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Thymoquinone Attenuates Brain Injury via an Anti-oxidative Pathway in a Status Epilepticus Rat Model

View Article: PubMed Central - PubMed

ABSTRACT

Aim: Status epilepticus (SE) results in the generation of reactive oxygen species (ROS), which contribute to seizure-induced brain injury. It is well known that oxidative stress plays a pivotal role in status epilepticus (SE). Thymoquinone (TQ) is a bioactive monomer extracted from black cumin (Nigella sativa) seed oil that has anti-inflammatory, anti-cancer, and antioxidant activity in various diseases. This study evaluated the protective effects of TQ on brain injury in a lithium-pilocarpine rat model of SE and investigated the underlying mechanism related to antioxidative pathway.

Methods: Electroencephalogram and Racine scale were used to value seizure severity. Passive-avoidance test was used to determine learning and memory function. Moreover, anti-oxidative activity of TQ was observed using Western blot and super oxide dismutase (SOD) activity assay.

Results: Latency to SE increased in the TQ-pretreated group compared with rats in the model group, while the total power was significantly lower. Seizure severity measured on the Racine scale was significantly lower in the TQ group compared with the model group. Results of behavioral experiments suggest that TQ may also have a protective effect on learning and memory function. Investigation of the protective mechanism of TQ showed that TQ-pretreatment significantly increased the expression of Nrf2, HO-1 proteins and SOD in the hippocampus.

Conclusion: These findings showed that TQ attenuated brain injury induced by SE via an anti-oxidative pathway.

No MeSH data available.


Related in: MedlinePlus

Effect of TQ on the epileptic behavior of SE rats. Latent period of the step-through test. *model group vs. control group, p<0.05 Δ TQ group vs. model group, p<0.05.
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j_tnsci-2017-0003_fig_003: Effect of TQ on the epileptic behavior of SE rats. Latent period of the step-through test. *model group vs. control group, p<0.05 Δ TQ group vs. model group, p<0.05.

Mentions: The step-through test was used to estimate the effect of TQ on cognitive function. As shown in Figure 3, post-SE latent period in model group rats was significantly shorter than that observed pre-SE, thus indicating that SE impaired learning and memory. Compared with the rats in model group, the post-SE escape latency in TQ group rats was significantly longer (p <0.05). These results indicated the improvement of spatial learning and memory in TQ group.


Thymoquinone Attenuates Brain Injury via an Anti-oxidative Pathway in a Status Epilepticus Rat Model
Effect of TQ on the epileptic behavior of SE rats. Latent period of the step-through test. *model group vs. control group, p<0.05 Δ TQ group vs. model group, p<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384046&req=5

j_tnsci-2017-0003_fig_003: Effect of TQ on the epileptic behavior of SE rats. Latent period of the step-through test. *model group vs. control group, p<0.05 Δ TQ group vs. model group, p<0.05.
Mentions: The step-through test was used to estimate the effect of TQ on cognitive function. As shown in Figure 3, post-SE latent period in model group rats was significantly shorter than that observed pre-SE, thus indicating that SE impaired learning and memory. Compared with the rats in model group, the post-SE escape latency in TQ group rats was significantly longer (p <0.05). These results indicated the improvement of spatial learning and memory in TQ group.

View Article: PubMed Central - PubMed

ABSTRACT

Aim: Status epilepticus (SE) results in the generation of reactive oxygen species (ROS), which contribute to seizure-induced brain injury. It is well known that oxidative stress plays a pivotal role in status epilepticus (SE). Thymoquinone (TQ) is a bioactive monomer extracted from black cumin (Nigella sativa) seed oil that has anti-inflammatory, anti-cancer, and antioxidant activity in various diseases. This study evaluated the protective effects of TQ on brain injury in a lithium-pilocarpine rat model of SE and investigated the underlying mechanism related to antioxidative pathway.

Methods: Electroencephalogram and Racine scale were used to value seizure severity. Passive-avoidance test was used to determine learning and memory function. Moreover, anti-oxidative activity of TQ was observed using Western blot and super oxide dismutase (SOD) activity assay.

Results: Latency to SE increased in the TQ-pretreated group compared with rats in the model group, while the total power was significantly lower. Seizure severity measured on the Racine scale was significantly lower in the TQ group compared with the model group. Results of behavioral experiments suggest that TQ may also have a protective effect on learning and memory function. Investigation of the protective mechanism of TQ showed that TQ-pretreatment significantly increased the expression of Nrf2, HO-1 proteins and SOD in the hippocampus.

Conclusion: These findings showed that TQ attenuated brain injury induced by SE via an anti-oxidative pathway.

No MeSH data available.


Related in: MedlinePlus