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In-vivo Comparison of 18 F-FLT uptake, CT Number, Tumor Volume in Evaluation of Repopulation during Radiotherapy for Lung cancer

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ABSTRACT

Accelerated repopulation has been observed in various tumors. This study was aimed to evaluate the potential of 3′-deoxy-3′-18F-fluorothymidine (18F-FLT) uptake and Computed Tomography Number (CTN) in monitoring tumor responses to radiotherapy compared with tumor volume (TV) changes. Tumor bearing nude mice were assigned to either irradiated daily or every second day group and then randomized to 6 sub-groups to receive 0Gy, 6Gy, 12Gy, 18Gy, 24Gy, 36Gy irradiation, respectively. TV was measured every 3 days. 18F-FLT micro-PET/CT scans were performed after irradiation being completed. Tumor sections were stained to calculate the immunohistochemical (Ki-67) labeling index (LI). Comparison analysis between FLT uptake parameters, CTNs, VTs and Ki-67 LI results were conducted to determine the correlation. Ki-67 LI increased significantly after 6 times of irradiation at irradiated daily group and after 3 times at irradiated every second day group, suggesting accelerated repopulation. No shrinkage of TV was noticed at two groups during irradiation delivery. Both 18F-FLT uptake and CTN increased significantly after irradiation of 12Gy/6f/6d and 6Gy/3f/6d. Comparison analysis found a significant relationship between Ki-67 LI and 18F-FLT uptake parameters as well as CTN. Both 18F-FLT PET and CT have the potential to reflect the tumor proliferative response during radiation delivery.

No MeSH data available.


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Whole body PET and CT imaging of the No. 2 mice in 3f/6d group.(A) 18F-FLT PET imaging (B) CT imaging. Arrows point to tumor.
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f1: Whole body PET and CT imaging of the No. 2 mice in 3f/6d group.(A) 18F-FLT PET imaging (B) CT imaging. Arrows point to tumor.

Mentions: All of the 120 mice (60 for each fractionation group) received the given irradiation, 18F-FLT micro-PET scans and micro-CT scans as planned. Figure 1 showed one example of the whole body PET and CT imaging of the No 2 mouse in 3f/6d group. The tumor lesion had a good uptake of 18F-FLT, while the normal tissue maintained a low level of uptake of the imaging probe. The high target-to-background ratios made the PET imaging contrasting and valuable (Fig. 1A). A faint outline of tumor could be observed in the CT imaging (Fig. 1B). Tumor volumes were evaluated every 3 days.


In-vivo Comparison of 18 F-FLT uptake, CT Number, Tumor Volume in Evaluation of Repopulation during Radiotherapy for Lung cancer
Whole body PET and CT imaging of the No. 2 mice in 3f/6d group.(A) 18F-FLT PET imaging (B) CT imaging. Arrows point to tumor.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384084&req=5

f1: Whole body PET and CT imaging of the No. 2 mice in 3f/6d group.(A) 18F-FLT PET imaging (B) CT imaging. Arrows point to tumor.
Mentions: All of the 120 mice (60 for each fractionation group) received the given irradiation, 18F-FLT micro-PET scans and micro-CT scans as planned. Figure 1 showed one example of the whole body PET and CT imaging of the No 2 mouse in 3f/6d group. The tumor lesion had a good uptake of 18F-FLT, while the normal tissue maintained a low level of uptake of the imaging probe. The high target-to-background ratios made the PET imaging contrasting and valuable (Fig. 1A). A faint outline of tumor could be observed in the CT imaging (Fig. 1B). Tumor volumes were evaluated every 3 days.

View Article: PubMed Central - PubMed

ABSTRACT

Accelerated repopulation has been observed in various tumors. This study was aimed to evaluate the potential of 3′-deoxy-3′-18F-fluorothymidine (18F-FLT) uptake and Computed Tomography Number (CTN) in monitoring tumor responses to radiotherapy compared with tumor volume (TV) changes. Tumor bearing nude mice were assigned to either irradiated daily or every second day group and then randomized to 6 sub-groups to receive 0Gy, 6Gy, 12Gy, 18Gy, 24Gy, 36Gy irradiation, respectively. TV was measured every 3 days. 18F-FLT micro-PET/CT scans were performed after irradiation being completed. Tumor sections were stained to calculate the immunohistochemical (Ki-67) labeling index (LI). Comparison analysis between FLT uptake parameters, CTNs, VTs and Ki-67 LI results were conducted to determine the correlation. Ki-67 LI increased significantly after 6 times of irradiation at irradiated daily group and after 3 times at irradiated every second day group, suggesting accelerated repopulation. No shrinkage of TV was noticed at two groups during irradiation delivery. Both 18F-FLT uptake and CTN increased significantly after irradiation of 12Gy/6f/6d and 6Gy/3f/6d. Comparison analysis found a significant relationship between Ki-67 LI and 18F-FLT uptake parameters as well as CTN. Both 18F-FLT PET and CT have the potential to reflect the tumor proliferative response during radiation delivery.

No MeSH data available.


Related in: MedlinePlus