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Genome-wide identification and expression analysis of ClLAX , ClPIN and ClABCB genes families in Citrullus lanatus under various abiotic stresses and grafting

View Article: PubMed Central - PubMed

ABSTRACT

Background: Auxin plays an important role in regulating plant growth and development as well as in the response of plants to abiotic stresses. Auxin is transported by three kinds of major protein families, including the AUXIN RESISTANT 1/LIKE AUX1 (AUX⁄LAX) influx carriers, the PIN-FORMED (PIN) efflux carriers and the ATP binding cassette B/P-glycoprotein/Multidrug-resistance (ABCB/MDR/PGP) efflux/condition carriers. The biological function of several auxin transporter genes has been well characterized in Arabidopsis thaliana. However, their function in response to exogenous auxin and abiotic stresses in watermelon (Citrullus lanatus. L) remained unknown.

Results: Here, the latest updated watermelon genome was used to characterise the ClLAX, ClPIN and ClABCB family genes from watermelon. The genome-wide analysis of the ClLAX, ClPIN and ClABCB family genes, including chromosome localisation, gene structure, and phylogenic relationships, was carried out. Seven ClLAXs, 11 ClPINs and 15 ClABCBs were mapped on 10 watermelon chromosomes. The expression profiles of the ClLAX, ClPIN and ClABCB genes under exogenous indole-3-acetic acid and various abiotic stresses (salt, drought, and cold stresses) treatments were performed by quantitative real-time PCR (qRT-PCR). The transcriptional level of majority ClLAX, ClPIN and ClABCB genes were changed by abiotic stresses in both shoots and roots. We also analysed the expression levels of ClLAX, ClPIN and ClABCB genes in graft response.

Conclusion: Analysis of the expression patterns of ClLAX, ClPIN and ClABCB genes under salt, drought, cold treatment and grafting response helps us to understand the possible roles of auxin transporter genes in watermelon adaptation to environmental stresses.

Electronic supplementary material: The online version of this article (doi:10.1186/s12863-017-0500-z) contains supplementary material, which is available to authorized users.

No MeSH data available.


qRT-PCR analysis of ClLAX, ClPIN and ClABCB family genes in grafting-responsive
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Fig8: qRT-PCR analysis of ClLAX, ClPIN and ClABCB family genes in grafting-responsive

Mentions: Grafting is an ancient technique that is widely used in agriculture practices to improve productivity and stress resistance [53]. Auxin can increase the activity of cell division and wound healing in cut Arabidopsis inflorescence stems. However, the molecular mechanisms of auxin involved in these processes remain largely unclear. To investigate whether auxin transporter genes from watermelon are involved in grafting response, we analysed the expression profiles of ClLAX, ClPIN and ClABCB during grafting for 5 days in the shoots (Fig. 8). The data indicated that most of the ClLAX genes were downregulated and most of the ClPIN genes were upregulated in the shoots during grafting. Only ClABCB1, −7, −11 and −4 were down-regulated. The rest of the ClABCB family genes were significantly upregulated.Fig. 8


Genome-wide identification and expression analysis of ClLAX , ClPIN and ClABCB genes families in Citrullus lanatus under various abiotic stresses and grafting
qRT-PCR analysis of ClLAX, ClPIN and ClABCB family genes in grafting-responsive
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5384148&req=5

Fig8: qRT-PCR analysis of ClLAX, ClPIN and ClABCB family genes in grafting-responsive
Mentions: Grafting is an ancient technique that is widely used in agriculture practices to improve productivity and stress resistance [53]. Auxin can increase the activity of cell division and wound healing in cut Arabidopsis inflorescence stems. However, the molecular mechanisms of auxin involved in these processes remain largely unclear. To investigate whether auxin transporter genes from watermelon are involved in grafting response, we analysed the expression profiles of ClLAX, ClPIN and ClABCB during grafting for 5 days in the shoots (Fig. 8). The data indicated that most of the ClLAX genes were downregulated and most of the ClPIN genes were upregulated in the shoots during grafting. Only ClABCB1, −7, −11 and −4 were down-regulated. The rest of the ClABCB family genes were significantly upregulated.Fig. 8

View Article: PubMed Central - PubMed

ABSTRACT

Background: Auxin plays an important role in regulating plant growth and development as well as in the response of plants to abiotic stresses. Auxin is transported by three kinds of major protein families, including the AUXIN RESISTANT 1/LIKE AUX1 (AUX⁄LAX) influx carriers, the PIN-FORMED (PIN) efflux carriers and the ATP binding cassette B/P-glycoprotein/Multidrug-resistance (ABCB/MDR/PGP) efflux/condition carriers. The biological function of several auxin transporter genes has been well characterized in Arabidopsis thaliana. However, their function in response to exogenous auxin and abiotic stresses in watermelon (Citrullus lanatus. L) remained unknown.

Results: Here, the latest updated watermelon genome was used to characterise the ClLAX, ClPIN and ClABCB family genes from watermelon. The genome-wide analysis of the ClLAX, ClPIN and ClABCB family genes, including chromosome localisation, gene structure, and phylogenic relationships, was carried out. Seven ClLAXs, 11 ClPINs and 15 ClABCBs were mapped on 10 watermelon chromosomes. The expression profiles of the ClLAX, ClPIN and ClABCB genes under exogenous indole-3-acetic acid and various abiotic stresses (salt, drought, and cold stresses) treatments were performed by quantitative real-time PCR (qRT-PCR). The transcriptional level of majority ClLAX, ClPIN and ClABCB genes were changed by abiotic stresses in both shoots and roots. We also analysed the expression levels of ClLAX, ClPIN and ClABCB genes in graft response.

Conclusion: Analysis of the expression patterns of ClLAX, ClPIN and ClABCB genes under salt, drought, cold treatment and grafting response helps us to understand the possible roles of auxin transporter genes in watermelon adaptation to environmental stresses.

Electronic supplementary material: The online version of this article (doi:10.1186/s12863-017-0500-z) contains supplementary material, which is available to authorized users.

No MeSH data available.