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Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

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ABSTRACT

In this study, we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought, using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling, root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves, respectively. Differences between the root and leaf proteome were most marked for photosynthesis, energy metabolism, and protein metabolism. In particular, some DAPs involved in energy and protein metabolism had contrasting accumulation patterns in roots and leaves. Protein-protein interaction (PPI) analysis of roots and leaves revealed complex protein interaction networks that can generate synergistic responses to drought stress and during recovery from drought. Transcript analysis using quantitative real-time polymerase chain reaction (qRT-PCR) validated the differential expression of key proteins involved in the PPI network. Our integrated physiological and proteomic analysis provides evidence for a synergistic network involved in responses to drought and active during recovery from drought, in Brachypodium roots and leaves.

No MeSH data available.


PPI networks under drought stress and recovery in roots and leaves of Bd21.Nodes with bright blue edges represent the important DAPs. KOG0841,14-3-3-like protein A-like; KOG1662,ATP synthase delta chain; KOG1367,Phosphoglycerate kinase; KOG0657,glyceraldehyde-3-phosphate dehydrogenase B; KOG0852,2-Cys peroxiredoxin BAS1; KOG1198,Quinone oxidoreductase-like protein At1g23740; KOG0731,ATP-dependent zinc metalloprotease FTSH 2; KOG0460,elongation factor Tu; KOG0356,Chaperonin CPN60-2; KOG0888,NDPK2; KOG1643,triosephosphate isomerase; KOG0867,glutathione S-transferase 3-like; KOG0441,superoxide dismutase [Cu-Zn] 4A-like; KOG1051,chaperone protein ClpC1; KOG1668,elongation factor 1-beta-like isoform
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f6: PPI networks under drought stress and recovery in roots and leaves of Bd21.Nodes with bright blue edges represent the important DAPs. KOG0841,14-3-3-like protein A-like; KOG1662,ATP synthase delta chain; KOG1367,Phosphoglycerate kinase; KOG0657,glyceraldehyde-3-phosphate dehydrogenase B; KOG0852,2-Cys peroxiredoxin BAS1; KOG1198,Quinone oxidoreductase-like protein At1g23740; KOG0731,ATP-dependent zinc metalloprotease FTSH 2; KOG0460,elongation factor Tu; KOG0356,Chaperonin CPN60-2; KOG0888,NDPK2; KOG1643,triosephosphate isomerase; KOG0867,glutathione S-transferase 3-like; KOG0441,superoxide dismutase [Cu-Zn] 4A-like; KOG1051,chaperone protein ClpC1; KOG1668,elongation factor 1-beta-like isoform

Mentions: In plants, proteins do not function in isolation within cells, but as part of a network37. In this study, a PPI network was generated to highlight interactions and relationships between different proteins. Figure 6 demonstrates that a large proportion of the proteins identified in both roots and leaves were involved in energy metabolism, while cell wall and cell structure associated proteins were important in the PPI network in roots but not in leaves. We found that signal transduction-related proteins interacted with proteins from other functional groups in both the root and leaf PPI networks, but that these were more prevalent in roots than in leaves. We speculate that signaling processes are more active in roots than in leaves responding to drought. In particular, KOG0841 (14-3-3-like protein A) was involved in interactions with proteins from other functional groups in both roots and leaves, suggesting it has an important signaling role in both organs. Similar results were obtained from Bd21 seedlings subjected to H2O2 stress26.


Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery
PPI networks under drought stress and recovery in roots and leaves of Bd21.Nodes with bright blue edges represent the important DAPs. KOG0841,14-3-3-like protein A-like; KOG1662,ATP synthase delta chain; KOG1367,Phosphoglycerate kinase; KOG0657,glyceraldehyde-3-phosphate dehydrogenase B; KOG0852,2-Cys peroxiredoxin BAS1; KOG1198,Quinone oxidoreductase-like protein At1g23740; KOG0731,ATP-dependent zinc metalloprotease FTSH 2; KOG0460,elongation factor Tu; KOG0356,Chaperonin CPN60-2; KOG0888,NDPK2; KOG1643,triosephosphate isomerase; KOG0867,glutathione S-transferase 3-like; KOG0441,superoxide dismutase [Cu-Zn] 4A-like; KOG1051,chaperone protein ClpC1; KOG1668,elongation factor 1-beta-like isoform
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f6: PPI networks under drought stress and recovery in roots and leaves of Bd21.Nodes with bright blue edges represent the important DAPs. KOG0841,14-3-3-like protein A-like; KOG1662,ATP synthase delta chain; KOG1367,Phosphoglycerate kinase; KOG0657,glyceraldehyde-3-phosphate dehydrogenase B; KOG0852,2-Cys peroxiredoxin BAS1; KOG1198,Quinone oxidoreductase-like protein At1g23740; KOG0731,ATP-dependent zinc metalloprotease FTSH 2; KOG0460,elongation factor Tu; KOG0356,Chaperonin CPN60-2; KOG0888,NDPK2; KOG1643,triosephosphate isomerase; KOG0867,glutathione S-transferase 3-like; KOG0441,superoxide dismutase [Cu-Zn] 4A-like; KOG1051,chaperone protein ClpC1; KOG1668,elongation factor 1-beta-like isoform
Mentions: In plants, proteins do not function in isolation within cells, but as part of a network37. In this study, a PPI network was generated to highlight interactions and relationships between different proteins. Figure 6 demonstrates that a large proportion of the proteins identified in both roots and leaves were involved in energy metabolism, while cell wall and cell structure associated proteins were important in the PPI network in roots but not in leaves. We found that signal transduction-related proteins interacted with proteins from other functional groups in both the root and leaf PPI networks, but that these were more prevalent in roots than in leaves. We speculate that signaling processes are more active in roots than in leaves responding to drought. In particular, KOG0841 (14-3-3-like protein A) was involved in interactions with proteins from other functional groups in both roots and leaves, suggesting it has an important signaling role in both organs. Similar results were obtained from Bd21 seedlings subjected to H2O2 stress26.

View Article: PubMed Central - PubMed

ABSTRACT

In this study, we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought, using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling, root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves, respectively. Differences between the root and leaf proteome were most marked for photosynthesis, energy metabolism, and protein metabolism. In particular, some DAPs involved in energy and protein metabolism had contrasting accumulation patterns in roots and leaves. Protein-protein interaction (PPI) analysis of roots and leaves revealed complex protein interaction networks that can generate synergistic responses to drought stress and during recovery from drought. Transcript analysis using quantitative real-time polymerase chain reaction (qRT-PCR) validated the differential expression of key proteins involved in the PPI network. Our integrated physiological and proteomic analysis provides evidence for a synergistic network involved in responses to drought and active during recovery from drought, in Brachypodium roots and leaves.

No MeSH data available.