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Methane protects against polyethylene glycol-induced osmotic stress in maize by improving sugar and ascorbic acid metabolism

View Article: PubMed Central - PubMed

ABSTRACT

Although aerobic methane (CH4) release from plants leads to an intense scientific and public controversy in the recent years, the potential functions of endogenous CH4 production in plants are still largely unknown. Here, we reported that polyethylene glycol (PEG)-induced osmotic stress significantly increased CH4 production and soluble sugar contents in maize (Zea mays L.) root tissues. These enhancements were more pronounced in the drought stress-tolerant cultivar Zhengdan 958 (ZD958) than in the drought stress-sensitive cultivar Zhongjiangyu No.1 (ZJY1). Exogenously applied 0.65 mM CH4 not only increased endogenous CH4 production, but also decreased the contents of thiobarbituric acid reactive substances. PEG-induced water deficit symptoms, such as decreased biomass and relative water contents in both root and shoot tissues, were also alleviated. These beneficial responses paralleled the increases in the contents of soluble sugar and the reduced ascorbic acid (AsA), and the ratio of AsA/dehydroascorbate (DHA). Further comparison of transcript profiles of some key enzymes in sugar and AsA metabolism suggested that CH4 might participate in sugar signaling, which in turn increased AsA production and recycling. Together, these results suggested that CH4 might function as a gaseous molecule that enhances osmotic stress tolerance in maize by modulating sugar and AsA metabolism.

No MeSH data available.


Phenotypic and physiological comparisons of two maize cultivars upon PEG stress.(a) 5-d-old seedlings of ZD958 and ZJY1 were transferred to half-strength Hoagland solutions containing the indicated concentrations of PEG-6000 (PEG) for another 5 d. Photographs were then taken. Bar = 10 cm. Meanwhile, fresh weight (b) and relative water content (RWC; c) were measured in both root and shoot tissues. Data are presented as means ± SE (5 root or shoot parts per experiment performed three times). Bars with different letters denote significant differences according to multiple comparisons (P < 0.05).
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f1: Phenotypic and physiological comparisons of two maize cultivars upon PEG stress.(a) 5-d-old seedlings of ZD958 and ZJY1 were transferred to half-strength Hoagland solutions containing the indicated concentrations of PEG-6000 (PEG) for another 5 d. Photographs were then taken. Bar = 10 cm. Meanwhile, fresh weight (b) and relative water content (RWC; c) were measured in both root and shoot tissues. Data are presented as means ± SE (5 root or shoot parts per experiment performed three times). Bars with different letters denote significant differences according to multiple comparisons (P < 0.05).

Mentions: In this study, two highly productive maize cultivars, namely the osmotic-stress tolerant Zhengdan 958 (ZD958) and the sensitive Zhongjiangyu No.1 (ZJY1), were selected for osmotic stress sensitivity analysis. 5-d-old seedlings were treated with different concentrations of PEG-6000 for another 5 d, and phenotypic changes of both root and shoot tissues were monitored. PEG-6000 treatment significantly inhibited maize seedling growth in a dose-dependent manner (Fig. 1a), and the growth inhibition was more pronounced in ZJY1 than that of ZD958. For example, compared to the PEG-free control samples, the fresh weight of root tissues in ZD958 with 15%, 20%, and 25% PEG-6000 treatments decreased to 86.5 ± 7.7%, 70.9 ± 6.5%, and 39.6 ± 10.2%, and with 53.4 ± 5.4%, 39.4 ± 5.6%, and 24.5 ± 4.2% in ZJY1 (Fig. 1b). A similar trend was observed for the shoot tissues of these two maize cultivars under different concentrations of PEG-6000.


Methane protects against polyethylene glycol-induced osmotic stress in maize by improving sugar and ascorbic acid metabolism
Phenotypic and physiological comparisons of two maize cultivars upon PEG stress.(a) 5-d-old seedlings of ZD958 and ZJY1 were transferred to half-strength Hoagland solutions containing the indicated concentrations of PEG-6000 (PEG) for another 5 d. Photographs were then taken. Bar = 10 cm. Meanwhile, fresh weight (b) and relative water content (RWC; c) were measured in both root and shoot tissues. Data are presented as means ± SE (5 root or shoot parts per experiment performed three times). Bars with different letters denote significant differences according to multiple comparisons (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384014&req=5

f1: Phenotypic and physiological comparisons of two maize cultivars upon PEG stress.(a) 5-d-old seedlings of ZD958 and ZJY1 were transferred to half-strength Hoagland solutions containing the indicated concentrations of PEG-6000 (PEG) for another 5 d. Photographs were then taken. Bar = 10 cm. Meanwhile, fresh weight (b) and relative water content (RWC; c) were measured in both root and shoot tissues. Data are presented as means ± SE (5 root or shoot parts per experiment performed three times). Bars with different letters denote significant differences according to multiple comparisons (P < 0.05).
Mentions: In this study, two highly productive maize cultivars, namely the osmotic-stress tolerant Zhengdan 958 (ZD958) and the sensitive Zhongjiangyu No.1 (ZJY1), were selected for osmotic stress sensitivity analysis. 5-d-old seedlings were treated with different concentrations of PEG-6000 for another 5 d, and phenotypic changes of both root and shoot tissues were monitored. PEG-6000 treatment significantly inhibited maize seedling growth in a dose-dependent manner (Fig. 1a), and the growth inhibition was more pronounced in ZJY1 than that of ZD958. For example, compared to the PEG-free control samples, the fresh weight of root tissues in ZD958 with 15%, 20%, and 25% PEG-6000 treatments decreased to 86.5 ± 7.7%, 70.9 ± 6.5%, and 39.6 ± 10.2%, and with 53.4 ± 5.4%, 39.4 ± 5.6%, and 24.5 ± 4.2% in ZJY1 (Fig. 1b). A similar trend was observed for the shoot tissues of these two maize cultivars under different concentrations of PEG-6000.

View Article: PubMed Central - PubMed

ABSTRACT

Although aerobic methane (CH4) release from plants leads to an intense scientific and public controversy in the recent years, the potential functions of endogenous CH4 production in plants are still largely unknown. Here, we reported that polyethylene glycol (PEG)-induced osmotic stress significantly increased CH4 production and soluble sugar contents in maize (Zea mays L.) root tissues. These enhancements were more pronounced in the drought stress-tolerant cultivar Zhengdan 958 (ZD958) than in the drought stress-sensitive cultivar Zhongjiangyu No.1 (ZJY1). Exogenously applied 0.65&thinsp;mM CH4 not only increased endogenous CH4 production, but also decreased the contents of thiobarbituric acid reactive substances. PEG-induced water deficit symptoms, such as decreased biomass and relative water contents in both root and shoot tissues, were also alleviated. These beneficial responses paralleled the increases in the contents of soluble sugar and the reduced ascorbic acid (AsA), and the ratio of AsA/dehydroascorbate (DHA). Further comparison of transcript profiles of some key enzymes in sugar and AsA metabolism suggested that CH4 might participate in sugar signaling, which in turn increased AsA production and recycling. Together, these results suggested that CH4 might function as a gaseous molecule that enhances osmotic stress tolerance in maize by modulating sugar and AsA metabolism.

No MeSH data available.