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Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses

View Article: PubMed Central - PubMed

ABSTRACT

Objectives:: To compare biofilm formations of two Staphylococcus epidermidis (S. epidermidis) isolates with known biofilm formation capacities on four different intraocular lenses (IOL) that have not been studied before.

Materials and methods:: Two isolates obtained from ocular surfaces and identified in previous studies and stored at -86 °C in 15% glycerol in the microbiology laboratory of the Anadolu University Department of Biology were purified and used in the study. The isolates were S. epidermidis KA 15.8 (ICA+), a known biofilm producer isolate positive for icaA, icaD and bap genes, and S. epidermidis KA 14.5 (ICA-), known as a non-biofilm producer isolate negative for icaA, icaD and bap genes. The biofilm formation capacities of the 2 isolates on 4 different IOLs were compared. Two of the IOLs were acrylic (UD613 [IOL A], Turkey; SA60AT [IOL B], USA), and the other two were polymethyl methacrylate (PMMA) (B60130C [IOL C], India; B55125C [IOL D], India). Bacterial enumeration and optical density measurements were done from biofilms that formed on the IOLs. Biofilms were imaged using scanning electron microscopy.

Results:: Mean bacterial counts on the IOLs were 7.1±0.4 log10 CFU/mL with the ICA+ isolate, and 6.7±0.8 log10 CFU/mL with the ICA- isolate; there were no statistically significant differences. Biofilm formation was lower with acrylic lenses than PMMA lenses with both isolates (p=0.009 and p=0.013). The highest biofilm production was obtained on IOL C (PMMA) (p<0.001) and the lowest was obtained on IOL A (hydrophilic acrylic) (p<0.001).

Conclusion:: Bacterial counts after biofilm formation were lower on acrylic lenses, especially hydrophilic acrylic with hydrophobic properties. Further animal and in vivo studies are required to support the findings of this study.

No MeSH data available.


Bacterial counts in the biofilms produced on the intraocular lensesIOL: Intraocular lens
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f3: Bacterial counts in the biofilms produced on the intraocular lensesIOL: Intraocular lens

Mentions: Enumeration of bacterial colonies revealed high counts for both strains on all the lenses (Figure 3). Bacterial counts on acrylic lenses were lower compared with the other IOLs. Bacterial adhesion was observed via electron microscopy (Figure 4). In the SEM images of the biofilms produced by ICA+ S. epidermidis, a multi-layer structure was evident with all of the lenses. In contrast, in images from the ICA- strain, this multi-layer structure did not appear on acrylic IOLs, but was evident on PMMA IOLs.


Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses
Bacterial counts in the biofilms produced on the intraocular lensesIOL: Intraocular lens
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384122&req=5

f3: Bacterial counts in the biofilms produced on the intraocular lensesIOL: Intraocular lens
Mentions: Enumeration of bacterial colonies revealed high counts for both strains on all the lenses (Figure 3). Bacterial counts on acrylic lenses were lower compared with the other IOLs. Bacterial adhesion was observed via electron microscopy (Figure 4). In the SEM images of the biofilms produced by ICA+ S. epidermidis, a multi-layer structure was evident with all of the lenses. In contrast, in images from the ICA- strain, this multi-layer structure did not appear on acrylic IOLs, but was evident on PMMA IOLs.

View Article: PubMed Central - PubMed

ABSTRACT

Objectives:: To compare biofilm formations of two Staphylococcus epidermidis (S. epidermidis) isolates with known biofilm formation capacities on four different intraocular lenses (IOL) that have not been studied before.

Materials and methods:: Two isolates obtained from ocular surfaces and identified in previous studies and stored at -86 °C in 15% glycerol in the microbiology laboratory of the Anadolu University Department of Biology were purified and used in the study. The isolates were S. epidermidis KA 15.8 (ICA+), a known biofilm producer isolate positive for icaA, icaD and bap genes, and S. epidermidis KA 14.5 (ICA-), known as a non-biofilm producer isolate negative for icaA, icaD and bap genes. The biofilm formation capacities of the 2 isolates on 4 different IOLs were compared. Two of the IOLs were acrylic (UD613 [IOL A], Turkey; SA60AT [IOL B], USA), and the other two were polymethyl methacrylate (PMMA) (B60130C [IOL C], India; B55125C [IOL D], India). Bacterial enumeration and optical density measurements were done from biofilms that formed on the IOLs. Biofilms were imaged using scanning electron microscopy.

Results:: Mean bacterial counts on the IOLs were 7.1±0.4 log10 CFU/mL with the ICA+ isolate, and 6.7±0.8 log10 CFU/mL with the ICA- isolate; there were no statistically significant differences. Biofilm formation was lower with acrylic lenses than PMMA lenses with both isolates (p=0.009 and p=0.013). The highest biofilm production was obtained on IOL C (PMMA) (p<0.001) and the lowest was obtained on IOL A (hydrophilic acrylic) (p<0.001).

Conclusion:: Bacterial counts after biofilm formation were lower on acrylic lenses, especially hydrophilic acrylic with hydrophobic properties. Further animal and in vivo studies are required to support the findings of this study.

No MeSH data available.