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Genome-wide identification and expression analysis of ClLAX , ClPIN and ClABCB genes families in Citrullus lanatus under various abiotic stresses and grafting

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ABSTRACT

Background: Auxin plays an important role in regulating plant growth and development as well as in the response of plants to abiotic stresses. Auxin is transported by three kinds of major protein families, including the AUXIN RESISTANT 1/LIKE AUX1 (AUX⁄LAX) influx carriers, the PIN-FORMED (PIN) efflux carriers and the ATP binding cassette B/P-glycoprotein/Multidrug-resistance (ABCB/MDR/PGP) efflux/condition carriers. The biological function of several auxin transporter genes has been well characterized in Arabidopsis thaliana. However, their function in response to exogenous auxin and abiotic stresses in watermelon (Citrullus lanatus. L) remained unknown.

Results: Here, the latest updated watermelon genome was used to characterise the ClLAX, ClPIN and ClABCB family genes from watermelon. The genome-wide analysis of the ClLAX, ClPIN and ClABCB family genes, including chromosome localisation, gene structure, and phylogenic relationships, was carried out. Seven ClLAXs, 11 ClPINs and 15 ClABCBs were mapped on 10 watermelon chromosomes. The expression profiles of the ClLAX, ClPIN and ClABCB genes under exogenous indole-3-acetic acid and various abiotic stresses (salt, drought, and cold stresses) treatments were performed by quantitative real-time PCR (qRT-PCR). The transcriptional level of majority ClLAX, ClPIN and ClABCB genes were changed by abiotic stresses in both shoots and roots. We also analysed the expression levels of ClLAX, ClPIN and ClABCB genes in graft response.

Conclusion: Analysis of the expression patterns of ClLAX, ClPIN and ClABCB genes under salt, drought, cold treatment and grafting response helps us to understand the possible roles of auxin transporter genes in watermelon adaptation to environmental stresses.

Electronic supplementary material: The online version of this article (doi:10.1186/s12863-017-0500-z) contains supplementary material, which is available to authorized users.

No MeSH data available.


Expression profiles analysis of ClLAX, ClPIN and ClABCB family genes under IAA treatment in shoots (a) and roots (b). Total RNA was extracted from the shoots and roots of 3-week-old watermelon seedlings for expression analysis. The histogram shows the relative expression levels of ClLAX, ClPIN and ClABCB genes under IAA (10 μM) treatment compared to the untreated expression level. The ClACTIN (Cla004014) was used as internal standards. The relative expression levels were normalized to a value of 1 in mock seedlings
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Fig4: Expression profiles analysis of ClLAX, ClPIN and ClABCB family genes under IAA treatment in shoots (a) and roots (b). Total RNA was extracted from the shoots and roots of 3-week-old watermelon seedlings for expression analysis. The histogram shows the relative expression levels of ClLAX, ClPIN and ClABCB genes under IAA (10 μM) treatment compared to the untreated expression level. The ClACTIN (Cla004014) was used as internal standards. The relative expression levels were normalized to a value of 1 in mock seedlings

Mentions: Auxin regulating plant growth and development depends mainly on auxin transporter to regulate auxin relocation and homeostasis [23, 24]. Exogenous auxin treatment could accelerate or block the endogenous auxin transport between different tissues [23, 57]. To investigate whether the auxin transporters in watermelon were regulated by auxin, the expression profiles of ClLAX, ClPIN and ClABCB genes under 10 μM IAA for 9 h in the shoots and roots were analysed by qRT-PCR (Fig. 4). Total RNA was isolated from the shoots and roots of mock seedlings or IAA-treated seedlings at different time points (6, 12 and 24 h). Our data suggested that most ClLAX, ClPIN and ClABCB genes were auxin responsive genes. The majority of these genes were differentially regulated by IAA at the transcriptional level. IAA treatment increased the expression levels of ClLAX1, −7, ClPIN3,−4, −5, −6, −7 and ClABCB4 in the shoots more than five-fold. On the contrary, ClABCB1, −2,−5,−10 and −14 expression levels were downregulated in the shoots after IAA treatment (Fig. 4a). Most of the auxin transporter genes were upregulated after IAA treatment in the roots (Fig. 4b). IAA treatment upregulated the expression levels of ClLAX1, −2, −3, ClPIN3, −7, ClABCB2, −10 and −12 more than15-fold in the roots. In both the roots and shoots, the expression of ClABCB5 was down-regulated by IAA treatment.Fig. 4


