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Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China

View Article: PubMed Central - PubMed

ABSTRACT

Background: Hirame rhabdovirus virus (HIRRV) is a rhabdovirus that causes acute hemorrhage disease in fish culture, resulting in a great economic loss in parts of Asia and Europe.

Methods: In this study, we isolated a virus strain named as CNPo2015 from cultured Japanese flounder in Shandong province, China. Cell isolation, electron microscopic observation, RT-PCR detection and phylogenetic analysis were used for virus identification. Further, artificial infection experiment was conducted for virulence testing.

Results: The gross signs included abdominal distension, fin reddening and yellow ascitic fluid in the abdominal cavity. Histopathological examination revealed marked cell degeneration and necrosis in the kidney. The tissue homogenates induced obvious cytopathic effects in EPC, FHM and FG cell lines. Electron microscopic observation showed the virus had a bullet-like shape with a capsule membrane. RT-PCR and sequencing analysis revealed that CNPo2015 belonged to the HIRRV with high sequence identity to HIRRV isolates. Infection experiment confirmed that the HIRRV CNPo2015 strain was virulent to flounder juveniles with a LD50 value of 1.0 × 105.9 TCID50/fish.

Conclusion: In conclusion, we described the first isolation and characterization of a HIRRV from Japanese flounder in China. This will provide a candidate material for further research on the infection mechanism and preventive strategies of HIRRV.

No MeSH data available.


Related in: MedlinePlus

Morphology of the virions under electron microscope. a A large amount of virions aggregated on the surface of the cell. b Virions were wrapped by vesicles in the cytoplasm. Scale bars = 200 nm
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Fig3: Morphology of the virions under electron microscope. a A large amount of virions aggregated on the surface of the cell. b Virions were wrapped by vesicles in the cytoplasm. Scale bars = 200 nm

Mentions: Transmission electron microscopy showed that abundant viral particles were aggregated on the surface of cells at 2 days post inoculation (Fig. 3a). Meanwhile, some particles were also found inside the cytoplasm with a vesicle encapsulated (Fig. 3b). The intact virion exhibited a bullet-shaped capsid enclosed with envelope. Moreover, the virion averaged approximately 160 nm in length and 80 nm in width.Fig. 3


Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China
Morphology of the virions under electron microscope. a A large amount of virions aggregated on the surface of the cell. b Virions were wrapped by vesicles in the cytoplasm. Scale bars = 200 nm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5384145&req=5

Fig3: Morphology of the virions under electron microscope. a A large amount of virions aggregated on the surface of the cell. b Virions were wrapped by vesicles in the cytoplasm. Scale bars = 200 nm
Mentions: Transmission electron microscopy showed that abundant viral particles were aggregated on the surface of cells at 2 days post inoculation (Fig. 3a). Meanwhile, some particles were also found inside the cytoplasm with a vesicle encapsulated (Fig. 3b). The intact virion exhibited a bullet-shaped capsid enclosed with envelope. Moreover, the virion averaged approximately 160 nm in length and 80 nm in width.Fig. 3

View Article: PubMed Central - PubMed

ABSTRACT

Background: Hirame rhabdovirus virus (HIRRV) is a rhabdovirus that causes acute hemorrhage disease in fish culture, resulting in a great economic loss in parts of Asia and Europe.

Methods: In this study, we isolated a virus strain named as CNPo2015 from cultured Japanese flounder in Shandong province, China. Cell isolation, electron microscopic observation, RT-PCR detection and phylogenetic analysis were used for virus identification. Further, artificial infection experiment was conducted for virulence testing.

Results: The gross signs included abdominal distension, fin reddening and yellow ascitic fluid in the abdominal cavity. Histopathological examination revealed marked cell degeneration and necrosis in the kidney. The tissue homogenates induced obvious cytopathic effects in EPC, FHM and FG cell lines. Electron microscopic observation showed the virus had a bullet-like shape with a capsule membrane. RT-PCR and sequencing analysis revealed that CNPo2015 belonged to the HIRRV with high sequence identity to HIRRV isolates. Infection experiment confirmed that the HIRRV CNPo2015 strain was virulent to flounder juveniles with a LD50 value of 1.0 × 105.9 TCID50/fish.

Conclusion: In conclusion, we described the first isolation and characterization of a HIRRV from Japanese flounder in China. This will provide a candidate material for further research on the infection mechanism and preventive strategies of HIRRV.

No MeSH data available.


Related in: MedlinePlus