used as a spice and medicinal plant since ancient times. Garlic produces the thiol-reactive defence substance, allicin, upon wounding. The effects of allicin on human lung epithelium carcinoma (A549), mouse fibroblast (3T3), human umbilical vein endothelial cell (HUVEC), human colon carcinoma (HT29) and human breast cancer (MCF7) cell lines were tested. To estimate toxic effects of allicin, we used a standard MTT-test (methylthiazoltetrazolium) for cell viability and 3H-thymidine incorporation for cell proliferation. The glutathione pool was measured using monobromobimane and the formation of reactive species was identified using 2′,7′-dichlorofluoresceine-diacetate. The YO-PRO-1 iodide staining procedure was used to estimate apoptosis. Allicin reduced cell viability and cell proliferation in a concentration dependent manner. In the bimane test, it was observed that cells treated with allicin showed reduced fluorescence, suggesting glutathione oxidation. The cell lines tested differed in sensitivity to allicin in regard to viability, cell proliferation and glutathione oxidation. The 3T3 and MCF-7 cells showed a higher proportion of apoptosis compared to the other cell types. These data show that mammalian cell lines differ in their sensitivity and responses to allicin.

No MeSH data available.


Related in: MedlinePlus