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Evaluation of antigen-induced synovitis in a porcine model: Immunological, arthroscopic and kinetic studies

View Article: PubMed Central - PubMed

ABSTRACT

Background: Synovitis is an inflammation-related disease linked to rheumatoid arthritis, osteoarthritis, infections and trauma. This inflammation is accompanied by immune cells infiltration which initiates an inflammatory response causing pain, discomfort and affecting the normal joint function. The treatment of synovitis is based on the administration of anti-inflammatory drugs or biological agents such as platelet rich plasma and mesenchymal stem cells. However, the evaluation and validation of more effective therapies of synovitis requires the establishment of clinically relevant animal models.

Results: In this study, Large White pigs were pre-immunized to evaluate an antigen-induced synovitis. The immune monitoring of synovial fluids in this model allowed us the identification of IL-12p40 and T cell subsets as immune biomarkers. Moreover, the evolution of synovitis was performed by arthroscopic procedures and kinetic analysis. In summary, this paper describes an animal model of antigen-induced synovitis to be used in the evaluation of anti-inflammatory therapies.

Conclusions: The novelty of this paper lies in the development of a clinically relevant model of synovitis which permits the simultaneous evaluation of synovitis from an immunological, surgical and kinetic point of view.

Electronic supplementary material: The online version of this article (doi:10.1186/s12917-017-1025-4) contains supplementary material, which is available to authorized users.

No MeSH data available.


Pressure platform gait analysis. Seven days after intra-articular injection of PBS or BSA, a pressure platform gait analysis was performed to evaluate plantar pressure distributions. a Above, a representative image of the gait analysis (LF: left forelimb; LH: left hind limb; RF: right forelimb; RH: right hind limb) is represented. Below, the pressure of each limb is shown. The legend on the right shows the equivalence between numeric and colorimetric values. Maximum forces b and impulses c in control and BSA-injected limbs (n = 4). The lower boundary of the box indicates the 25th percentile and the upper boundary the 75th percentile. Bars above and below the box indicate the 90th and 10th percentiles. The line within the box marks the median. No significant differences were found between PBS and BSA injected joints
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Fig7: Pressure platform gait analysis. Seven days after intra-articular injection of PBS or BSA, a pressure platform gait analysis was performed to evaluate plantar pressure distributions. a Above, a representative image of the gait analysis (LF: left forelimb; LH: left hind limb; RF: right forelimb; RH: right hind limb) is represented. Below, the pressure of each limb is shown. The legend on the right shows the equivalence between numeric and colorimetric values. Maximum forces b and impulses c in control and BSA-injected limbs (n = 4). The lower boundary of the box indicates the 25th percentile and the upper boundary the 75th percentile. Bars above and below the box indicate the 90th and 10th percentiles. The line within the box marks the median. No significant differences were found between PBS and BSA injected joints

Mentions: The kinematic gait parameters were evaluated by a pressure platform. The pre-sensitized animals were biomechanically evaluated at day 7 after intra-articular injections of BSA or PBS. The parameters evaluated were impulse (Kg x sec) and the vertical maximum force (Kg). In terms of animal management, our experience demonstrated that kinematic parameters could be easily quantified with the porcine model (Fig. 7a). Our results showed that, the impulses in the forelimbs with BSA showed an enormous inter-individual variability (Fig. 1c) and no significant difference was observed in terms of vertical maximum force (Fig. 7b). Finally, it is important to note that because of ethics and animal welfare, this kinematic analysis had to be performed under analgesia.Fig. 7


Evaluation of antigen-induced synovitis in a porcine model: Immunological, arthroscopic and kinetic studies
Pressure platform gait analysis. Seven days after intra-articular injection of PBS or BSA, a pressure platform gait analysis was performed to evaluate plantar pressure distributions. a Above, a representative image of the gait analysis (LF: left forelimb; LH: left hind limb; RF: right forelimb; RH: right hind limb) is represented. Below, the pressure of each limb is shown. The legend on the right shows the equivalence between numeric and colorimetric values. Maximum forces b and impulses c in control and BSA-injected limbs (n = 4). The lower boundary of the box indicates the 25th percentile and the upper boundary the 75th percentile. Bars above and below the box indicate the 90th and 10th percentiles. The line within the box marks the median. No significant differences were found between PBS and BSA injected joints
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5384159&req=5

Fig7: Pressure platform gait analysis. Seven days after intra-articular injection of PBS or BSA, a pressure platform gait analysis was performed to evaluate plantar pressure distributions. a Above, a representative image of the gait analysis (LF: left forelimb; LH: left hind limb; RF: right forelimb; RH: right hind limb) is represented. Below, the pressure of each limb is shown. The legend on the right shows the equivalence between numeric and colorimetric values. Maximum forces b and impulses c in control and BSA-injected limbs (n = 4). The lower boundary of the box indicates the 25th percentile and the upper boundary the 75th percentile. Bars above and below the box indicate the 90th and 10th percentiles. The line within the box marks the median. No significant differences were found between PBS and BSA injected joints
Mentions: The kinematic gait parameters were evaluated by a pressure platform. The pre-sensitized animals were biomechanically evaluated at day 7 after intra-articular injections of BSA or PBS. The parameters evaluated were impulse (Kg x sec) and the vertical maximum force (Kg). In terms of animal management, our experience demonstrated that kinematic parameters could be easily quantified with the porcine model (Fig. 7a). Our results showed that, the impulses in the forelimbs with BSA showed an enormous inter-individual variability (Fig. 1c) and no significant difference was observed in terms of vertical maximum force (Fig. 7b). Finally, it is important to note that because of ethics and animal welfare, this kinematic analysis had to be performed under analgesia.Fig. 7

View Article: PubMed Central - PubMed

ABSTRACT

Background: Synovitis is an inflammation-related disease linked to rheumatoid arthritis, osteoarthritis, infections and trauma. This inflammation is accompanied by immune cells infiltration which initiates an inflammatory response causing pain, discomfort and affecting the normal joint function. The treatment of synovitis is based on the administration of anti-inflammatory drugs or biological agents such as platelet rich plasma and mesenchymal stem cells. However, the evaluation and validation of more effective therapies of synovitis requires the establishment of clinically relevant animal models.

Results: In this study, Large White pigs were pre-immunized to evaluate an antigen-induced synovitis. The immune monitoring of synovial fluids in this model allowed us the identification of IL-12p40 and T cell subsets as immune biomarkers. Moreover, the evolution of synovitis was performed by arthroscopic procedures and kinetic analysis. In summary, this paper describes an animal model of antigen-induced synovitis to be used in the evaluation of anti-inflammatory therapies.

Conclusions: The novelty of this paper lies in the development of a clinically relevant model of synovitis which permits the simultaneous evaluation of synovitis from an immunological, surgical and kinetic point of view.

Electronic supplementary material: The online version of this article (doi:10.1186/s12917-017-1025-4) contains supplementary material, which is available to authorized users.

No MeSH data available.