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Sensory genes identification with head transcriptome of the migratory armyworm, Mythimna separata

View Article: PubMed Central - PubMed

ABSTRACT

Sensory system plays important roles in a wide array of insect’s behavior and physiological events, including the host landing and locating, feeding, flying, sex responding, mating and oviposition which happen independently and in sequence. The armyworm Mythimna separata (Lepidoptera: Noctuidae) of migratory insect is destructive for alimentarn crop and economic crop throughout the world. Here we present the high throughput sequencing of the head transcriptome and identify members of the major sensory genes which are crucial for armyworm’s success worldwide, including 8 opsins, 22 chemosensory proteins, 50 odorant binding proteins, 60 odorant receptors, 8 gustatory receptors, 24 ionotropic receptors, and 2 sensory neuron membrane proteins. It is worth noting that a duplication of the LW opsin gene exists in this insect. Several genes were clustered with functionally validated genes, such as Co-receptors of OR and IR, PBPs, PRs, CO2 GRs, bitter GRs and sweet GRs, were also identified. The transcriptome gene library provided the basis for further studies that elucidate the fundamental molecular mechanism of biology and control in M. separata. Our research exhibits the first comprehensive catalogue of the sensory genes fundamental for success and distribution in M. separata, which are potential novel targets for pest control strategies.

No MeSH data available.


Phylogenetic tree of gustatory receptors (GRs).GRs from Lepidopteran, Coleopteran, Dipteran. The specific clades are marked. Node support was assessed with 1000 bootstrap replicates.
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f5: Phylogenetic tree of gustatory receptors (GRs).GRs from Lepidopteran, Coleopteran, Dipteran. The specific clades are marked. Node support was assessed with 1000 bootstrap replicates.

Mentions: Eight candidate gustatory receptors (GRs) transcripts with very low expression levels (Transcript abundance levels ranged from 0.46 to 1.94 FPKM) were identified (Supplementary Data S3). Only one candidate M. separata GRs represent full-length protein and most of them represent partial length fragments. They encoded overlapping but distinct sequences, which established the proteins as components of independent genes. As expected with other insect GRs, transmembrane domain and topology predictions indicated that full-length transcripts most likely possess between zero and two domains with an intracellular N-terminus and extracellular C-terminus (Supplementary Data S5). A phylogenetic tree was constructed using M. separata GRs (8), B. mori GRs (33), P. xylostella GRs (14), D. plexippus GRs (3), H. armigera GRs (9), H. assulta GRs (18), M. sexta GRs (45), T. castaneum GRs (57) and D. melanogaster GRs (27) (Fig. 5). All candidate M. separata GRs cluster with CO2, sugar, and putative bitter receptor families.


Sensory genes identification with head transcriptome of the migratory armyworm, Mythimna separata
Phylogenetic tree of gustatory receptors (GRs).GRs from Lepidopteran, Coleopteran, Dipteran. The specific clades are marked. Node support was assessed with 1000 bootstrap replicates.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384095&req=5

f5: Phylogenetic tree of gustatory receptors (GRs).GRs from Lepidopteran, Coleopteran, Dipteran. The specific clades are marked. Node support was assessed with 1000 bootstrap replicates.
Mentions: Eight candidate gustatory receptors (GRs) transcripts with very low expression levels (Transcript abundance levels ranged from 0.46 to 1.94 FPKM) were identified (Supplementary Data S3). Only one candidate M. separata GRs represent full-length protein and most of them represent partial length fragments. They encoded overlapping but distinct sequences, which established the proteins as components of independent genes. As expected with other insect GRs, transmembrane domain and topology predictions indicated that full-length transcripts most likely possess between zero and two domains with an intracellular N-terminus and extracellular C-terminus (Supplementary Data S5). A phylogenetic tree was constructed using M. separata GRs (8), B. mori GRs (33), P. xylostella GRs (14), D. plexippus GRs (3), H. armigera GRs (9), H. assulta GRs (18), M. sexta GRs (45), T. castaneum GRs (57) and D. melanogaster GRs (27) (Fig. 5). All candidate M. separata GRs cluster with CO2, sugar, and putative bitter receptor families.

View Article: PubMed Central - PubMed

ABSTRACT

Sensory system plays important roles in a wide array of insect’s behavior and physiological events, including the host landing and locating, feeding, flying, sex responding, mating and oviposition which happen independently and in sequence. The armyworm Mythimna separata (Lepidoptera: Noctuidae) of migratory insect is destructive for alimentarn crop and economic crop throughout the world. Here we present the high throughput sequencing of the head transcriptome and identify members of the major sensory genes which are crucial for armyworm’s success worldwide, including 8 opsins, 22 chemosensory proteins, 50 odorant binding proteins, 60 odorant receptors, 8 gustatory receptors, 24 ionotropic receptors, and 2 sensory neuron membrane proteins. It is worth noting that a duplication of the LW opsin gene exists in this insect. Several genes were clustered with functionally validated genes, such as Co-receptors of OR and IR, PBPs, PRs, CO2 GRs, bitter GRs and sweet GRs, were also identified. The transcriptome gene library provided the basis for further studies that elucidate the fundamental molecular mechanism of biology and control in M. separata. Our research exhibits the first comprehensive catalogue of the sensory genes fundamental for success and distribution in M. separata, which are potential novel targets for pest control strategies.

No MeSH data available.