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Methane protects against polyethylene glycol-induced osmotic stress in maize by improving sugar and ascorbic acid metabolism

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ABSTRACT

Although aerobic methane (CH4) release from plants leads to an intense scientific and public controversy in the recent years, the potential functions of endogenous CH4 production in plants are still largely unknown. Here, we reported that polyethylene glycol (PEG)-induced osmotic stress significantly increased CH4 production and soluble sugar contents in maize (Zea mays L.) root tissues. These enhancements were more pronounced in the drought stress-tolerant cultivar Zhengdan 958 (ZD958) than in the drought stress-sensitive cultivar Zhongjiangyu No.1 (ZJY1). Exogenously applied 0.65 mM CH4 not only increased endogenous CH4 production, but also decreased the contents of thiobarbituric acid reactive substances. PEG-induced water deficit symptoms, such as decreased biomass and relative water contents in both root and shoot tissues, were also alleviated. These beneficial responses paralleled the increases in the contents of soluble sugar and the reduced ascorbic acid (AsA), and the ratio of AsA/dehydroascorbate (DHA). Further comparison of transcript profiles of some key enzymes in sugar and AsA metabolism suggested that CH4 might participate in sugar signaling, which in turn increased AsA production and recycling. Together, these results suggested that CH4 might function as a gaseous molecule that enhances osmotic stress tolerance in maize by modulating sugar and AsA metabolism.

No MeSH data available.


Regulation of redox state by CH4.5-d-old maize seedlings of ZD958 and ZJY1 were preincubated in the solution containing 0.65 mM CH4 for 1 d, and then transferred to half-strength Hoagland solutions with or without 20% PEG-6000 for 2 d. Afterwards, the contents of endogenous H2O2 (a) in root tissues was analyzed. Meanwhile, the contents of reduced ascorbic acid (AsA; b), dehydroascorbate (DHA; c), and AsA/DHA ratio (d) in root tissues were determined according to the method described by Law et al.51. Control seedlings were incubated in Hoagland solution alone. Data are presented as means ± SE (5 root parts per experiment performed three times). Bars with different letters denote significant differences according to multiple comparisons (P < 0.05).
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f6: Regulation of redox state by CH4.5-d-old maize seedlings of ZD958 and ZJY1 were preincubated in the solution containing 0.65 mM CH4 for 1 d, and then transferred to half-strength Hoagland solutions with or without 20% PEG-6000 for 2 d. Afterwards, the contents of endogenous H2O2 (a) in root tissues was analyzed. Meanwhile, the contents of reduced ascorbic acid (AsA; b), dehydroascorbate (DHA; c), and AsA/DHA ratio (d) in root tissues were determined according to the method described by Law et al.51. Control seedlings were incubated in Hoagland solution alone. Data are presented as means ± SE (5 root parts per experiment performed three times). Bars with different letters denote significant differences according to multiple comparisons (P < 0.05).

Mentions: PEG stress-induced ROS has been demonstrated to cause oxidative damage to plants, and H2O2 and O2− are believed to be the most important components. The effect of CH4 on the PEG-induced ROS overproduction was further investigated. Our results showed that PEG induced significant increases in the levels of H2O2 and O2− in root tissues of both cultivars (Fig. 6a; Supplementary Fig. S3). CH4 pretreatment significantly reduced PEG-induced ROS production, with more effects observed in root tissues of ZD958 than in ZJY1.


Methane protects against polyethylene glycol-induced osmotic stress in maize by improving sugar and ascorbic acid metabolism
Regulation of redox state by CH4.5-d-old maize seedlings of ZD958 and ZJY1 were preincubated in the solution containing 0.65 mM CH4 for 1 d, and then transferred to half-strength Hoagland solutions with or without 20% PEG-6000 for 2 d. Afterwards, the contents of endogenous H2O2 (a) in root tissues was analyzed. Meanwhile, the contents of reduced ascorbic acid (AsA; b), dehydroascorbate (DHA; c), and AsA/DHA ratio (d) in root tissues were determined according to the method described by Law et al.51. Control seedlings were incubated in Hoagland solution alone. Data are presented as means ± SE (5 root parts per experiment performed three times). Bars with different letters denote significant differences according to multiple comparisons (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384014&req=5

f6: Regulation of redox state by CH4.5-d-old maize seedlings of ZD958 and ZJY1 were preincubated in the solution containing 0.65 mM CH4 for 1 d, and then transferred to half-strength Hoagland solutions with or without 20% PEG-6000 for 2 d. Afterwards, the contents of endogenous H2O2 (a) in root tissues was analyzed. Meanwhile, the contents of reduced ascorbic acid (AsA; b), dehydroascorbate (DHA; c), and AsA/DHA ratio (d) in root tissues were determined according to the method described by Law et al.51. Control seedlings were incubated in Hoagland solution alone. Data are presented as means ± SE (5 root parts per experiment performed three times). Bars with different letters denote significant differences according to multiple comparisons (P < 0.05).
Mentions: PEG stress-induced ROS has been demonstrated to cause oxidative damage to plants, and H2O2 and O2− are believed to be the most important components. The effect of CH4 on the PEG-induced ROS overproduction was further investigated. Our results showed that PEG induced significant increases in the levels of H2O2 and O2− in root tissues of both cultivars (Fig. 6a; Supplementary Fig. S3). CH4 pretreatment significantly reduced PEG-induced ROS production, with more effects observed in root tissues of ZD958 than in ZJY1.

View Article: PubMed Central - PubMed

ABSTRACT

Although aerobic methane (CH4) release from plants leads to an intense scientific and public controversy in the recent years, the potential functions of endogenous CH4 production in plants are still largely unknown. Here, we reported that polyethylene glycol (PEG)-induced osmotic stress significantly increased CH4 production and soluble sugar contents in maize (Zea mays L.) root tissues. These enhancements were more pronounced in the drought stress-tolerant cultivar Zhengdan 958 (ZD958) than in the drought stress-sensitive cultivar Zhongjiangyu No.1 (ZJY1). Exogenously applied 0.65&thinsp;mM CH4 not only increased endogenous CH4 production, but also decreased the contents of thiobarbituric acid reactive substances. PEG-induced water deficit symptoms, such as decreased biomass and relative water contents in both root and shoot tissues, were also alleviated. These beneficial responses paralleled the increases in the contents of soluble sugar and the reduced ascorbic acid (AsA), and the ratio of AsA/dehydroascorbate (DHA). Further comparison of transcript profiles of some key enzymes in sugar and AsA metabolism suggested that CH4 might participate in sugar signaling, which in turn increased AsA production and recycling. Together, these results suggested that CH4 might function as a gaseous molecule that enhances osmotic stress tolerance in maize by modulating sugar and AsA metabolism.

No MeSH data available.