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Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China

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ABSTRACT

Background: Hirame rhabdovirus virus (HIRRV) is a rhabdovirus that causes acute hemorrhage disease in fish culture, resulting in a great economic loss in parts of Asia and Europe.

Methods: In this study, we isolated a virus strain named as CNPo2015 from cultured Japanese flounder in Shandong province, China. Cell isolation, electron microscopic observation, RT-PCR detection and phylogenetic analysis were used for virus identification. Further, artificial infection experiment was conducted for virulence testing.

Results: The gross signs included abdominal distension, fin reddening and yellow ascitic fluid in the abdominal cavity. Histopathological examination revealed marked cell degeneration and necrosis in the kidney. The tissue homogenates induced obvious cytopathic effects in EPC, FHM and FG cell lines. Electron microscopic observation showed the virus had a bullet-like shape with a capsule membrane. RT-PCR and sequencing analysis revealed that CNPo2015 belonged to the HIRRV with high sequence identity to HIRRV isolates. Infection experiment confirmed that the HIRRV CNPo2015 strain was virulent to flounder juveniles with a LD50 value of 1.0 × 105.9 TCID50/fish.

Conclusion: In conclusion, we described the first isolation and characterization of a HIRRV from Japanese flounder in China. This will provide a candidate material for further research on the infection mechanism and preventive strategies of HIRRV.

No MeSH data available.


Cumulative mortality rates of flounder juveniles after challenge with different doses of the HIRRV CNPo2015 through day 14 post-infection. Each group contained 30 fish
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Fig6: Cumulative mortality rates of flounder juveniles after challenge with different doses of the HIRRV CNPo2015 through day 14 post-infection. Each group contained 30 fish

Mentions: Flounder juveniles were inoculated with different doses of the HIRRV CNPo2015 strain. Symptoms such as abdominal distension and fin reddening appeared on day 4 and began to die on day 5 post-infection. At 14 days post-infection, fish infected with 107.5, 106.5, 105.5, 104.5 TCID50 of CNPo2015 strain had cumulative mortalities of 100, 60, 40 and 10%, respectively (Fig. 6). Mortality rates in each group were plotted and the LD50 in juvenile flounder was determined to be 1.0 × 105.9 TCID50/fish. No signs or mortalities were observed in the control fish during the experiment.Fig. 6


Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China
Cumulative mortality rates of flounder juveniles after challenge with different doses of the HIRRV CNPo2015 through day 14 post-infection. Each group contained 30 fish
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5384145&req=5

Fig6: Cumulative mortality rates of flounder juveniles after challenge with different doses of the HIRRV CNPo2015 through day 14 post-infection. Each group contained 30 fish
Mentions: Flounder juveniles were inoculated with different doses of the HIRRV CNPo2015 strain. Symptoms such as abdominal distension and fin reddening appeared on day 4 and began to die on day 5 post-infection. At 14 days post-infection, fish infected with 107.5, 106.5, 105.5, 104.5 TCID50 of CNPo2015 strain had cumulative mortalities of 100, 60, 40 and 10%, respectively (Fig. 6). Mortality rates in each group were plotted and the LD50 in juvenile flounder was determined to be 1.0 × 105.9 TCID50/fish. No signs or mortalities were observed in the control fish during the experiment.Fig. 6

View Article: PubMed Central - PubMed

ABSTRACT

Background: Hirame rhabdovirus virus (HIRRV) is a rhabdovirus that causes acute hemorrhage disease in fish culture, resulting in a great economic loss in parts of Asia and Europe.

Methods: In this study, we isolated a virus strain named as CNPo2015 from cultured Japanese flounder in Shandong province, China. Cell isolation, electron microscopic observation, RT-PCR detection and phylogenetic analysis were used for virus identification. Further, artificial infection experiment was conducted for virulence testing.

Results: The gross signs included abdominal distension, fin reddening and yellow ascitic fluid in the abdominal cavity. Histopathological examination revealed marked cell degeneration and necrosis in the kidney. The tissue homogenates induced obvious cytopathic effects in EPC, FHM and FG cell lines. Electron microscopic observation showed the virus had a bullet-like shape with a capsule membrane. RT-PCR and sequencing analysis revealed that CNPo2015 belonged to the HIRRV with high sequence identity to HIRRV isolates. Infection experiment confirmed that the HIRRV CNPo2015 strain was virulent to flounder juveniles with a LD50 value of 1.0 × 105.9 TCID50/fish.

Conclusion: In conclusion, we described the first isolation and characterization of a HIRRV from Japanese flounder in China. This will provide a candidate material for further research on the infection mechanism and preventive strategies of HIRRV.

No MeSH data available.