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In-vivo Comparison of 18 F-FLT uptake, CT Number, Tumor Volume in Evaluation of Repopulation during Radiotherapy for Lung cancer

View Article: PubMed Central - PubMed

ABSTRACT

Accelerated repopulation has been observed in various tumors. This study was aimed to evaluate the potential of 3′-deoxy-3′-18F-fluorothymidine (18F-FLT) uptake and Computed Tomography Number (CTN) in monitoring tumor responses to radiotherapy compared with tumor volume (TV) changes. Tumor bearing nude mice were assigned to either irradiated daily or every second day group and then randomized to 6 sub-groups to receive 0Gy, 6Gy, 12Gy, 18Gy, 24Gy, 36Gy irradiation, respectively. TV was measured every 3 days. 18F-FLT micro-PET/CT scans were performed after irradiation being completed. Tumor sections were stained to calculate the immunohistochemical (Ki-67) labeling index (LI). Comparison analysis between FLT uptake parameters, CTNs, VTs and Ki-67 LI results were conducted to determine the correlation. Ki-67 LI increased significantly after 6 times of irradiation at irradiated daily group and after 3 times at irradiated every second day group, suggesting accelerated repopulation. No shrinkage of TV was noticed at two groups during irradiation delivery. Both 18F-FLT uptake and CTN increased significantly after irradiation of 12Gy/6f/6d and 6Gy/3f/6d. Comparison analysis found a significant relationship between Ki-67 LI and 18F-FLT uptake parameters as well as CTN. Both 18F-FLT PET and CT have the potential to reflect the tumor proliferative response during radiation delivery.

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Related in: MedlinePlus

Dose-response curves for FLT uptake parameters, CTN changes, Ki67 LI in irradiated daily and every second group.In order to balance the four curves in the same figure, the ratio of CTN/10 was used to replace CTN (in HU) and the KI67 LI was expressed in decimal point format. (A) In irradiated daily group, both FLT uptake (SUVmax, SUVmean, T/NT ratio) and CTN increases were observed after 6 times of irradiation. (B) In irradiated every second day group, FLT uptake and CTN increased significantly after irradiation of 6Gy/3f compared with non-irradiated tumors.
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f2: Dose-response curves for FLT uptake parameters, CTN changes, Ki67 LI in irradiated daily and every second group.In order to balance the four curves in the same figure, the ratio of CTN/10 was used to replace CTN (in HU) and the KI67 LI was expressed in decimal point format. (A) In irradiated daily group, both FLT uptake (SUVmax, SUVmean, T/NT ratio) and CTN increases were observed after 6 times of irradiation. (B) In irradiated every second day group, FLT uptake and CTN increased significantly after irradiation of 6Gy/3f compared with non-irradiated tumors.

Mentions: Figure 2 showed the changes of Ki-67 LI among different treatment groups. The mean Ki-67 LI for non-irradiated tumors was 79.01% and 76.54%. A significant increase to 81.98% and 78.30% was observed after daily irradiation of 6 fractions in 6 days (12Gy/6f/6d) (P < 0.0001) and after every second day irradiation of 3 fractions in 6 days (6Gy/3f/6d) (P = 0.002), respectively. Taken together, these Ki-67 LI changes under different fractionation schemes validated the A549 xenograft model of accelerated tumor repopulation.


In-vivo Comparison of 18 F-FLT uptake, CT Number, Tumor Volume in Evaluation of Repopulation during Radiotherapy for Lung cancer
Dose-response curves for FLT uptake parameters, CTN changes, Ki67 LI in irradiated daily and every second group.In order to balance the four curves in the same figure, the ratio of CTN/10 was used to replace CTN (in HU) and the KI67 LI was expressed in decimal point format. (A) In irradiated daily group, both FLT uptake (SUVmax, SUVmean, T/NT ratio) and CTN increases were observed after 6 times of irradiation. (B) In irradiated every second day group, FLT uptake and CTN increased significantly after irradiation of 6Gy/3f compared with non-irradiated tumors.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384084&req=5

f2: Dose-response curves for FLT uptake parameters, CTN changes, Ki67 LI in irradiated daily and every second group.In order to balance the four curves in the same figure, the ratio of CTN/10 was used to replace CTN (in HU) and the KI67 LI was expressed in decimal point format. (A) In irradiated daily group, both FLT uptake (SUVmax, SUVmean, T/NT ratio) and CTN increases were observed after 6 times of irradiation. (B) In irradiated every second day group, FLT uptake and CTN increased significantly after irradiation of 6Gy/3f compared with non-irradiated tumors.
Mentions: Figure 2 showed the changes of Ki-67 LI among different treatment groups. The mean Ki-67 LI for non-irradiated tumors was 79.01% and 76.54%. A significant increase to 81.98% and 78.30% was observed after daily irradiation of 6 fractions in 6 days (12Gy/6f/6d) (P < 0.0001) and after every second day irradiation of 3 fractions in 6 days (6Gy/3f/6d) (P = 0.002), respectively. Taken together, these Ki-67 LI changes under different fractionation schemes validated the A549 xenograft model of accelerated tumor repopulation.

View Article: PubMed Central - PubMed

ABSTRACT

Accelerated repopulation has been observed in various tumors. This study was aimed to evaluate the potential of 3&prime;-deoxy-3&prime;-18F-fluorothymidine (18F-FLT) uptake and Computed Tomography Number (CTN) in monitoring tumor responses to radiotherapy compared with tumor volume (TV) changes. Tumor bearing nude mice were assigned to either irradiated daily or every second day group and then randomized to 6 sub-groups to receive 0Gy, 6Gy, 12Gy, 18Gy, 24Gy, 36Gy irradiation, respectively. TV was measured every 3 days. 18F-FLT micro-PET/CT scans were performed after irradiation being completed. Tumor sections were stained to calculate the immunohistochemical (Ki-67) labeling index (LI). Comparison analysis between FLT uptake parameters, CTNs, VTs and Ki-67 LI results were conducted to determine the correlation. Ki-67 LI increased significantly after 6 times of irradiation at irradiated daily group and after 3 times at irradiated every second day group, suggesting accelerated repopulation. No shrinkage of TV was noticed at two groups during irradiation delivery. Both 18F-FLT uptake and CTN increased significantly after irradiation of 12Gy/6f/6d and 6Gy/3f/6d. Comparison analysis found a significant relationship between Ki-67 LI and 18F-FLT uptake parameters as well as CTN. Both 18F-FLT PET and CT have the potential to reflect the tumor proliferative response during radiation delivery.

No MeSH data available.


Related in: MedlinePlus