Limits...
The Effects of Allicin, a Reactive Sulfur Species from Garlic, on a Selection of Mammalian Cell Lines

View Article: PubMed Central - PubMed

ABSTRACT

Garlic (Allium sativum L.) has been used as a spice and medicinal plant since ancient times. Garlic produces the thiol-reactive defence substance, allicin, upon wounding. The effects of allicin on human lung epithelium carcinoma (A549), mouse fibroblast (3T3), human umbilical vein endothelial cell (HUVEC), human colon carcinoma (HT29) and human breast cancer (MCF7) cell lines were tested. To estimate toxic effects of allicin, we used a standard MTT-test (methylthiazoltetrazolium) for cell viability and 3H-thymidine incorporation for cell proliferation. The glutathione pool was measured using monobromobimane and the formation of reactive species was identified using 2′,7′-dichlorofluoresceine-diacetate. The YO-PRO-1 iodide staining procedure was used to estimate apoptosis. Allicin reduced cell viability and cell proliferation in a concentration dependent manner. In the bimane test, it was observed that cells treated with allicin showed reduced fluorescence, suggesting glutathione oxidation. The cell lines tested differed in sensitivity to allicin in regard to viability, cell proliferation and glutathione oxidation. The 3T3 and MCF-7 cells showed a higher proportion of apoptosis compared to the other cell types. These data show that mammalian cell lines differ in their sensitivity and responses to allicin.

No MeSH data available.


Effect of garlic juice normalized to its allicin concentration (A); and synthetic allicin (B) on the viability of different cell lines in the MTT (methylthiazoltetrazolium)-assay. All data points are means of three replicates; error bars show standard deviation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5384165&req=5

antioxidants-06-00001-f001: Effect of garlic juice normalized to its allicin concentration (A); and synthetic allicin (B) on the viability of different cell lines in the MTT (methylthiazoltetrazolium)-assay. All data points are means of three replicates; error bars show standard deviation.

Mentions: Cell viability, in terms of MTT reduction, decreased in a concentration dependent manner for both garlic juice- and synthetic allicin-treated samples in an essentially similar manner (Figure 1). Of the cell lines tested, human endothelial vein cells (HUVEC) were most sensitive to allicin. HUVEC cell viability decreased by approximately 50% at 0.0094–0.0188 mM allicin (Figure 1A,B). There was a reduction in HUVEC cell viability at 0.0094–0.0188 mM allicin in garlic juice and 0.0188 mM synthetic allicin was significant compared to the other cell lines (p > 0.05, one-way-AnovaR, Holm–Sidak method). Human adenocarcinoma (A549), mouse fibroblast (3T3) and human mammary carcinoma (MCF7) cells were most resistant and show a decrease of 50% MTT absorption between 0.0375 and 0.075 mM allicin. These three cell types were significantly more resistant to 0.0375 mM allicin than the Human colorectal epithelial cells (HT29) (p > 0.05, one-way-AnovaR, Holm–Sidak method). HT29 cells were intermediate in sensitivity, showing approximately 50% reduction in viability at 0.188–0.375 mM allicin. This demonstrates that different cell-lines show intrinsically different sensitivity to allicin. The slight increase in A550 at higher concentrations of garlic juice (0.6 and 1.2 mM) was an artefact due to increasing turbidity of the solution and can be seen in all cell lines. The ranking of differential sensitivities of the cell lines to synthetic allicin or to allicin in garlic juice was the same i.e., HUVEC > HT29 > A549, 3T3 and MCF7 (Figure 1A,B).


The Effects of Allicin, a Reactive Sulfur Species from Garlic, on a Selection of Mammalian Cell Lines
Effect of garlic juice normalized to its allicin concentration (A); and synthetic allicin (B) on the viability of different cell lines in the MTT (methylthiazoltetrazolium)-assay. All data points are means of three replicates; error bars show standard deviation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384165&req=5

antioxidants-06-00001-f001: Effect of garlic juice normalized to its allicin concentration (A); and synthetic allicin (B) on the viability of different cell lines in the MTT (methylthiazoltetrazolium)-assay. All data points are means of three replicates; error bars show standard deviation.
Mentions: Cell viability, in terms of MTT reduction, decreased in a concentration dependent manner for both garlic juice- and synthetic allicin-treated samples in an essentially similar manner (Figure 1). Of the cell lines tested, human endothelial vein cells (HUVEC) were most sensitive to allicin. HUVEC cell viability decreased by approximately 50% at 0.0094–0.0188 mM allicin (Figure 1A,B). There was a reduction in HUVEC cell viability at 0.0094–0.0188 mM allicin in garlic juice and 0.0188 mM synthetic allicin was significant compared to the other cell lines (p > 0.05, one-way-AnovaR, Holm–Sidak method). Human adenocarcinoma (A549), mouse fibroblast (3T3) and human mammary carcinoma (MCF7) cells were most resistant and show a decrease of 50% MTT absorption between 0.0375 and 0.075 mM allicin. These three cell types were significantly more resistant to 0.0375 mM allicin than the Human colorectal epithelial cells (HT29) (p > 0.05, one-way-AnovaR, Holm–Sidak method). HT29 cells were intermediate in sensitivity, showing approximately 50% reduction in viability at 0.188–0.375 mM allicin. This demonstrates that different cell-lines show intrinsically different sensitivity to allicin. The slight increase in A550 at higher concentrations of garlic juice (0.6 and 1.2 mM) was an artefact due to increasing turbidity of the solution and can be seen in all cell lines. The ranking of differential sensitivities of the cell lines to synthetic allicin or to allicin in garlic juice was the same i.e., HUVEC > HT29 > A549, 3T3 and MCF7 (Figure 1A,B).

View Article: PubMed Central - PubMed

ABSTRACT

Garlic (Allium sativum L.) has been used as a spice and medicinal plant since ancient times. Garlic produces the thiol-reactive defence substance, allicin, upon wounding. The effects of allicin on human lung epithelium carcinoma (A549), mouse fibroblast (3T3), human umbilical vein endothelial cell (HUVEC), human colon carcinoma (HT29) and human breast cancer (MCF7) cell lines were tested. To estimate toxic effects of allicin, we used a standard MTT-test (methylthiazoltetrazolium) for cell viability and 3H-thymidine incorporation for cell proliferation. The glutathione pool was measured using monobromobimane and the formation of reactive species was identified using 2′,7′-dichlorofluoresceine-diacetate. The YO-PRO-1 iodide staining procedure was used to estimate apoptosis. Allicin reduced cell viability and cell proliferation in a concentration dependent manner. In the bimane test, it was observed that cells treated with allicin showed reduced fluorescence, suggesting glutathione oxidation. The cell lines tested differed in sensitivity to allicin in regard to viability, cell proliferation and glutathione oxidation. The 3T3 and MCF-7 cells showed a higher proportion of apoptosis compared to the other cell types. These data show that mammalian cell lines differ in their sensitivity and responses to allicin.

No MeSH data available.