Genome-wide identification and expression analysis of ClLAX , ClPIN and ClABCB genes families in Citrullus lanatus under various abiotic stresses and grafting
Expression profiles analysis of ClLAX, ClPIN and ClABCB family genes under IAA treatment in shoots (a) and roots (b). Total RNA was extracted from the shoots and roots of 3-week-old watermelon seedlings for expression analysis. The histogram shows the relative expression levels of ClLAX, ClPIN and ClABCB genes under IAA (10 μM) treatment compared to the untreated expression level. The ClACTIN (Cla004014) was used as internal standards. The relative expression levels were normalized to a value of 1 in mock seedlings
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5384148&req=5

Fig4: Expression profiles analysis of ClLAX, ClPIN and ClABCB family genes under IAA treatment in shoots (a) and roots (b). Total RNA was extracted from the shoots and roots of 3-week-old watermelon seedlings for expression analysis. The histogram shows the relative expression levels of ClLAX, ClPIN and ClABCB genes under IAA (10 μM) treatment compared to the untreated expression level. The ClACTIN (Cla004014) was used as internal standards. The relative expression levels were normalized to a value of 1 in mock seedlings
Mentions: Auxin regulating plant growth and development depends mainly on auxin transporter to regulate auxin relocation and homeostasis [23, 24]. Exogenous auxin treatment could accelerate or block the endogenous auxin transport between different tissues [23, 57]. To investigate whether the auxin transporters in watermelon were regulated by auxin, the expression profiles of ClLAX, ClPIN and ClABCB genes under 10 μM IAA for 9 h in the shoots and roots were analysed by qRT-PCR (Fig. 4). Total RNA was isolated from the shoots and roots of mock seedlings or IAA-treated seedlings at different time points (6, 12 and 24 h). Our data suggested that most ClLAX, ClPIN and ClABCB genes were auxin responsive genes. The majority of these genes were differentially regulated by IAA at the transcriptional level. IAA treatment increased the expression levels of ClLAX1, −7, ClPIN3,−4, −5, −6, −7 and ClABCB4 in the shoots more than five-fold. On the contrary, ClABCB1, −2,−5,−10 and −14 expression levels were downregulated in the shoots after IAA treatment (Fig. 4a). Most of the auxin transporter genes were upregulated after IAA treatment in the roots (Fig. 4b). IAA treatment upregulated the expression levels of ClLAX1, −2, −3, ClPIN3, −7, ClABCB2, −10 and −12 more than15-fold in the roots. In both the roots and shoots, the expression of ClABCB5 was down-regulated by IAA treatment.Fig. 4

View Article: PubMed Central - PubMed

ABSTRACT

Background: Auxin plays an important role in regulating plant growth and development as well as in the response of plants to abiotic stresses. Auxin is transported by three kinds of major protein families, including the AUXIN RESISTANT 1/LIKE AUX1 (AUX⁄LAX) influx carriers, the PIN-FORMED (PIN) efflux carriers and the ATP binding cassette B/P-glycoprotein/Multidrug-resistance (ABCB/MDR/PGP) efflux/condition carriers. The biological function of several auxin transporter genes has been well characterized in Arabidopsis thaliana. However, their function in response to exogenous auxin and abiotic stresses in watermelon (Citrullus lanatus. L) remained unknown.

Results: Here, the latest updated watermelon genome was used to characterise the ClLAX, ClPIN and ClABCB family genes from watermelon. The genome-wide analysis of the ClLAX, ClPIN and ClABCB family genes, including chromosome localisation, gene structure, and phylogenic relationships, was carried out. Seven ClLAXs, 11 ClPINs and 15 ClABCBs were mapped on 10 watermelon chromosomes. The expression profiles of the ClLAX, ClPIN and ClABCB genes under exogenous indole-3-acetic acid and various abiotic stresses (salt, drought, and cold stresses) treatments were performed by quantitative real-time PCR (qRT-PCR). The transcriptional level of majority ClLAX, ClPIN and ClABCB genes were changed by abiotic stresses in both shoots and roots. We also analysed the expression levels of ClLAX, ClPIN and ClABCB genes in graft response.

Conclusion: Analysis of the expression patterns of ClLAX, ClPIN and ClABCB genes under salt, drought, cold treatment and grafting response helps us to understand the possible roles of auxin transporter genes in watermelon adaptation to environmental stresses.

Electronic supplementary material: The online version of this article (doi:10.1186/s12863-017-0500-z) contains supplementary material, which is available to authorized users.

No MeSH data available.