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A novel colloidal gold immunochromatography assay strip for the diagnosis of schistosomiasis japonica in domestic animals

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ABSTRACT

Background: Schistosomiasis remains a major public health concern in China and an epidemiological survey has revealed that schistosome-infected bovines and goats are the main transmission sources for the disease. Therefore, development of a sensitive technique for the diagnosis of schistosomiasis in domestic animals is necessary.

Method: A novel colloidal gold immunochromatography assay (GICA) strip was developed for detecting Schistosoma japonicum in domestic animals. The colloidal gold was conjugated with recombinant streptococcal protein G (rSPG). As the test and control lines, the schistosome soluble egg antigen and rSPG, respectively, were blotted on nitrocellulose membrane.

Results: The lowest detectable serum dilution was 1∶640 for schistosome-infected buffaloes. The cross-reaction rate of GICA was 14.29% with Paramphistomum sp. in buffaloes, 16.67% with Haemonchus sp. in goats, and 33.33% with Orientobilharzia sp. in goats. These results were slightly lower and similar to those obtained through ELISA. Moreover, the strips for detecting S. japonicum in mice, rabbits, buffaloes, and goats showed high sensitivity (100.00%, 100.00%, 100.00%, and 100.00%, respectively) and specificity (100.00%, 100.00%, 94.23%, and 88.64%, respectively). And the sensitivity or specificity of the GICA strips did not present any significant differences after storage for 12 months at room temperature. When compared with ELISA, the GICA strips exhibited similar sensitivity and specificity in the diagnosis of schistosomiasis in mice, rabbits, buffaloes, and goats. Besides, only 5 μl of serum are required for the test and the detection can be completed within 5 min.

Conclusion: This study is the first to develop a GICA strip using gold–rSPG conjugate for the diagnosing of schistosomiasis in domestic animals, and preliminary results showed that the developed strip may be suitable for large-scale screening of schistosomiasis in endemic areas.

Electronic supplementary material: The online version of this article (doi:10.1186/s40249-017-0297-z) contains supplementary material, which is available to authorized users.

No MeSH data available.


Related in: MedlinePlus

a Schistosomiasis diagnosis in a mice, b rabbits, c buffaloes and d goats using ELISA
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Fig7: a Schistosomiasis diagnosis in a mice, b rabbits, c buffaloes and d goats using ELISA

Mentions: Furthermore, buffalo and goat serum samples were employed to compare the sensitivity and specificity of GICA with those of ELISA in detecting S. japonicum. The serum samples from buffaloes and goats were determined as positive by fecal miracidium hatching test. The sensitivity of both the GICA strip and ELISA was 100% (80/80, 95% CI: 95.49%–100.00%) for the positive samples from buffaloes, whereas the specificity of the GICA strip was higher (94.23%, 49/52, 95% CI: 84.05%–98.79%) for the samples from uninfected buffaloes, when compared with that of ELISA (84.62%, 44/52, 95% CI: 71.92%–93.12%). Nevertheless, there was no significant difference between GICA and ELISA with regard to schistosomiasis diagnosis using buffalo serum (χ2 = 0.148, P > 0.05). Similarly, the sensitivity of both GICA and ELISA was 100% (73/73, 95% CI: 95.07%–100.00%) for the positive goat serum samples, whereas the specificity of GICA was higher (88.64%, 39/44, 95% CI: 75.44%–96.21%) for the samples from uninfected goats, when compared with that of ELISA (75.0%, 33/44, 95% CI: 59.66%–86.81%) (Table 3 and Fig. 7). However, no significant difference was noted between GICA and ELISA with respect to schistosomiasis diagnosis using goat serum (χ2 = 0.415, P > 0.05).Fig. 7


A novel colloidal gold immunochromatography assay strip for the diagnosis of schistosomiasis japonica in domestic animals
a Schistosomiasis diagnosis in a mice, b rabbits, c buffaloes and d goats using ELISA
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5384140&req=5

Fig7: a Schistosomiasis diagnosis in a mice, b rabbits, c buffaloes and d goats using ELISA
Mentions: Furthermore, buffalo and goat serum samples were employed to compare the sensitivity and specificity of GICA with those of ELISA in detecting S. japonicum. The serum samples from buffaloes and goats were determined as positive by fecal miracidium hatching test. The sensitivity of both the GICA strip and ELISA was 100% (80/80, 95% CI: 95.49%–100.00%) for the positive samples from buffaloes, whereas the specificity of the GICA strip was higher (94.23%, 49/52, 95% CI: 84.05%–98.79%) for the samples from uninfected buffaloes, when compared with that of ELISA (84.62%, 44/52, 95% CI: 71.92%–93.12%). Nevertheless, there was no significant difference between GICA and ELISA with regard to schistosomiasis diagnosis using buffalo serum (χ2 = 0.148, P > 0.05). Similarly, the sensitivity of both GICA and ELISA was 100% (73/73, 95% CI: 95.07%–100.00%) for the positive goat serum samples, whereas the specificity of GICA was higher (88.64%, 39/44, 95% CI: 75.44%–96.21%) for the samples from uninfected goats, when compared with that of ELISA (75.0%, 33/44, 95% CI: 59.66%–86.81%) (Table 3 and Fig. 7). However, no significant difference was noted between GICA and ELISA with respect to schistosomiasis diagnosis using goat serum (χ2 = 0.415, P > 0.05).Fig. 7

View Article: PubMed Central - PubMed

ABSTRACT

Background: Schistosomiasis remains a major public health concern in China and an epidemiological survey has revealed that schistosome-infected bovines and goats are the main transmission sources for the disease. Therefore, development of a sensitive technique for the diagnosis of schistosomiasis in domestic animals is necessary.

Method: A novel colloidal gold immunochromatography assay (GICA) strip was developed for detecting Schistosoma japonicum in domestic animals. The colloidal gold was conjugated with recombinant streptococcal protein G (rSPG). As the test and control lines, the schistosome soluble egg antigen and rSPG, respectively, were blotted on nitrocellulose membrane.

Results: The lowest detectable serum dilution was 1∶640 for schistosome-infected buffaloes. The cross-reaction rate of GICA was 14.29% with Paramphistomum sp. in buffaloes, 16.67% with Haemonchus sp. in goats, and 33.33% with Orientobilharzia sp. in goats. These results were slightly lower and similar to those obtained through ELISA. Moreover, the strips for detecting S. japonicum in mice, rabbits, buffaloes, and goats showed high sensitivity (100.00%, 100.00%, 100.00%, and 100.00%, respectively) and specificity (100.00%, 100.00%, 94.23%, and 88.64%, respectively). And the sensitivity or specificity of the GICA strips did not present any significant differences after storage for 12 months at room temperature. When compared with ELISA, the GICA strips exhibited similar sensitivity and specificity in the diagnosis of schistosomiasis in mice, rabbits, buffaloes, and goats. Besides, only 5 μl of serum are required for the test and the detection can be completed within 5 min.

Conclusion: This study is the first to develop a GICA strip using gold–rSPG conjugate for the diagnosing of schistosomiasis in domestic animals, and preliminary results showed that the developed strip may be suitable for large-scale screening of schistosomiasis in endemic areas.

Electronic supplementary material: The online version of this article (doi:10.1186/s40249-017-0297-z) contains supplementary material, which is available to authorized users.

No MeSH data available.


Related in: MedlinePlus